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We narrowed to 855 results for: REN

Showing: 481 - 500 of 855 results

  1. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    Published
    Jan. 6, 2017, 3:56 p.m.
    ...AAV8) enable different patterns of transduction and transgene expression across different cell types (.... Top AAV: pAAV-hSyn-DIO-hM4D(Gi)-mCherry Our current AAV inventory consists almost entirely of viruses...visualizing hM4D(Gi)-mCherry in fixed tissue. References 1. Aschauer DF, Kreuz S, Rumpel S. Analysis of...

  2. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    Published
    Feb. 25, 2014, 7:11 p.m.
    ...widely utilized across many different cell types and each one requires different elements for vector propagation...Instead, the size and A-T content of the DNA (apparently independent of a known specific sequence) dictate...auxotrophic markers and includes the associated references.  Yeast selection marker What does the marker...

  3. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type
    Blog Post
    Published
    Feb. 18, 2014, 4:22 p.m.
    ...formal mentoring program at my company, but there weren't enough senior people willing to step up and be ...hundreds of mentoring groups through my work with the renowned Healthcare Businesswomen’s Association (HBA) Boston...commitment of a formal mentoring relationship or there aren’t enough senior mentors available to go around. My...these criteria. If you can, include people from different labs, departments or organizations as it brings...

  4. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    Published
    Dec. 10, 2013, 3:13 p.m.
    ...stem cells edited to carry mutant versions of 15 different disease-associated genes. Addgene spoke to Musunuru...mutations. Once that's done in stem cells, we can differentiate those mutant and wild type stem cells into any...all have the mutations but they will also have different genetic backgrounds. It becomes more of an apples-to-oranges...suggest TALENs may be cleaner than CRISPR/Cas9. Reference: Quirong Ding et al. "A TALEN genome-editing system...

  5. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    Published
    April 20, 2023, 4 p.m.
    ...sixty seconds of silence–but it was thorough and coherent.  To be honest, if my paper hadn’t been ridiculously...to stop talking until I could present something coherent or to quickly string together all the words that...screeching feedback. You’re muted on Zoom. Your video conferencing software freezes and then crashes. You’re muted... a clear, informative presentation, speak at conferences, or even just provide quick updates during a ...

  6. To Each HIS Own

    Type
    Blog Post
    Published
    March 2, 2023, 2:15 p.m.
    ...if you need a very pure sample. Because binding strength is proportional to the length of the tag, with...Antibodies.) Unfortunately, for some proteins there just aren’t any good antibodies available. In these cases, ...antibody posts from our blog!  Resources and References  Resources on addgene.org Addgene Antibody Guide...ChIP Antibodies 101: Introduction to Antibodies References Hochuli E, Bannwarth W, Döbeli H, Gentz R and...

  7. RNA Extraction Without A Kit

    Type
    Blog Post
    Published
    March 24, 2020, 1:15 p.m.
    ...extra care when performing RNA extraction. RNA is inherently not as stable as DNA- it is single stranded and...ultracentrifuge.  Figure 1: An outline of the different steps in RNA extraction. Researchers at the... Reagent®, RNAzol®, QIAzol®  and sold by many different companies). TRIzol® is an all-in-one acid-guanidinium-phenol... ensure DNA contamination is not an issue.  References                 Allewell NM, Sama A (1974) The...

  8. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    Published
    Jan. 14, 2019, 2:08 p.m.
    ... vector team is publishing research, hosting conferences, and are expanding our catalog of materials to...appearance and functionality are happening and this strengthens our image in the world. Addgene is now as much...practices and benefits and these are making a difference. We serve scientists around the globe and our...Thanks to all the Addgenies and congratulations to current and alumni Addgenies on our success. Thanks to ...

  9. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    Published
    April 10, 2018, 12:56 p.m.
    ...antibodies Conventional antibodies are a non-renewable reagent - Their production requires animal sacrifice...produce - Hybridomas offer an alternative and renewable source of monoclonal antibodies, but they are ... their use. Each RANbody is detectable by two different tags: a reporter and an HA tag. The reporter comes... link to its sequence in the comments below. References Sanes, J.R., & Yamagata, M. (2018). Reporter-...

