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Showing: 481 - 500 of 620 results

  4. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    Published
    Jan. 6, 2017, 3:56 p.m.
    ...lentiviral inventory is based on CRISPR tools, though we do provide a few non-CRISPR related viral preps. Cas9...

  5. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    Published
    March 3, 2020, 2:15 p.m.
    ...sensors in terms of response and brightness. They also do not spectrally separate from green sensors and blue-light-activated.... The dosing required to achieve these efficiencies was also between 1013 - 1014 vg/kg, a dosage comparable...

  9. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    Published
    Jan. 16, 2018, 3:20 p.m.
    ...blogger, Eugenia Rojas. A question worthy of a PhD: How do you visualize protein turnover within a neuron? For...

  11. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    Published
    July 25, 2023, 1 p.m.
    ...and are slightly different in sequence. Both LTRs do share common repeat regions in addition to their ...virion assembly and particle release from late endosomes when AAV is trafficked. Rep and cap mediate AAV...

  12. All in a Twist: dsRNA

    Type
    Blog Post
    Published
    Feb. 2, 2023, 2:15 p.m.
    ...produced dsRNA rarely reach this threshold and thus do not typically activate an inflammatory response. ...

  17. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    Published
    Jan. 14, 2016, 3:30 p.m.
    ...sequences to visually check for homology and mismatches. Do you have your own best sequencing practices or maybe...

  19. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    Published
    April 16, 2015, 3:45 p.m.
    ... ends better substrates for ligation. Although we do not describe these steps here, the processing of ...

  20. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...Have you used the Cre-lox system in your research? Do you have any tips or tricks, or clever cre-dependent...
Showing: 481 - 500 of 620 results