We narrowed to 533 results for: Erf

...G)? You may wonder if a CRISPR-base editor can perform this change. However, there are no naturally known...

...enough information there for you to be independent performing the technique you trained to do? Walk through...

...dilute the unintegrated plasmids (Fig. 2b). Next, perform a short (12-hour) induction with doxycycline prior...

...discovery, plasmid addiction systems have become a powerful tool to address a diverse set of biological inquiries...

... in your expression host of choice, you should perform a functional assay to make sure your protein isn...

...identified (Hart et al, Bassik et al). The library performed well in both screens, with the growth screen having...

...you realize. Being at a university provides the perfect platform for practicing initiative and for training...

...strand breaks it introduces makes it difficult to perform two or more integrations on the genome. Most recently...

...from Leptotrichia wadei (LwaCas13a), using a superfolder GFP fusion to stabilize the protein in mammalian...

...veggies) Though the aptabeacons can be used as powerful biosensors, the bound protein fluorophores and...

...difficult to obtain. These can then be used to perform cell therapy and to model diseases in vitro. Find...

... vector.  Once the BP and/or LR reactions are performed, the next step is to transform competent E. coli...

... for diagnosing and resolving organizational performance and often have to be able to quickly synthesize...

...publically about your work (you can find my tips for a perfect science talk here). Finally, my main content organizer...

...generate your pCAG-EGXXFP plasmid? They are the perfect primer sets to use to genotype your pups. A simple...

...making it easier for comparable studies to be performed on a cell line even decades after it is initially...

...Lentiviruses are a powerful laboratory tool often employed to establish cell lines that stably express...

...in the laboratory mouse, most optogenetics is performed in this species. However, transgenic rat technology...

... expression from high-copy number plasmids and powerful promoters will greatly exceed that of any native...

...a good place to start when trying to pick your perfect promoter! Eukaryotic Promoters   Promoter   ...