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We narrowed to 623 results for: Dos;

Showing: 481 - 500 of 623 results
  1. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    Published
    June 4, 2024, 1:15 p.m.
    ...domains—including the CTD and the entire REC lobe—do not structurally resemble any other known proteins...check for sequence matches; a perfect guide RNA will do you no good if there is no adjacent PAM to anchor...
  2. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    Published
    Jan. 6, 2017, 3:56 p.m.
    ...lentiviral inventory is based on CRISPR tools, though we do provide a few non-CRISPR related viral preps. Cas9...
  3. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    Published
    Jan. 16, 2018, 3:20 p.m.
    ...blogger, Eugenia Rojas. A question worthy of a PhD: How do you visualize protein turnover within a neuron? For...
  4. Summer SciComm: Preprints

    Type
    Blog Post
    Published
    Aug. 16, 2022, 1:15 p.m.
    ...broader community, and preprints are a great way to do that. We are happy to receive deposits of plasmids...
  5. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    Published
    July 25, 2023, 1 p.m.
    ...and are slightly different in sequence. Both LTRs do share common repeat regions in addition to their ...virion assembly and particle release from late endosomes when AAV is trafficked. Rep and cap mediate AAV...
  6. All in a Twist: dsRNA

    Type
    Blog Post
    Published
    Feb. 2, 2023, 2:15 p.m.
    ...produced dsRNA rarely reach this threshold and thus do not typically activate an inflammatory response. ...
  7. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    Published
    July 14, 2021, 1:15 p.m.
    ...strength of staining and background noise. If you do use different lots of your polyclonal antibody, you...
  8. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    Published
    March 3, 2020, 2:15 p.m.
    ...sensors in terms of response and brightness. They also do not spectrally separate from green sensors and blue-light-activated.... The dosing required to achieve these efficiencies was also between 1013 - 1014 vg/kg, a dosage comparable...
  9. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    Published
    Jan. 14, 2016, 3:30 p.m.
    ...sequences to visually check for homology and mismatches. Do you have your own best sequencing practices or maybe...
Showing: 481 - 500 of 623 results