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We narrowed to 809 results for: LAS

Showing: 481 - 500 of 809 results

  1. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    Published
    June 28, 2018, 3:01 p.m.
    ...on the Addgene Blog Plasmids for endogenous gene tagging in human cells Plasmids 101: Protein Tags Fluorescent...from many pitfalls: they require construction of plasmid vectors and/or rely on cumbersome in vitro synthesis...directly purchased and there is no need to construct plasmid vectors or synthesize/purify IVT sgRNA in the lab...SapTrap Resources on Addgene.org Browse CRISPR plasmids Check out our CRISPR guide pages Find ready-to-use...

  2. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    Published
    March 14, 2017, 2:30 p.m.
    ...inflammatory responses can augment tumor growth. Indeed, lasting responses to viral infections have resulted in ...expression of the major histocompatibility complex (MHC) class I proteins on their surfaces to avoid recognition... However, these conventional therapies produce lasting side effects, damage healthy cells, and often cannot...expressing CD19 chimeric antigen receptors for acute lymphoblastic leukaemia in children and young adults: a phase... Blog Learn About Our Cancer Collection Pages Plasmid Grant to Aid Cervical Cancer Screening in Ghana...

  3. Editor's Choice, September 2016

    Type
    Blog Post
    Published
    Oct. 7, 2016, 4 p.m.
    ...Pharmaceuticals. This post highlights a rare disease, Fibrodysplasia ossificans progressiva (a.k.a. FOP), that, ... Tips for Writing a Good Cover Letter Multiple Plasmids at a Low Price: Kits from Addgene Savvy Advocates...Kinome Libraries Available: Pooled and Individual Plasmid Formats Better Dyeing Through Chemistry & Small...Addgene supporters who spoke with me and deposited plasmids on this trip. Here’s to hoping that October is...

  4. Editor's Choice, August 2016

    Type
    Blog Post
    Published
    Sept. 2, 2016, 1:32 p.m.
    ...August - at over 56,000 views, we’ve grown 12% over last month! This was a bit surprising given how many ...the excellent content they’ve churned out in the last month. Google Forums Round Up: First Impressions... can use PCR to make small sequence changes to plasmid DNA. This an oldie but a goodie, and in my personal...experience, is a quick way to generate many mutated plasmid constructs. The second post was contributed by ...

  5. From our Table to Yours: An Inside Look at Lunch at Addgene

    Type
    Blog Post
    Published
    Nov. 27, 2013, 2:54 p.m.
    ...features that ingredient. We just had a cook-off last week, and the featured ingredient was pumpkin. Everyone... from cleaning out the freezers to writing new plasmid protocols for our website. Free lunch is provided... labs, and people work hard at Addgene to make plasmid sharing as easy as possible. However, I think it...keeps us smiling even when we’re packing the 400th plasmid that day.   As we head into Thanksgiving weekend...

  6. Same Addgene, New Look - Why We Redesigned Our Homepage & Mascot

    Type
    Blog Post
    Published
    Dec. 5, 2019, 1:49 p.m.
    ...while and hadn’t kept pace with our growth over the last few years. Some of the navigation was buried on ...look clean and easy to use.  Easily search for plasmids (and more!) The main goal for the homepage was...functionality. We wanted users to be able to find plasmids and viral vector tools more easily. In the redesign...hope every time you come to Addgene to find the plasmid or viral vector for your next experiment, that ...

  7. Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening

    Type
    Blog Post
    Published
    Feb. 20, 2014, 4:57 p.m.
    ... smaller size of the plasmids. The Zhang lab, on the other hand, uses a 1 plasmid, single infection system... thousands of targets at once. That’s what our plasmids allow.” Wang’s library is designed to target a...Library. Koike-Yusa H, Li Y, Tan E-P, Del Castillo Velasco-Herrera M, Yusa K. Nature Biotechnology. 2013 Dec...

  8. Cellosaurus: A Cell Line Information Database

    Type
    Blog Post
    Published
    Feb. 21, 2023, 2:15 p.m.
    ...tool (CLASTR) to find cells lines similar to your STR profiles of interest. You can filter CLASTR results...Cellosaurus, and CLASTR, to finally know whether it was an 'h' or a 'b' written on the flask (and if the name...

  9. Bricking Science: Portraying Scientific Reality Through LEGO

    Type
    Blog Post
    Published
    Nov. 29, 2016, 3:30 p.m.
    ...laboratory sets so we had to purchase the big LEGO classic box, the Big Bang Theory set, and then use our ... process was to embed personality in pieces of plastic, but it’s gotten easier now that I identify with...the main character is much more than a piece of plastic for us now). With the personality in place, everything...Find Protocols and Protocol Videos Check out LeGO plasmids from the Fehse Lab ...

