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We narrowed to 538 results for: CAR

Showing: 481 - 500 of 538 results
  1. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    Published
    Oct. 18, 2018, 12:37 p.m.
    ...below. The main requirement for IVA cloning is carefully designed primers. The majority of mistakes are...
  2. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ...Its main limitation is the small size of its DNA cargo - 4.8 kb at most - which means that a gene larger...
  3. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    ...2007-02-062117. PMID: 17510320. Tollefson, A. E., Scaria, A., Hermiston, T. W., Ryerse, J. S., Wold, L. ...
  4. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ...distortions to a minimum with protocol optimization and careful execution, but they cannot be entirely eliminated...
  5. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    Published
    Jan. 16, 2020, 2:18 p.m.
    ... manual droplet generator, you will have to be careful about transferring your droplets from the droplet...
  6. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ...how will you use these systems in your research? Cary Valley contributed to updating this article. References...
  7. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    Published
    Dec. 22, 2022, 2:15 p.m.
    ...hours, so read up on the timing of this approach carefully (Lin et al., eLife)! Use a Cas that facilitates...
  8. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    Published
    Feb. 27, 2018, 2:04 p.m.
    ...with a reporter enzyme. For multicolor staining, careful consideration must be used when selecting which...
  9. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ...line. When expressing a viral construct transiently care needs to be taken to ensure that the proper cell...
  10. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ...markers tagged with mTurquoise2, mNeonGreen, and mScarlet-I for labeling a list of specific structures within...
  11. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    Published
    Feb. 17, 2022, 2 p.m.
    ...followed bylanding site recognition and integration of cargo via Cas9-directed integrases.   Use PASTE can...
  12. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    Published
    Jan. 23, 2020, 2:45 p.m.
    ...Figure 1: Addgene’s Agile Approach Sharing is caring There are many moving parts to our software, which...
  13. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    Published
    July 15, 2025, 1:15 p.m.
    ...significant contributions from Alyssa Cecchetelli, Cary Valley, and Holly McQueary References and Resources...
  14. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ...you realize events are plotted on an invisible Cartesian plane (right.) Each event is plotted by the amount...
Showing: 481 - 500 of 538 results