We narrowed to 553 results for: ESC
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TypeBlog PostPublishedMay 6, 2025, 1:15 p.m....types of data for each of their plasmids, from descriptions of key components of the plasmids to partial...
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Design Tips for Prime Editing
TypeBlog PostPublishedJan. 23, 2025, 2:15 p.m....but this also allows certain unintended edits to escape cellular surveillance. To limit unintended incorporation... -
A Look Back at One Year of Plasmid Sharing for COVID-19 Research
TypeBlog PostPublishedJuly 12, 2021, 1:15 p.m....the repertoire of antibodies from 19 COVID-19 convalescent individuals (Tong et al., 2021). Another version... -
Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene
TypeBlog PostPublishedApril 6, 2021, 12:30 p.m.... use a modified version of the Python program described by Joung et al. 2017, which we hope to make available... -
Tips for Technical Support Calls
TypeBlog PostPublishedSept. 20, 2016, 2:39 p.m....plasmids and pooled libraries were originally described in a peer-reviewed scientific publication. We ... -
Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux
TypeBlog PostPublishedSept. 13, 2016, 2:30 p.m....driglar. References: 1. Alberts, Bruce, et al. "Rescuing US biomedical research from its systemic flaws... -
Adapting Toehold Switches to Detect Zika Virus
TypeBlog PostPublishedAug. 30, 2016, 2:30 p.m....ability to complete the project on such a short timescale. Our article ultimately reported the development... -
Cpf1 Update: Comparison to Cas9 and NgAgo
TypeBlog PostPublishedJuly 14, 2016, 2:30 p.m....for Cas9 (42 nt vs 100 nt.) However, the newly described non-CRISPR protein NgAgo is smaller than any of... -
Tips for Titering Your Lentiviral Preps
TypeBlog PostPublishedMarch 15, 2016, 2:30 p.m....is particularly useful when vectors carrying fluorescent markers are used as it eliminates the need for... -
7 Simple Strategies to Resolve Conflicts with Difficult Supervisors
TypeBlog PostPublishedJan. 21, 2015, 1:30 p.m....familiar with the minute details of your work. Describe the problem by stating the facts and explain how... -
How-to: 5 Steps to a Great Panel Discussion
TypeBlog PostPublishedOct. 22, 2014, 1:57 p.m....organizations, I asked audience members to stand and describe unique nonprofit models that differed from the... -
Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity
TypeBlog PostPublishedOct. 11, 2018, 12:30 p.m.... assemblies per plate using outgrowth volumes described in Figure 3, with calculated yields from entire... -
CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments
TypeBlog PostPublishedFeb. 7, 2019, 2:16 p.m....protein coding genes in the human genome with fluorescent proteins. For each gene, we needed to design ... -
3D Printing Meets CRISPR Cas9
TypeBlog PostPublishedOct. 17, 2017, 12:55 p.m....reaction to this model on his blog. Or, for a description of how effective this model was in explaining... -
AAV Vector Quality Control: Going the Extra Mile with NGS
TypeBlog PostPublishedSept. 12, 2017, 1:44 p.m....transduced cells whenever possible. This post will describe our workflow for viral genome sequencing (VGS)... -
Quick Guide to Working with Drosophila Part 1: Getting Started with Flies
TypeBlog PostPublishedJuly 13, 2017, 2:30 p.m....(the Gal4/UAS system), and the third post will describe how you can make your own mutant flies. Find Drosophila... -
Of Myc and Men
TypeBlog PostPublishedJan. 19, 2023, 2:15 p.m....that perfect. One of the known limitations was described by Schüchner, et al., who published a paper showing... -
Academic vs. Industry Postdocs
TypeBlog PostPublishedNov. 13, 2018, 1:22 p.m....academia, however, writing papers and grants is an inescapable and essential part of being able to continue ... -
Addgene's Cancer Collection Pages Connect the Research Community
TypeBlog PostPublishedSept. 6, 2016, 2:30 p.m....through several different applications such as fluorescent protein fusion studies and the generation of ... -
Genome-wide Screening Using CRISPR
TypeBlog PostPublishedAug. 18, 2015, 2:30 p.m.... are enriched using either drug selection or fluorescence-based cell sorting and screened for a particular...