  10. Walking beside Academia and giving it a high five: My transition from graduate work to a position at Addgene

    Type
    Blog Post
    Published
    July 2, 2015, 8:37 p.m.
    ...coli to produce biofuels in Pamela Silver’s Lab (plasmid page here). Now I spend much less time wrangling...helping scientists share information and, of course, plasmids. My primary duties are to manage this very blog...interesting than that will simply get people ordering plasmids. The need to keep content coming also keeps me...talking to researchers about their work, picking up plasmid deposits, and trying to learn ways Addgene can ...

  11. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

    Type
    Blog Post
    Published
    Jan. 24, 2019, 2:24 p.m.
    ...The principle of Tetbow In modern neuroscience, plasmid or viral vector-mediated gene delivery is more ... use Tetbow, you can introduce the four Tetbow plasmids (Addgene #104102 - #104105) using in utero electroporation...Tips and troubleshooting your Tetbow experiment Plasmid concentrations and AAV titer - The XFP genes have...achieve the highest color variations. Adjust the plasmid concentrations or AAV titer for the best results...

  12. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    Published
    Aug. 31, 2023, 1 p.m.
    ... BRAIN initiative plasmid collection More resources on the Addgene blog Plasmid tools for microbiome...Anikeeva lab are microelectronic fibers made from thermoplastic polymers that can work with all the bells and...authors optigenetically and chemically excited a class of enteroendocrine cells within the intestines known...

  13. Safe Port in a Storm...How Addgene is Weathering the Pandemic

    Type
    Blog Post
    Published
    Sept. 17, 2020, 1:15 p.m.
    ...urgent requests for access to coronavirus-related plasmids and to receive important coronavirus-related deposits...shipping staff agreed to come in and start getting plasmid deposits into the collection and to start shipping...support Addgene now, when their distribution of plasmids for COVID-19 research is accelerating essential...-related slowdown. More scientists are sharing plasmids and other reagents every day, and this crisis ...

  14. HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

    Type
    Blog Post
    Published
    Oct. 10, 2019, 12:41 p.m.
    ...protein guide Find plasmids for subcellular localization Find fluorescent protein plasmids ...signal to noise ratio five fold over the 1xHA tag.  Lastly, the researchers found that the HA frankenbody ...the ability to bind nascent HA tagged proteins.  Lastly, the HA frankenbody can be used for imaging in ...

  15. Another Pathway into Cells: iTOP

    Type
    Blog Post
    Published
    June 23, 2015, 8:37 p.m.
    ...at least four times more efficient in primary fibroblasts. iTOP also works in multiple types of primary...and gut stem cells, dendritic cells, embryonic fibroblasts, glia cells and neurons, and cell death post-... which transient protein expression has a long-lasting effect, such as Cre- or Cas9-mediated recombination...10.1016/j.cell.2015.03.028. PubMed. Find the Plasmids @Addgene: iTOP-tested proteins and reporters...

  16. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    Published
    March 19, 2020, 1:15 p.m.
    ...have yoga class twice a week at Addgene HQ, but now we’re sharing links to online yoga classes. Get outdoors...recently? Most of us at Addgene began working from home last week to flatten the curve, but working remotely ... Many studios and gyms are now offering online classes and participating in these is a great way to support...

  17. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    Published
    Jan. 12, 2021, 2:15 p.m.
    ... waste of our time.  Networking connections can last years This is a graphic snapshot of my LinkedIn ...graduate school, there was only one other person in my class who had children. When I had my baby, we became ...the postdoc association. It was 25 years since our last contact, but we were delighted to be reconnected...years in many different ways. This leads me to my last tip: It’s usually not worth connecting with people...

  18. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    Published
    Aug. 23, 2022, 1:15 p.m.
    ...shyer classmates. For my undergraduate degree, I majored in human anatomy, but I also took classes in biochemistry...but adamantly refused to get them vaccinated. A classmate told me that they never finish a course of antibiotics...anxious, whispered questions from friends and classmates back in high school, and it’s oddly fitting that...

  19. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type
    Blog Post
    Published
    June 1, 2017, 2:30 p.m.
    ...mammalian cells is transiently transfected with a plasmid containing a fluorescent protein (fusion) there...dish. This is caused by the stochastic nature of plasmid uptake by cells. To correct for this variation,...Goedhart et al, 2012, Bindels et al, 2017). The plasmid for co-expressing mTurquoise2 with mVenus as a ...Find Empty Backbones with Fluorescent Tags Find Plasmids for Subcellular Localization Browse the entire...

  20. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    Published
    Feb. 21, 2019, 1:15 p.m.
    ...specific mutations in your plasmids Use REPLACER Mutagenesis to mutate your plasmid Resources on Addgene.... targeted mutagenesis experiments? Find EvolvR plasmids at Addgene! References Gaj, Thomas, and Pablo...Browse our CRISPR guide pages Find bacterial CRISPR plasmids ...
Showing: 481 - 500 of 809 results