We narrowed to 821 results for: LAS

...recombinant antibodies (rAbs), which are produced from plasmids and have very little batch-to-batch variation.... of sequence can be done from relatively cheap plasmid sequencing. While rAbs are monoclonal, recombinant... buffer than proteins easily available in the cytoplasm or whole-cell lysate. A harsher, SDS-containing...However, these systems are expensive, generate plastic waste, and limit the amount of optimization that...temperature for 1–2 years and are available in formulas specific to your choice of detection methods. ...antibody required. Reporters There are two main classes of reporters: chemiluminescence enzymes and fluorescent...

...Addgene.org Find Fluorescent Protein Plasmids Find Plasmids for FRET Find Plasmids for Subcellular Localization...on Choosing the B(right)est Fluorescent Protein Plasmids 101: Visualizing Subcellular Structures and Organelles...

...Addgene.org Find Fluorescent Protein Plasmids Find Plasmids for FRET Find Plasmids for Subcellular Localization...organization (now with more than 50% international plasmid distribution!) Chapter 8 - Opportunities to create...

...can request any of over 3,000 eligible AAV cargo plasmids in the Addgene repository to be packaged in your...Request With Packaged on Request, you can find your plasmids and your viral packaging service in the same place...packaged on request process, simply navigate to your plasmid of interest. If it’s eligible for packaged on request...special processes involved; simply request your plasmid of interest in your desired serotype, place your...

... there? A typical biomedical postdoc fellowship lasts five years. Most universities and institutes annually...feel like they've made enough progress since the last presentation. Yet, PFs need to tell and sell their... practice. If you simply recycle slides from the last time you gave a presentation to a specific group...trainees, communicate your expectations, and teach classes. Therefore, seek out management and conflict resolution...quality control of secretory proteins in the Endoplasmic Reticulum, optimization of fluorescent proteins...

...Blog Learn New Plasmid Cloning Techniques Pushing the Limits of DNA Assembly Plasmid Cloning by PCR ...including vector-insert joining for recombinant plasmid construction (restriction cloning), adaptor ligation...resistance-mediating polymorphisms in African samples of Plasmodium falciparum." Journal of clinical microbiology...Jayakrishnan, Pravin A. Nair, and Stewart Shuman. "Last stop on the road to repair: structure of E. coli...

...Last week, 20 students and four chaperones from Denis-Yarmouth Regional High School visited our labs ...some of the teams at Addgene. An introduction to plasmids with Director of Biology Lianna Swanson was very...real working lab full of busy Addgenies preparing plasmid samples for shipping. This was the favorite part... all over the world know that we can help with plasmid sharing! Judging by how many prizes were given ...

...exhibitor booth in order to help scientists find the plasmids they need for their research, show labs how they...about all the amazing research being done with plasmids. I heard an impressive talk about the use of optogenetics...and resources by storing and distributing their plasmids with the scientific community. All of this speaks... meet our Addgenie crew and sign-up for a free plasmid! Also check out the PLOS Neuroscience Community...

...s incredible.” Looking for CRISPR Plasmids? CRISPR-Cas9 Plasmids available at Addgene CRISPR-Cas9 References...important advance was quickly followed by even splashier news: Jiahao Sha of Nanjing Medical University...understanding of how CRISPR/Cas9s actually work. Last month, the Lawrence Berkeley Lab's Jennifer Doudna...

...Last updated Apr 17, 2020. Before being adapted by scientists to edit the genome of virtually any organisms...size of other Cas proteins found in the so-called class 2 CRISPR-Cas systems. There are 24 variants of the...regions containing the putative tracrRNA into a plasmid and expressed it in an E. coli bacteria strain....sheet Resources on Addgene.org Find the Cas14 plasmids here Checkout our CRISPR guide page Browse the...

...on the Addgene Blog Plasmids for endogenous gene tagging in human cells Plasmids 101: Protein Tags Fluorescent...from many pitfalls: they require construction of plasmid vectors and/or rely on cumbersome in vitro synthesis...directly purchased and there is no need to construct plasmid vectors or synthesize/purify IVT sgRNA in the lab...SapTrap Resources on Addgene.org Browse CRISPR plasmids Check out our CRISPR guide pages Find ready-to-use...

...inflammatory responses can augment tumor growth. Indeed, lasting responses to viral infections have resulted in ...expression of the major histocompatibility complex (MHC) class I proteins on their surfaces to avoid recognition... However, these conventional therapies produce lasting side effects, damage healthy cells, and often cannot...expressing CD19 chimeric antigen receptors for acute lymphoblastic leukaemia in children and young adults: a phase... Blog Learn About Our Cancer Collection Pages Plasmid Grant to Aid Cervical Cancer Screening in Ghana...

...Pharmaceuticals. This post highlights a rare disease, Fibrodysplasia ossificans progressiva (a.k.a. FOP), that, ... Tips for Writing a Good Cover Letter Multiple Plasmids at a Low Price: Kits from Addgene Savvy Advocates...Kinome Libraries Available: Pooled and Individual Plasmid Formats Better Dyeing Through Chemistry & Small...Addgene supporters who spoke with me and deposited plasmids on this trip. Here’s to hoping that October is...

...August - at over 56,000 views, we’ve grown 12% over last month! This was a bit surprising given how many ...the excellent content they’ve churned out in the last month. Google Forums Round Up: First Impressions... can use PCR to make small sequence changes to plasmid DNA. This an oldie but a goodie, and in my personal...experience, is a quick way to generate many mutated plasmid constructs. The second post was contributed by ...

...features that ingredient. We just had a cook-off last week, and the featured ingredient was pumpkin. Everyone... from cleaning out the freezers to writing new plasmid protocols for our website. Free lunch is provided... labs, and people work hard at Addgene to make plasmid sharing as easy as possible. However, I think it...keeps us smiling even when we’re packing the 400th plasmid that day.   As we head into Thanksgiving weekend...

...while and hadn’t kept pace with our growth over the last few years. Some of the navigation was buried on ...look clean and easy to use.  Easily search for plasmids (and more!) The main goal for the homepage was...functionality. We wanted users to be able to find plasmids and viral vector tools more easily. In the redesign...hope every time you come to Addgene to find the plasmid or viral vector for your next experiment, that ...

... smaller size of the plasmids. The Zhang lab, on the other hand, uses a 1 plasmid, single infection system... thousands of targets at once. That’s what our plasmids allow.” Wang’s library is designed to target a...Library. Koike-Yusa H, Li Y, Tan E-P, Del Castillo Velasco-Herrera M, Yusa K. Nature Biotechnology. 2013 Dec...

...tool (CLASTR) to find cells lines similar to your STR profiles of interest. You can filter CLASTR results...Cellosaurus, and CLASTR, to finally know whether it was an 'h' or a 'b' written on the flask (and if the name...

...laboratory sets so we had to purchase the big LEGO classic box, the Big Bang Theory set, and then use our ... process was to embed personality in pieces of plastic, but it’s gotten easier now that I identify with...the main character is much more than a piece of plastic for us now). With the personality in place, everything...Find Protocols and Protocol Videos Check out LeGO plasmids from the Fehse Lab ...

...coli to produce biofuels in Pamela Silver’s Lab (plasmid page here). Now I spend much less time wrangling...helping scientists share information and, of course, plasmids. My primary duties are to manage this very blog...interesting than that will simply get people ordering plasmids. The need to keep content coming also keeps me...talking to researchers about their work, picking up plasmid deposits, and trying to learn ways Addgene can ...