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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 846 results for: RING;

Showing: 481 - 500 of 846 results

  1. Addgene Depositors Get More Citations

    Type
    Blog Post
    Published
    April 20, 2017, 2:30 p.m.
    .... While clearly an outlier, this story had us wondering: is there a larger trend here? Do papers associated...Management. She is particularly interested in exploring how innovative tools influence the direction of...References 1. Cong, Le, et al. "Multiplex genome engineering using CRISPR/Cas systems." Science 339.6121 (...

  2. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    Published
    Dec. 6, 2016, 3:30 p.m.
    ...the Wyss Institute for Biologically Inspired Engineering and member of the Addgene Advisory board, on ...aim to alleviate this frustration by producing, titering, testing, and providing lentiviral preps of select...the Wyss Institute for Biologically Inspired Engineering and the Zhang and Doench/Root labs from the Broad...

  3. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

    Type
    Blog Post
    Published
    July 21, 2020, 1:15 p.m.
    ...common ways to quantify DNA. This method involves measuring the absorbance/transmission of light through a...fluorometer to quantify DNA this way. First, start by pouring your gel containing a DNA intercalating dye (ex... the run time is just a few minutes per sample. During the run, DNA fragments move through a micro- or...

  4. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    Published
    Nov. 7, 2019, 2:18 p.m.
    ... and understand. All of this communication and sharing fits together to make for a transparent and collaborative... for data management plans and for evidence of sharing for grant renewal—but there is clearly room to ... all its different forms as part of graduation, hiring, funding, and promotion processes, I’m confident...

  5. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    Published
    Sept. 12, 2019, 1 p.m.
    ...from Janelia (jGCaMP7) continue to be useful for monitoring neuronal activity in the form of calcium dynamics...fluorescence and resting fluorescence (achieved through lowering sensor resting fluorescence, while maintaining...of our viral vectors blog posts Find tips for preparing AAVs Download our viral vectors 101 eBook Resources...

  6. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    Published
    June 18, 2019, 1:15 p.m.
    ...serotype for their particular study, which includes comparing the transduction patterns of different AAV serotypes...its calcium-bound state, which was achieved by lowering the basal fluorescence of the sensor. This “contrast...of our viral vectors blog posts Find tips for preparing AAVs Download our viral vectors 101 eBook Resources...

  7. The MTA at Addgene

    Type
    Blog Post
    Published
    July 23, 2024, 2:17 p.m.
    ...process. Even though this process is essential to sharing plasmids around the globe, it can be a bit of a...clear expectations from the beginning makes the sharing process smoother, more consistent, and more pleasant...MTA YouTube video for more information. Happy transferring!    Many thanks to our Tech Transfer team members...

  8. Hot Plasmids - January 2023

    Type
    Blog Post
    Published
    Jan. 31, 2023, 2:15 p.m.
    ...the weaponized T-cells (CAR-T cells) - and then delivering them to a tumor - they used focused ultrasound...staining, and light exposure can negatively affect FPs during these processes. The Liu and Petsko labs recently...only brilliantly bright, but it also withstands denaturing conditions, high temperatures, and other chaotropic...

  9. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    Published
    March 24, 2016, 2:30 p.m.
    ...pathways can modify the sequence of a gene (thus altering its function). HDR is crucial for certain applications...Moreover, the cost of reagents is relatively modest, requiring expressed Cas9 protein, transcribed sgRNA, and...al. "Enhanced homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery....

  11. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

    Type
    Blog Post
    Published
    Jan. 30, 2020, 2:15 p.m.
    ...these omissions, so it is unlikely to be caught during the peer review process, which often focuses more...respond as expected, use of representative images, measuring the main outcomes in several different ways, and... a particular result. Anything else that occurs during the course of the study, say a fifth time has unpredictably...

  12. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

    Type
    Blog Post
    Published
    June 25, 2019, 12:54 p.m.
    ...until it is triggered to enter the lytic cycle.   During both of these life cycles bacterial DNA can be ...in a wild type E. coli (Griffiths et al., 2000). During conjugation, genetic material is transferred from...Guillemin. "Multiple phases of competence occur during the Helicobacter pylori growth cycle." FEMS microbiology...

  13. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    Published
    Nov. 10, 2022, 2:15 p.m.
    ...approaches can be found. As an example, say you are considering either a cellular screen or a selection. A selection...scene, there are other options which are worth considering. RNAi screens are similar to CRISPR, but instead...temperature sensitive screens. These involve raising or lowering the culture temperature to exacerbate a mutant...

  14. Hot Plasmids - February 2022

    Type
    Blog Post
    Published
    Feb. 22, 2022, 2:15 p.m.
    ...creates challenges for in vivo delivery and cell engineering applications. CasMINI, a new miniature CRISPR-Cas... Figure 4: Overview of CasMINI for genome engineering applications. Image from Xiaoshu, et. al. 2021...system opens up new possibilities in both cell engineering and gene therapy.  Xiaoshu X, et al. Molecular...

  15. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type
    Blog Post
    Published
    Sept. 26, 2024, 1:15 p.m.
    ...ends to insert genes into plasmid backbones, base pairing between these ~4 base overhangs isn’t strong enough... be accessible to allow for complementary base pairing, so SLIC can’t be used if the overhangs would have...ligase! This post was originally written by Mary Gearing in December 2015 and was updated by Emily P. Bentley...

  16. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    Published
    Oct. 19, 2023, 1:15 p.m.
    ...an issue as they are thought to be self-repaired during the AAV replication process (Chen et al., 2004)... ITR integrity and prevent unwanted mutations?  During propagation, mutants rapidly replicate in a growth...help to reduce the likelihood of ITR deletions occurring. Check out our blog post for additional tips to...

  17. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    Published
    June 6, 2023, 1:15 p.m.
    ...do not require breakdown of the nuclear envelope during cell division to enter the nucleus as gamma-retroviruses...AAV also has multiple serotypes (12 naturally occurring and more synthetic capsids are routinely being...lentivirus, retrovirus, adenovirus, and AAV. If you’re wondering more about what applications these viruses are...

  18. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    Published
    June 2, 2015, 6:06 p.m.
    ...terminator. These relatively large cassettes (considering the gRNA itself is ~100 bases) limit the number...examined editing efficiency in intact rice plants, comparing traditional gRNA constructs with PTGs. In PTG ... Mary Gearing...

  19. How to Make Friends and Meet People at a Scientific Conference

    Type
    Blog Post
    Published
    Aug. 7, 2014, 1:58 p.m.
    ...of experimental problems or questions you are pondering and be ready to talk about them. People love to... to help especially when solving problems.  During the conference The conference may start in the airport...post. Stay in touch by liking, commenting and re-sharing their posts. LinkedIn users notice this sort of...

  20. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

    Type
    Blog Post
    Published
    July 10, 2014, 7:17 p.m.
    ...Sample6, for advice on how to take an idea developed during graduate school and turn it into a biotech company...changed, but Sample6 still leverages the core engineering and scientific concepts to engineer bacteriophages...co-founders raised $6 million. The biggest challenge during this time was learning to communicate effectively...
Showing: 481 - 500 of 846 results