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We narrowed to 847 results for: CAL;

Showing: 481 - 500 of 847 results

  1. FastCloning: A Newer, Simpler Cloning Technique

    Type
    Blog Post
    Published
    Nov. 1, 2016, 2:30 p.m.
    ...techniques in the biological sciences and constitute foundational elements of biomedical research. Traditional...Digestion products are then transformed directly into chemically competent E. coli and transformants selected ...procedure to work properly. Directly transform chemically competent cells with the digested mixture. There...

  3. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    Published
    June 30, 2015, 2 p.m.
    ...gene therapy using Sleeping Beauty to genetically modify clinical-grade T cells to target CD19. Immunol...DNA while eliminating some of the drawbacks. Specifically, viruses are able to infect and replicate in...Anderson Cancer Center presented results from a clinical trial using the Sleeping Beauty transposase as...

  4. Choosing a Good Mentor for Scientists

    Type
    Blog Post
    Published
    Jan. 16, 2014, 2:56 p.m.
    ...problems, develop general technical proficiency, communicate your work, maintain ethical standards and interact...? Listen to our podcast interview with Harvard Medical School researcher Connie Cepko to learn about her... you admire her management style? Does he have ethical behavior? Does the lab encourage diversity? Knowing...

  5. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    Published
    Nov. 25, 2013, 10:12 p.m.
    ... tactics of prokaryotes to deliver the technological biological blockbuster known as the CRISPR (clustered...destroy the phage upon re-exposure (2, 3). More specifically, specialized Cas proteins snip the foreign DNA...programmable platform well-suited for various innovative biological and translative technologies. Addgene has empowered...

  6. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    Published
    June 9, 2022, 1:15 p.m.
    ... defined learning program. Learners cannot (theoretically, at least) fully and freely engage or disengage...Informal education is education that is provided, typically ad hoc, for learners who can choose to engage ...would use “PCR test” to mean “a test that uses a biological sample to look for presence of a virus.” A scientist... scientist underrepresented minorities in your local high school who are also students in AP biology ...

  7. RUBY-Red Siliques

    Type
    Blog Post
    Published
    March 1, 2022, 2:15 p.m.
    ...editing of transformants then typically occurs in T0 plants (from callus) or T1 plants (Arabidopsis floral...involves killing the untransformed seedlings or callus (undifferentiated plant cell culture), which is...cacti and beets. Betanin belongs to a different chemical family than the red/purple plant pigments found...

  8. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type
    Blog Post
    Published
    April 25, 2019, 3:01 p.m.
    ...FRET reporters. mTFP1 is also insensitive to physiologically relevant changes in pH. Find mTFP1 plasmids...Fluorescent proteins that oligomerize often affect the localization and/or function of the protein that they are...fluorescent protein and found that the fusion protein localized to their expected locations. An analysis of mWasabi...characterization and applications in fluorescence imaging." Biochemical Journal 400.3 (2006): 531-540. PubMed PMID: 16859491...

  9. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    Published
    July 21, 2022, 1:15 p.m.
    ... years of exclusively writing lab reports and empirical essays had also stirred some of my more creative...exhibitions, animation, graphic design, and more. I recall a particularly busy point of the first semester... notion of “heading” in soccer as a possible neurological health risk. Having played the sport my entire...among collision sport athletes, as well as the ethical and health policy implications arising from sport-related...

  10. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    Published
    Oct. 27, 2016, 2:30 p.m.
    ...bonds. This post focuses on "sticky end" TOPO (also called TOPO-TA) cloning; however, the TOPO cloning technique...to facilitate replication. The TOPO technique specifically uses Vaccinia virus-isolated topoisomerase I... has an error rate of about 1 in 3,500 bases. Typically polymerases with proofreading functionality are...are planning on transforming your reaction into chemically or electro-competent E. coli - excess salt causes...

  11. Donations from Addgene to Yield Answers for Rare Disease Researchers

    Type
    Blog Post
    Published
    Sept. 15, 2015, 2:30 p.m.
    ... PA will put their award to work on a condition called xeroderma pigmentosum (XP). This recessive genetic...gene encodes a sodium-potassium pump expressed specifically in neurons of the brain. McSweeney says they...Wattanasirichaigoon carried different mutations in a gene called SOX10. To understand the functional role of those...models carrying different SOX10 mutations. More specifically, they’ve proposed to use plasmids with modified...

