CRISPR 101: Multiplex Expression of gRNAs
Type
Blog Post
Published
Sept. 10, 2020, 11:45 a.m.
...creating flanking overhangs. These overhangs can be customized to link together multiple fragments, allowing...1x4; for 6 gRNAs, you’d use pX330A-1x6. This customization means you don’t ever need to clone in filler...plasmids allow expression of 2-5 gRNAs in zebrafish. Custom destination vectors are used depending upon the...