We narrowed to 534 results for: CAR
-
TypeBlog PostPublishedJune 20, 2023, 1:15 p.m....distortions to a minimum with protocol optimization and careful execution, but they cannot be entirely eliminated...
-
CRISPR 101: Making a Knock-In Cell Line
TypeBlog PostPublishedDec. 22, 2022, 2:15 p.m....hours, so read up on the timing of this approach carefully (Lin et al., eLife)! Use a Cas that facilitates... -
CRISPR 101: RNA Editing with Cas13
TypeBlog PostPublishedJuly 31, 2020, 12:30 p.m....how will you use these systems in your research? Cary Valley contributed to updating this article. References... -
Droplet Digital PCR for AAV Quantitation
TypeBlog PostPublishedJan. 16, 2020, 2:18 p.m.... manual droplet generator, you will have to be careful about transferring your droplets from the droplet... -
Plasmids 101: Secondary Nanobody Toolbox
TypeBlog PostPublishedFeb. 27, 2018, 2:04 p.m....with a reporter enzyme. For multicolor staining, careful consideration must be used when selecting which... -
Uncovering Ancestral Bias in CRISPR Research
TypeBlog PostPublishedNov. 29, 2022, 2:15 p.m....science and society." Science 376, (2022): 250-252. Carlson J, Henn BM, Al-Hindi DR, Ramachandran S. Counter... -
Deep Dive: Fixing and Permeabilizing for Immunofluorescence
TypeBlog PostPublishedAug. 30, 2022, 1:15 p.m.... the outcome of your IF experiment and deserve careful thought and attention. * There are other methods... -
Tips for Screening with Yeast Two Hybrid Systems
TypeBlog PostPublishedOct. 22, 2015, 2:30 p.m....experiments must be set up, conducted and analysed with care to get reliable information. Although two-hybrid... -
Your Lentiviral Plasmid FAQs Answered
TypeBlog PostPublishedApril 23, 2014, 1:08 p.m....line. When expressing a viral construct transiently care needs to be taken to ensure that the proper cell... -
"Hall of Fame" AAV Enhancers from the Allen Institute for Brain Science
TypeBlog PostPublishedSept. 9, 2025, 1:15 p.m....continue to develop improved, reliable tools to help carry out that research. Organizations like the Allen ... -
QC Sequencing Technologies at Addgene
TypeBlog PostPublishedJuly 15, 2025, 1:15 p.m....significant contributions from Alyssa Cecchetelli, Cary Valley, and Holly McQueary References and Resources... -
Antibodies 101: Reading a Flow Plot
TypeBlog PostPublishedFeb. 6, 2024, 3:13 p.m....you realize events are plotted on an invisible Cartesian plane (right.) Each event is plotted by the amount... -
Fluorescent Proteins 101: Aptamer Fluorophores
TypeBlog PostPublishedApril 11, 2017, 2:30 p.m....up with new colors (Fig. 3), such as Radish and Carrot, and further means to increase aptamer stability... -
Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names
TypeBlog PostPublishedApril 2, 2019, 1:44 p.m....and muscle contractions (think skeletal muscle, cardiac muscle, and your airways and blood vessels). Spaghetti... -
New CRISPR Tools: Cas7-11 and PASTE
TypeBlog PostPublishedFeb. 17, 2022, 2 p.m....followed bylanding site recognition and integration of cargo via Cas9-directed integrases. Use PASTE can... -
Rett Syndrome: A History of Research and Therapeutic Outlooks
TypeBlog PostPublishedNov. 19, 2020, 2:15 p.m....may exhibit slow head growth, develop seizures, cardio-respiratory dysfunction, distinctive hand movements... -
Overcoming the AAV Size Limitation for CRISPR Delivery
TypeBlog PostPublishedSept. 16, 2020, 1 p.m....it doesn’t eliminate the need to deliver larger cargo such as base editors and prime editors. While using... -
Hot Plasmids - August 2020
TypeBlog PostPublishedAug. 18, 2020, 1:15 p.m....markers tagged with mTurquoise2, mNeonGreen, and mScarlet-I for labeling a list of specific structures within... -
An Inside Look into Addgene's Dev Team
TypeBlog PostPublishedJan. 23, 2020, 2:45 p.m....Figure 1: Addgene’s Agile Approach Sharing is caring There are many moving parts to our software, which... -
How to Write a Scientific Review Article
TypeBlog PostPublishedFeb. 16, 2017, 3:30 p.m....the themes in the field and to learn what people cared about. I also used reviews to get a list of research...