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We narrowed to 539 results for: CAR

Showing: 481 - 500 of 539 results
  1. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    Published
    Dec. 22, 2022, 2:15 p.m.
    ...hours, so read up on the timing of this approach carefully (Lin et al., eLife)! Use a Cas that facilitates...
  2. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ...how will you use these systems in your research? Cary Valley contributed to updating this article. References...
  3. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    ...2007-02-062117. PMID: 17510320. Tollefson, A. E., Scaria, A., Hermiston, T. W., Ryerse, J. S., Wold, L. ...
  4. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ...distortions to a minimum with protocol optimization and careful execution, but they cannot be entirely eliminated...
  5. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    Published
    Jan. 16, 2020, 2:18 p.m.
    ... manual droplet generator, you will have to be careful about transferring your droplets from the droplet...
  6. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    Published
    Oct. 18, 2018, 12:37 p.m.
    ...below. The main requirement for IVA cloning is carefully designed primers. The majority of mistakes are...
  7. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ...Its main limitation is the small size of its DNA cargo - 4.8 kb at most - which means that a gene larger...
  8. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    Published
    Feb. 27, 2018, 2:04 p.m.
    ...with a reporter enzyme. For multicolor staining, careful consideration must be used when selecting which...
  9. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ...line. When expressing a viral construct transiently care needs to be taken to ensure that the proper cell...
  10. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    Published
    Feb. 17, 2022, 2 p.m.
    ...followed bylanding site recognition and integration of cargo via Cas9-directed integrases.   Use PASTE can...
  11. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    Published
    July 15, 2025, 1:15 p.m.
    ...significant contributions from Alyssa Cecchetelli, Cary Valley, and Holly McQueary References and Resources...
  12. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ...markers tagged with mTurquoise2, mNeonGreen, and mScarlet-I for labeling a list of specific structures within...
  13. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ...you realize events are plotted on an invisible Cartesian plane (right.) Each event is plotted by the amount...
Showing: 481 - 500 of 539 results