We narrowed to 588 results for: BASE

...Fluorescence fLuctuation INcrease by Contact (FLINC)-based biosensors measure fluctuations of TagRFP-T fluorescence...

...used to restrict expression to a single cell type based on the overlap in cell types both promoters expressed...Institute of Molecular and Clinical Ophthalmology Basel recently took a different approach to enhance specificity...targeting the same cell class in humans (R=0.67).  A database of the microscopy images of all AAVs that reproducibly...

...side of the optogenetics coin is materials science-based. Light is delivered to your opsin through a small...

...a final list of questions for the actual event, based on these interviews. The idea is to ask questions...

...you can extract practically every single reliable base pair from your results. This option is not as fast...sequence databases from the dropdown menu. Typically, we use the default nucleotide database “Nucleotide...plasmids with us or other entries in NCBI’s numerous databases. As our repository has grown over the years (we... explanation of the different BLAST programs, databases, and elements of the BLAST search pages. At Addgene...known reference sequence or to a BLAST sequence database. If you know the expected nucleotide sequence,...identity between your sequence and the reference/database sequence is ≥ 95%. [Our QC process would be trouble-free...blastx checks all six possible frames against the database. Another benefit is that a frameshift mutation...

...journal clubs have become popular, including those based on Twitter. At my former academic department, we...

...acceptor for fluorescence lifetime imaging microscopy-based Förster resonance energy transfer (FLIM-FRET) in...

...PMID: 27381852. Choi, Kyoung-Hee, et al. "A Tn7-based broad-range bacterial cloning and expression system...

...vector delivery is often used, the CRISPR approach based on HDR has the advantage of being a potentially ...

...Antibodies Plasmids 101: Peptide Tagging Plasmid-based Recombinant Antibody Resources on Addgene.org  ...

...manager: Unlike academia, promotions in industry are based on people’s ability to manage others and work as...

... target cell surface receptors (Callaway 2008). Based on this, cell type specificity of the starter cell...

...populations.   Figure 2: Compensation example based on experimental sample. Flow plots showing a sample...

...in-frame to the protein of interest for plasmid-based expression systems. Degron tags are one of many ...

...neutralized by pre-existing antibodies against AAV9-based vectors (and vice versa), enabling repeat dosing...

...Voltage Sensitive Domain (VSD) GEVIs Simply put, VSD-based sensors consist of a VSD fused to a single fluorescent...

...clozapine-N-oxide, clozapine, and compound 21 in DREADD-based chemogenetics in mice. Scientific Reports 9: https...

...to help you learn more about fly biology and fly-based experimental systems. Some resources will direct...book for newbies on how to work with flies. Flybase.org is absolutely essential. You can look up information...many fly stocks that are available to everyone. Flybase lists what stocks are available at Bloomington,...

...find themselves plagued by troubleshooting a virus-based experiment at one point or another. After amplifying...

...the Plasmid Map: We auto-generate a plasmid map based off of the plasmid sequence and feature information... create their new plasmid.  The Addgene Vector Database has more information about common vector backbones...