We narrowed to 552 results for: PPL;

1. Gaining Leadership Skills Volunteering at a Professional Organization

   Type

   Blog Post

   Published

   Aug. 6, 2015, 2:30 p.m.

   ...institutions are not local but are national issues. I applied and was successfully granted a travel award to ...

2. Degrading DNA with Cascade-Cas3

   Type

   Blog Post

   Published

   Feb. 11, 2025, 2:15 p.m.

   ...Mashimo, T. (2022). Genome editing technology and applications with the type I CRISPR system. Gene and Genome...

3. Pooled Library Amplifications

   Type

   Blog Post

   Published

   May 30, 2024, 1:30 p.m.

   ...to amplify the sample for further downstream applications. As individual libraries can vary significantly...

4. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

   Type

   Blog Post

   Published

   April 4, 2019, 12:53 p.m.

   ...single-gene knockdown experiments. Mobile-CRISPRi applications Mobile-CRISPRi expands CRISPRi to non-model ...

5. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

   Type

   Blog Post

   Published

   Oct. 30, 2018, 1:04 p.m.

   ...Pluripotent Stem Cells: Problems and Advantages when Applying them in Regenerative Medicine. Acta Naturae, 2010...

6. 10 Basic tips for mammalian cell culture

   Type

   Blog Post

   Published

   July 12, 2018, 1:09 p.m.

   ...expression disregulation on HeLa cells." Journal of applied phycology27.6 (2015): 2321-2332. 3. Contamination...

7. xCas9: Engineering a CRISPR Variant with PAM Flexibility

   Type

   Blog Post

   Published

   March 28, 2018, 6:52 p.m.

   ...editing rates. This PAM flexibility should expand applications that require very specific targeting, like homology...

8. Cloning Mammalian Cells with the Agarose Method

   Type

   Blog Post

   Published

   Sept. 7, 2017, 12:17 p.m.

   ...population of transfected clones. The technique is applicable to a wide variety of cell lines, from HEK to ...

9. Fluorescent Proteins 101: Fluorescent Protein Timers

   Type

   Blog Post

   Published

   May 4, 2017, 2:30 p.m.

   ...-FT-N1 pTRE-Slow-FT pBAD/HisB-Slow-FT -  Applications of fluorescent protein timers FP timers have...

10. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type

    Blog Post

    Published

    Aug. 3, 2023, 1:15 p.m.

    ...order to correct for this, compensation has to be applied to “subtract” this spillover and correctly distinguish...

11. Of Myc and Men

    Type

    Blog Post

    Published

    Jan. 19, 2023, 2:15 p.m.

    ... flexible - it could now be used in way more applications.   Fig. 1 Making a Protein Tag (Abridged...

12. Transferable Skills: Negotiation

    Type

    Blog Post

    Published

    March 3, 2022, 2 p.m.

    ...mainly do over the price of a used car. Later, as I applied for jobs, I knew I should negotiate my salary after...

13. Addgene's Cancer Collection Pages Connect the Research Community

    Type

    Blog Post

    Published

    Sept. 6, 2016, 2:30 p.m.

    ...any of these genes through several different applications such as fluorescent protein fusion studies and...

14. PITChing MMEJ as an Alternative Route for Gene Editing

    Type

    Blog Post

    Published

    Feb. 23, 2016, 3:30 p.m.

    ...lies in the diversity of nucleases and their applications. It will be interesting to see what new editing...

15. Light Sheet Fluorescence Microscopy

    Type

    Blog Post

    Published

    July 16, 2015, 2:30 p.m.

    ...of LSFM microscopes (3,4). After this initial application, Stelzer’s group described the single-plane or...

16. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

    Type

    Blog Post

    Published

    Sept. 23, 2014, 3:05 p.m.

    ...plasmid with a downloadable protocol listed under “Supplemental Document”: gRNA_Cloning Vector (deposited by...

17. Which Fluorescent Protein Should I Use?

    Type

    Blog Post

    Published

    May 20, 2014, 2:06 p.m.

    .... al. (2010) “Fluorescent proteins and their applications in imaging living cells and tissues”  Physiol...

18. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type

    Blog Post

    Published

    Dec. 12, 2013, 3:35 p.m.

    ...substantial. It's estimated that genetic engineering as applied to products on the domestic market generates $300...

19. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type

    Blog Post

    Published

    Oct. 4, 2018, 12:44 p.m.

    ...Calabrese pooled library and lentiviral prep here! Applications for the Root and Doench CRISPR libraries Overall...

20. AAVs in Retinal Gene Therapy

    Type

    Blog Post

    Published

    July 31, 2018, 12:55 p.m.

    ...-1250015778 7. Yu W and Wu Z., 2018. In vivo Applications of CRISPR-Based Genome Editing in the Retina...