We narrowed to 577 results for: BASE
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TypeBlog PostPublishedDec. 10, 2020, 1 p.m....vector delivery is often used, the CRISPR approach based on HDR has the advantage of being a potentially ...
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Hot Plasmids February 2024
TypeBlog PostPublishedFeb. 20, 2024, 2:45 p.m....neutralized by pre-existing antibodies against AAV9-based vectors (and vice versa), enabling repeat dosing... -
Viral Vectors 101: Voltage Indicators
TypeBlog PostPublishedJan. 30, 2024, 2:15 p.m....Voltage Sensitive Domain (VSD) GEVIs Simply put, VSD-based sensors consist of a VSD fused to a single fluorescent... -
Viral Vectors 101: Chemogenetics
TypeBlog PostPublishedJuly 11, 2023, 1:15 p.m....clozapine-N-oxide, clozapine, and compound 21 in DREADD-based chemogenetics in mice. Scientific Reports 9: https... -
Antibodies 101: Flow Compensation
TypeBlog PostPublishedMay 16, 2024, 1:15 p.m....populations. Figure 2: Compensation example based on experimental sample. Flow plots showing a sample... -
Plasmids 101: Degron Tags
TypeBlog PostPublishedMarch 14, 2024, 1:15 p.m....in-frame to the protein of interest for plasmid-based expression systems. Degron tags are one of many ... -
Of Myc and Men
TypeBlog PostPublishedJan. 19, 2023, 2:15 p.m....Antibodies Plasmids 101: Peptide Tagging Plasmid-based Recombinant Antibody Resources on Addgene.org ... -
A Guide to Starting Your Own Journal Club
TypeBlog PostPublishedFeb. 6, 2020, 3 p.m....journal clubs have become popular, including those based on Twitter. At my former academic department, we... -
Chromoproteins: Colorful Proteins For Molecular Biology Experiments
TypeBlog PostPublishedFeb. 4, 2020, 2:15 p.m....acceptor for fluorescence lifetime imaging microscopy-based Förster resonance energy transfer (FLIM-FRET) in... -
Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More
TypeBlog PostPublishedJune 12, 2014, 3:30 p.m....Hopefully we will have new tools for plant science based around those technologies in the near future. Science... -
New Tools Enable CRISPRa for Neuroscience Applications
TypeBlog PostPublishedJuly 2, 2019, 12:51 p.m....application to neuroscience research. What is CRISPR-based activation? To summarize, CRISPRa uses a nuclease-dead... -
Lab to Office Culture Shock
TypeBlog PostPublishedFeb. 5, 2019, 1:04 p.m....desk jobs “alternative careers.” Realistically, based on the numbers of PhDs and available positions alone... -
Plasmids 101: Codon usage bias
TypeBlog PostPublishedSept. 27, 2018, 1:09 p.m....amino acids. This is possible because there are 4 bases (A, T, C, and G) and 3 positions in each codon. ... at all in E. coli (you can find a variety of databases showing various organisms’ codon preferences online... -
Transferable Skills Guide: Identifying Your Transferable Skills
TypeBlog PostPublishedJan. 11, 2018, 3:29 p.m....together a really great solution that covered all the bases. You may have also used your writing skills to convey... -
AAVs CREATed for Gene Delivery to the CNS and PNS
TypeBlog PostPublishedSept. 28, 2017, 2:01 p.m....CREATE method? CREATE stands for Cre Recombinase-based AAV Targeted Evolution. In this technique, a pool... -
Healthcare Consulting: A Door to the Business of Life Sciences
TypeBlog PostPublishedOct. 13, 2016, 2:30 p.m....resources towards the development of a new therapy based on its market need and anticipated commercial success... -
Better Dyeing Through Chemistry & Small Molecule Fluorophores
TypeBlog PostPublishedSept. 8, 2016, 2:30 p.m.... 1a). Other strategies that have been developed based off of this concept include: Self-labeling tags... -
When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells
TypeBlog PostPublishedApril 19, 2016, 2:30 p.m....Ohashi, T., et al., An experimental study of GFP-based FRET, with application to intrinsically unstructured... -
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy
TypeBlog PostPublishedAug. 27, 2020, 1:15 p.m....over the other BiFC is a convenient, fluorescence-based method for directly visualizing PPIs in cells. The... -
Negative Can Be Positive: Open AAV Data with Addgene
TypeBlog PostPublishedSept. 6, 2022, 1:15 p.m....appropriately credited, broadening the knowledge base of AAV vector performance in various brain regions...