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...genomic modifications, is very robust in C. elegans, and the methods for HDR-mediated modification continue...permission from the Genetics Society of America. Applications of this system Dickinson et al. have developed...Used with permission from the Genetics Society of America. To use this SEC-based system, first design...use and highly efficient for C. elegans genome modification. Figure 2: Schematic from Dickinson et...system could be used to make many other genome modifications, including targeted mutations. SEC-based systems... in cell culture. In addition to CRISPR/Cas9 applications, SEC selection could replace other, more restrictive... of print] PubMed. Find plasmids from this publication at Addgene. Other strategies for CRISPR/Cas9...

...mammalian cells often relied on chemical (CaCl2, cationic liposome) or electrical (electric current forming ...research since their first use in the early 90’s to genetically modify primary cells. Amongst the different vectors...particles and the virus particles produced could not replicate. However, Mother Nature found a way to re-create...packaging plasmid forming a vector that could create replication competent virus. Even though this system was ...its safety was a disadvantage particularly for clinical development. In addition, MoMLV is inefficient...still a potential risk for the generation of replication competent lentiviruses (RCL) especially if you...factors in HIV positive cells could make the virus replication competent. The 3rd generation of lentiviral...

...post was updated on Jul 27, 2020. CRISPR, and specifically Cas9 from S. pyogenes (SpCas9), is truly an ...use, functional in most species, and has many applications. That said, SpCas9 is not the only game in town... makes this molecule unique, and the various applications of Cas13a. The origins of Cas13a: An RNA cleaving...specified by the crRNA sequence, it adopts an enzymatically “active” state rather than reverting to an inactive...discussed below. Subscribe to CRISPR Blog Posts Applications of Cas13a   Figure 1: Using Cas13a as...) is amplified using Recombinase Polymerase Amplification (RPA) or Reverse-transcription RPA (RT-RPA),...fluorophore. The fluorescent signal can be used as an indicator to determine whether the target sequence is present...

...skills The ability to think critically and carry out in-depth research is applicable to a wide range of industries...many opportunities available to those who are scientifically minded! Listen to Biotech Founder Michael Koeris...Scientific Advisor Lab Manager Patent Lawyer Technical Writer Meteorologist Journal Editor Textile ...organised, reliable, and can work independently. Practical outdoor skills are needed in a variety of roles...Breeder Scientific Illustrator Plant Breeder Zoological Worker Conservationist Documentary Director ...range of roles, including: Science Reporter Technical Writer Statistician Bioinformatician Science...Marketer Software Developer Learn Science Communication Skill from a Student Group Like Science in the...

...overall. Communication is the issue Much of the problem can be attributed to communication, and in particular...particular communication about experimental methods and approaches. It’s very easy to think that the methods...that they were doing something differently. Communication comes in many forms—from transparency about ...them troubleshoot and understand. All of this communication and sharing fits together to make for a transparent...for pushing for more transparency in science communication falls on the shoulders of journals and publishers...can feel like yet another hurdle in front of publication when all they really want to do is get the paper...we take our responsibility to push for clear communication of science very seriously. That’s why at Cell...

...tropism, replication kinetics, and cell damage profiling of SARS-CoV-2 and SARS-CoV with implications for ... from their original sources. Figure 1: Biological repositories that distribute mouse strains, plasmids... plasmids, cell lines, and much more have been critical for pandemic research. Image from Reproducibility...this virus. One such example is cell lines. The American Type Culture Collection (ATCC) is a private, nonprofit...Cell Bank, part of the National Institutes of Biomedical Innovation, Health, and Nutrition in Japan. These... Another example of reagents that have broad applications but have proven useful to the study of SARS-...vectors VSV-G and psPAX2, which were used in a publication by Xiuyuan Ou, et al. that characterized the ...

...cells and statistically analyze cell movements. By using cells labelled by fluorescent chemicals or by other... steady state or under physio-pathological challenge. However technical specificities have to be taken... required for a growing organism to make the complicated transition from a clump of cells to a fully differentiated...mobility than the static microscopy images of chemically coloured lymphoid organs did in the past. How...fluorescent protein or dye. It separates the cells mechanically in a vibrating nozzle, imparting a positive ... millimeter in depth. Two low-energy photons (typically from the same laser) cooperate to cause a higher-energy...reporter constructs in the immune lineage. By specifically labeling T cells, B cells, and antigen presenting...

...target antigen as canonical antibodies. They also have the advantage of being significantly smaller, meaning...binding to cell surface receptors. These applications are typically used for investigating signaling pathways...tool that can be used for a variety of research applications. Within the immune system, antibodies are produced...antibodies to bind proteins is useful for research applications as well because they allow scientists to target...Epitopes are between 5-8 amino acids long, with a typical length of 5 or 6 amino acids (Cruse et al., 2004...recognize epitopes that full antibodies would not physically be able to reach. While a full antibody is ~150...150 kDa, a nanobody is 12-15 kDa.   Research applications for antibodies You can use antibodies for experiments...

...colleges, private universities, and medical schools. I will emphatically deny that I have a magic formula..." manual on applying for faculty positions in biomedical science. My motivation was to share my experiences... do need multiple first/corresponding author publications in quality journals. It helps to come from the...a job. The currency of scientific success is publications and grants. 2. Write succinct and easy to understand...told all of the nuances of the research in as technical of language as possible. Seriously. My suggested...humbling. My conviction that I had a flair for communication was rapidly dispelled. She would ask me, "Is... trying to say X." She would then look at me quizzically and ask why I didn't just say that? Bottom line...

...recombinase polymerase amplification (RPA) using a commercially available amplification kit. In this case,... RT-qPCR tests to diagnose COVID-19 and some serological tests to determine past exposure. However, the... RNA is extracted and subject to isothermal amplification by primers that target the E and the N2 gene...reverse transcriptase to produce cDNA. Isothermal amplification is done at one constant temperature, eliminating...rapid detection.  Highlights: Cas13a is not catalytically active in the presence of two or more mismatches...that exhibit fluorescence upon cleavage. Upon amplification by the RPA primers, the Cas12a-crRNA binds to... SARS-CoV-2 detection, AIOD-CRISPR without preamplification was able to detect as low as 1.2 copies DNA...