  12. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    Published
    April 7, 2015, 4:21 p.m.
    ... The conventional approach has typically been to either systematically delete/overexpress endogenous genes... interference (CRISPRi) model consists of a catalytically dead Cas9 protein (dCas9) that lacks endonuclease...Western Ontario studying plant-based recombinant pharmaceuticals. Follow him on Twitter @AdamChinFatt     References...

  13. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

    Type
    Blog Post
    Published
    June 1, 2021, 1:15 p.m.
    ...test if a type of antibodies from an Andean mammal called alpaca could neutralize the SARS-CoV-2 virus. But...Then, one of them heard about our online platform, called Crowdfight, and filed a request for help. In just... stage of the project we are focusing on what we call “asymmetric collaborations.” These are transient...-and-error. A recommendation for the correct statistical test can save a retraction. Today, collaborations...

  14. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    Published
    June 18, 2019, 1:15 p.m.
    ...Control AAVs for serotype testing jGCaMP7c for calcium detection PSAM4 GlyR for chemogenetic inhibition...high contrast between its resting state and its calcium-bound state, which was achieved by lowering the...  AAV1  pAAV.hSynapsin.SF-iGluSnFR.S72A New calcium sensors Plasmid Serotype Name 104496  AAV1...fluorescent proteins can affect DREADD trafficking and localization. The Sternson lab recently deposited PSAM AAV...

  15. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type
    Blog Post
    Published
    May 21, 2019, 12:10 p.m.
    ...cell death" plays a pivotal role in an array of biological processes including development, the immune system...study apoptosis in vivo, scientists have used genetically encoded fluorescent executioner caspase reporters... reporter for imaging apoptosis in vivo." Cell chemical biology 23.7 (2016): 875-882. PubMed PMID: 27447051...apoptosis in animals." Journal of the American Chemical Society (2019). PubMed PMID: 30821975. Additional...

  16. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    Published
    April 18, 2019, 12:53 p.m.
    ...The catalytically dead Cas9 protein (dCas9) is well known for its ability to bind DNA targets without...Pederson’s lab at the University of Massachusetts Medical School developed a dCas9-based multicolor labeling...variants, SpCas9, NmCas9, and St1Cas9. These fusions localized to specific loci based on the guide RNA sequence...RFP) to the genomic loci of interest. Pairs of identical hairpins within one gRNA will result in a blue...

  17. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    Published
    March 14, 2019, 1:54 p.m.
    ...from Massachusetts General Hospital and Harvard Medical School took a closer look at the AAV vectors that...Purifying exo-AAVs is actually a bit simpler than the classical purification protocol involving iodixanol gradients...exo-AAVs seem to target cell types with a similar specifically as conventionally produced AAV particles. But... Tetraspanin CD9." Molecular Therapy-Methods & Clinical Development 9 (2018): 278-287. PubMed PMID: 29707602...

  18. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    Published
    Sept. 8, 2015, 2:30 p.m.
    ...to directly activate and inactivate biological processes. Chemical effectors are suboptimal for a number...neuroscience. In fact, precise light-based control of biological processes is highly useful in other fields, including...system to develop a bacterial camera that prints a chemical image, as well as a genetic method for the computational...

  19. Management for Scientists: Delegating is Key

    Type
    Blog Post
    Published
    Feb. 20, 2015, 8:30 p.m.
    ... outcomes. The quality of the work is often dramatically improved when tasks are distributed appropriately...the project completed by an individual who is a local expert in that task (or has the bandwidth to become.... Listen to our podcast interview with Harvard Medical School researcher Connie Cepko to learn about her...recommend putting a regular check-in meeting on the calendar even if it is just 15 minutes a week. Clearly...

  20. Advice for Scientists Starting a Lab

    Type
    Blog Post
    Published
    April 29, 2014, 1:43 p.m.
    ... Connie Cepko (Harvard Medical School) , and Dr. George Church (Harvard Medical School) for taking the...your department, and online. The Howard Hughes Medical Institute (HHMI) offers some great resources for...Scientists, including their "Making the Right Moves: A Practical Guide to Scientifıc Management for Postdocs and...
Showing: 481 - 500 of 847 results