We narrowed to 623 results for: Dos;
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TypeBlog PostPublishedJune 4, 2024, 1:15 p.m....domains—including the CTD and the entire REC lobe—do not structurally resemble any other known proteins...check for sequence matches; a perfect guide RNA will do you no good if there is no adjacent PAM to anchor...
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How Dry Ice Affects Viral Vector Sample pH & How to Avoid It
TypeBlog PostPublishedJune 20, 2017, 2:30 p.m.... mitigating the pH effects of dry ice storage. To do this, we stored samples on dry ice for 72 hours and... -
Choosing the B(right)est Fluorescent Protein: Aggregation Tendency
TypeBlog PostPublishedJune 15, 2017, 2:30 p.m....with the microscope that you will be using. If you do a head-to-head comparison right, it will be valuable... -
Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection
TypeBlog PostPublishedMay 11, 2017, 2:30 p.m....Central PMCID: PMC5120861. While there is much work to do, these early pilot studies have demonstrated technical... -
Top Requested Lentivirus and AAV of 2016
TypeBlog PostPublishedJan. 6, 2017, 3:56 p.m....lentiviral inventory is based on CRISPR tools, though we do provide a few non-CRISPR related viral preps. Cas9... -
Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number
TypeBlog PostPublishedSept. 4, 2018, 12:53 p.m....biology circuits. What is a molecular biologist to do? Let’s take a look at how plasmids recently deposited...JChristopher Anderson. "Rapid optimization of gene dosage in E. coli using DIAL strains." Journal of biological... -
Why and how to start studying chromatin (with Addgene's help)
TypeBlog PostPublishedJune 12, 2018, 11:40 a.m.... posts are not comprehensive, so just because you do not see a name or gene on this list, that does not... -
Visualizing Protein Turnover In Situ
TypeBlog PostPublishedJan. 16, 2018, 3:20 p.m....blogger, Eugenia Rojas. A question worthy of a PhD: How do you visualize protein turnover within a neuron? For... -
Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity
TypeBlog PostPublishedNov. 5, 2019, 1:28 p.m....we first published a blog post titled: Which Cas9 Do I Choose for My CRISPR Experiment? The three years... -
It’s Bioelectric! An Exciting Interdisciplinary Field of Research
TypeBlog PostPublishedOct. 29, 2019, 2:52 p.m....communication is not just for neurons. Non-neuronal cells do this too. It's just that for non-neural cells, ion-based... -
Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection
TypeBlog PostPublishedJune 25, 2019, 12:54 p.m....interest into a bacterial cell or a host’s genome. To do this scientists commonly use phagemids, a DNA cloning... -
FlipGFP, a novel fluorescence protease reporter to study apoptosis
TypeBlog PostPublishedMay 21, 2019, 12:10 p.m....spatio-temporal dynamics of apoptosis in living animals. To do this they modified a different self-assembling split... -
A Quick Guide to a Career in Software Product Management
TypeBlog PostPublishedNov. 24, 2020, 2:15 p.m....go to grad school. But, I didn’t know what else to do. Fortunately, the head of software product management... -
Summer SciComm: Preprints
TypeBlog PostPublishedAug. 16, 2022, 1:15 p.m....broader community, and preprints are a great way to do that. We are happy to receive deposits of plasmids... -
CasPEDIA: A Functional Classification of Cas Enzymes
TypeBlog PostPublishedJan. 25, 2024, 2:15 p.m....resources on the Addgene blog CRISPR 101: Which Cas9 do I choose for my CRISPR experiment? CRISPR 101: ... -
Viral Vectors 101: Viral Vector Elements
TypeBlog PostPublishedJuly 25, 2023, 1 p.m....and are slightly different in sequence. Both LTRs do share common repeat regions in addition to their ...virion assembly and particle release from late endosomes when AAV is trafficked. Rep and cap mediate AAV... -
All in a Twist: dsRNA
TypeBlog PostPublishedFeb. 2, 2023, 2:15 p.m....produced dsRNA rarely reach this threshold and thus do not typically activate an inflammatory response. ... -
Antibodies 101: Polyclonal Antibodies
TypeBlog PostPublishedJuly 14, 2021, 1:15 p.m....strength of staining and background noise. If you do use different lots of your polyclonal antibody, you... -
Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics
TypeBlog PostPublishedMarch 3, 2020, 2:15 p.m....sensors in terms of response and brightness. They also do not spectrally separate from green sensors and blue-light-activated.... The dosing required to achieve these efficiencies was also between 1013 - 1014 vg/kg, a dosage comparable... -
Addgene's Tips for Plasmid Quality Control
TypeBlog PostPublishedJan. 14, 2016, 3:30 p.m....sequences to visually check for homology and mismatches. Do you have your own best sequencing practices or maybe...