  10. Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute

    Type
    Blog Post
    Published
    Feb. 2, 2018, 3:20 p.m.
    ...SciComm by doing SciComm Writing had always been a strength of Susan’s, but she didn’t know if she liked science... of a team.” It took her two years to find her current position at Fred Hutch because her skill set wasn...connections she made through this program lead to her current job. “One of my colleagues in the class was like...Hutch magazine. Getting pulled in all of these different directions is sometimes at odds with the space...

  11. What's New in CRISPR - November 2021

    Type
    Blog Post
    Published
    Nov. 18, 2021, 2:15 p.m.
    ...mammalian systems has been in question. Three different labs have recently published work that provides... gene activation  and base editing, whereas a different set of mutations (the “V3.1” version) works best...miniature Cas system that utilizes a Cas12f from a different species, Acidibacillus sulfuroxidans, AsCas12f1...of up to 10kb but it is also capable of coding concurrent small insertions to allow in-frame deletions ...

  12. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    Published
    Oct. 20, 2022, 1:15 p.m.
    ...experiment moving. Happy editing! References and Resources References Hendel, A., Bak, R., Clark, J. et...talk about their precious RNA samples. RNA is inherently less stable than most macromolecules used in ...region of the gRNA. These gRNAs can be cleaved and rendered useless after less than a minute of exposure to...

  13. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    Published
    July 25, 2017, 1:20 p.m.
    ...but where the kinases are located, their signal strength, and how long their signals last all impact transduction... or “on.” Dronpa’s color thus indicates MEK1’s current state (Green when off, dark when on). Figure 1...function of the protein and pdDronpa are preserved. References 1. Zhou, Xin X., Linlin Z. Fan, Pengpeng Li, ...28232577. Figures 2-4 were modified from this reference. 2. Regot, Sergi, Jacob J. Hughey, Bryce T. Bajar...

  14. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

    Type
    Blog Post
    Published
    Dec. 16, 2015, 3:30 p.m.
    ...the target strand and the gRNA. Created with BioRender.com. To decrease the electropositivity of the...mutations). Although the precise mutations were different than those found in eSpCas9, the effect on specificity...and/or therapeutic settings. Will combining the different mutations found in each of these great tools further...continued improvements in genome engineering! References 1. Slaymaker, Ian M., et al. "Rationally engineered...

  15. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

    Type
    Blog Post
    Published
    May 5, 2015, 3:54 p.m.
    ...scientific manuscript will require numerous other references to scientific literature to substantiate the ...literature related to the topic. Finding reliable references and sorting out what they mean is no small task...focus in on a particular conclusion. Many papers aren’t always written this way and there is a greater ...inspiration and accountability. There are a few different ways to automate the process as well. Sign up ...

  16. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    Published
    Nov. 14, 2024, 2:15 p.m.
    ...without permanently altering the DNA, CRISPR interference (CRISPRi) and CRISPR activation (CRISPRa) are...study ncRNA, CRISPR systems offer a number of different options. By carefully considering your ncRNA of... you more about its function.  Resources and references Resources on addgene.org Addgene's CRISPR Guide... CRISPR enzymes for transcriptome engineering References Jinek M, Chylinski K, Fonfara I, Hauer M, Doudna...

  17. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    Published
    Aug. 29, 2024, 1:15 p.m.
    ...CRISPR experiment. Happy cloning! References and Resources References McGee, A. V., Liu, Y. V., Griffith...chosen because of their broad applicability in different model systems. What’s the error rate? The Doench...Table 1 has a list of Fragmid destination vectors currently available. Table 1: Fragmid destination vectors...

  18. Lab Automation at Addgene

    Type
    Blog Post
    Published
    March 22, 2018, 1:28 p.m.
    ... one. As a starting point, I would recommend conferences like Lab Robotic Interest Group (LRIG). There...customizability. It has a large deck that can house many different types of devices. As long as a device fits on ... Software for Biology Tips from the Repository Trenches: Using Barcodes to Track Samples Resources at...video library Check out our molecular biology reference pages ...

  20. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    Published
    Jan. 5, 2021, 2:15 p.m.
    ... recombinase/photodimer pairs. Created with BioRender.com In their recent ACS Synthetic Biology publication...fold more photostable and as bright to mVenus, the parent YFP, and is the most photostable YFP to date. As...and reduced RNA-off target footprint compared to current N-terminal linked SaCas9 ABE variants. It's also...specific tight affinity glutamate sensor from the Loren Looger Lab now available in AAV1. Get GFAP.SF-iGluSnFR.A184S-AAV1...
Showing: 481 - 500 of 855 results