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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 751 results for: Nes;

Showing: 481 - 500 of 751 results

  1. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type
    Blog Post
    Published
    April 25, 2019, 3:01 p.m.
    ...was then screened through successive rounds of mutagenesis for the brightest teal fluorescent proteins that...scientists from Florida State University, they used a mutagenesis approach and found that specific amino acid changes...

  2. New and Upcoming Viral Vectors - Spring 2019

    Type
    Blog Post
    Published
    March 26, 2019, 1:40 p.m.
    ...with different properties (for example, overall brightness or on/off kinetics) that makes them suitable ...pAAV.hSyn-FLEX.iGABASnFR) has good membrane localization and brightness. The F102G variant (pAAV.hSyn-FLEX.iGABASnFR....

  3. Viral Vectors 101: Transductions

    Type
    Blog Post
    Published
    April 30, 2024, 1:15 p.m.
    ...transduction methods. Some ‘difficult to transfect’ cells lines are amenable to transduction, making it a logical...Resources on Addgene.org Generating Stable Cell Lines with Lentivirus Virus Handling and Storage Addgene...

  4. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    Published
    June 9, 2016, 2:30 p.m.
    ... and genomic DNA in a variety of mammalian cell lines, with an efficiency similar to that of Cas9. Like...certain guide sequences - using 5 guides/gene for 10 genes, they did not observe differences in cleavage efficiency...

  6. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    Published
    Feb. 13, 2024, 2:15 p.m.
    ...performed using traditional 2D assays with immune cell lines or peripheral blood mononuclear cells (PBMC) in .... P., Herskovitz, J., Goletz, T. J., Zhou, L., Moxness, M., Flynn, G. C., Narhi, L. O., & Jawa, V. (2016...

  7. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    Published
    Aug. 22, 2023, 1 p.m.
    ... know that science usually doesn’t follow our timelines! Prepare a research plan to set yourself up for...applying to a structured program, they likely have guidelines to consult on this topic.   Etiquette...

  8. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    Published
    April 4, 2023, 1 p.m.
    ...experimental model. In fact, approximately 25% of yeast genes have a human counterpart, enabling study of human-relevant...the strain tractable for peroxisomal studies on biogenesis, degradation, and proliferation. Genome manipulation...

  9. Cultivating Community Science at BosLab

    Type
    Blog Post
    Published
    Nov. 2, 2017, 1:35 p.m.
    ... with audiovisual media, software engineering, business consulting, mathematics, and more. That’s right...BosLab. Once he optimizes plant cell culture and transgenesis he wants to share his knowledge with the community...

  11. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    Published
    March 19, 2020, 1:15 p.m.
    ... help you prepare for sleep. At Addgene, our mindfulness group is sticking with their Headspace practice...19. You can also give back by supporting local businesses (ex: purchasing a gift card to use later or ordering...

  13. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    Published
    April 18, 2019, 12:53 p.m.
    ...the gRNA target site. In addition to increased brightness, they also found that the signal to noise ratio... observing low-copy genomic loci or single copy genes. If you want to visualize genomic loci brightly,...

  14. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    Published
    Nov. 7, 2019, 2:18 p.m.
    ...going forward. This is not just a question of being honest and straightforward, it’s an important part of ... various different versions of checklists and guidelines to help authors understand what they are expected...

  15. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    Published
    Jan. 12, 2021, 2:15 p.m.
    ...my guess is that people will not be exchanging business cards for awhile or ever so your LinkedIn profile...She supported me and answered my questions and honestly, she was the only person who really understood...

  16. 5 Great Apps for Lab Life

    Type
    Blog Post
    Published
    Sept. 22, 2016, 2:30 p.m.
    ...smartphone to help me with my research in terms of preparedness, organization, and sharing data. Here are five...Stacey! Sean D. Stacey is a Ph.D. student at East Tennessee State University. He works with gene expression...

  17. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    Published
    March 24, 2016, 2:30 p.m.
    ...disrupt target gene sequences (thus inactivating genes), while breaks repaired by homology directed repair... which sequence replacement holds some kind of fitness advantage but error-prone repair of the gene would...

  18. Components of CRISPR/Cas9

    Type
    Blog Post
    Published
    Feb. 2, 2016, 5 p.m.
    ...Variant of Cas9 PAM Sequence Streptococcus pyogenes (SP); SpCas9 3' NGG SpCas9 D1135E variant...sites with the aid of two RNAs: the crRNA which defines the genomic target for Cas9, and the tracrRNA which...

  19. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    Published
    June 15, 2023, 1:15 p.m.
    ...focus on development, along with the inherent trickiness of using and optimizing GCE, led to a large toolbox...essentially those needed to make site-directed mutagenesis, many researchers new to GCE aren’t able to successfully...

  20. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    Published
    Feb. 28, 2023, 2:15 p.m.
    ...microscope has been a powerful tool in almost all disciplines. In biology, innovative new imaging approaches...shows an image of differentiating monocyte with planes and hardware to it. Right panel shows workflow ...
Showing: 481 - 500 of 751 results