We narrowed to 530 results for: Erf

1. How to Be an Excellent Trainee

   Type

   Blog Post

   Published

   Sept. 26, 2023, 1:15 p.m.

   ...enough information there for you to be independent performing the technique you trained to do? Walk through...

2. QC Sequencing Technologies at Addgene

   Type

   Blog Post

   Published

   July 15, 2025, 1:15 p.m.

   ...specified discrepancies. Any sequences that are a perfect match from this script can then continue in the...

3. Twenty Years of Sharing: Addgene's Viral Vector Service

   Type

   Blog Post

   Published

   July 30, 2024, 1:15 p.m.

   ...vector was useful for the requestors or how it performed. Addgene launched our AAV Data Hub as a community...

4. Hot Plasmids Spring 2024

   Type

   Blog Post

   Published

   May 23, 2024, 1:15 p.m.

   ...Ace2N-mNeon also led to similar improvements in performance.      Figure 4: Structure of Voltron...

5. Tips for Writing a Good Cover Letter

   Type

   Blog Post

   Published

   May 21, 2024, 1:30 p.m.

   ...for Scientists Series Get Tips on Finding Your Perfect Job after Graduation Additional Resources Hamilton...

6. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

   Type

   Blog Post

   Published

   Jan. 24, 2023, 2:15 p.m.

   ...dilute the unintegrated plasmids (Fig. 2b). Next, perform a short (12-hour) induction with doxycycline prior...

7. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

   Type

   Blog Post

   Published

   Feb. 18, 2020, 2:15 p.m.

   ...conference, Coastal & Estuarine Research Federation (CERF) Biennial Conference, Gordon Research Conference...

8. CRISPR-based Genome Editing Systems For Editing in E. coli

   Type

   Blog Post

   Published

   April 13, 2021, 1:15 p.m.

   ...strand breaks it introduces makes it difficult to perform two or more integrations on the genome. Most recently...

9. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

   Type

   Blog Post

   Published

   Nov. 1, 2018, 12:35 p.m.

   ...discovery, plasmid addiction systems have become a powerful tool to address a diverse set of biological inquiries...

10. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

    Type

    Blog Post

    Published

    Dec. 14, 2017, 2:08 p.m.

    ...difficult to obtain. These can then be used to perform cell therapy and to model diseases in vitro. Find...

11. Using Video to Share Your Science: We Share Science

    Type

    Blog Post

    Published

    Nov. 3, 2016, 2:30 p.m.

    ... for diagnosing and resolving organizational performance and often have to be able to quickly synthesize...

12. 5 Reasons to Use Reddit for Science Communication

    Type

    Blog Post

    Published

    Oct. 20, 2016, 2:30 p.m.

    ...publically about your work (you can find my tips for a perfect science talk here). Finally, my main content organizer...

13. Tips for CRISPR Gene Editing in Mice

    Type

    Blog Post

    Published

    June 28, 2016, 10:59 a.m.

    ...generate your pCAG-EGXXFP plasmid? They are the perfect primer sets to use to genotype your pups. A simple...

14. The Challenges of Cell Culture

    Type

    Blog Post

    Published

    May 5, 2016, 2:30 p.m.

    ...making it easier for comparable studies to be performed on a cell line even decades after it is initially...

15. Getting the Most from Your Lentiviral Transduction

    Type

    Blog Post

    Published

    April 7, 2016, 2:30 p.m.

    ...Lentiviruses are a powerful laboratory tool often employed to establish cell lines that stably express...

16. Important Considerations in Optogenetics Behavioral Experiments

    Type

    Blog Post

    Published

    Oct. 1, 2015, 2:30 p.m.

    ...in the laboratory mouse, most optogenetics is performed in this species. However, transgenic rat technology...

17. Plasmids 101: E. coli Strains for Protein Expression

    Type

    Blog Post

    Published

    Feb. 10, 2015, 3:06 p.m.

    ... expression from high-copy number plasmids and powerful promoters will greatly exceed that of any native...

18. Plasmids 101: The Promoter Region – Let's Go!

    Type

    Blog Post

    Published

    April 3, 2014, 8:05 p.m.

    ...a good place to start when trying to pick your perfect promoter! Eukaryotic Promoters   Promoter   ...

19. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type

    Blog Post

    Published

    Feb. 22, 2017, 3:30 p.m.

    ...ease of use, 2) time savings, and 3) ability to perform single site or multi-site saturation mutagenesis...

20. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type

    Blog Post

    Published

    July 10, 2018, 1:08 p.m.

    ...notes etc. Detailed records about every action performed in the lab (user, date, time, action) are required...