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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 847 results for: STI;

Showing: 481 - 500 of 847 results

  1. What Do I Do Now? Academic v. Non-Academic Career Decisions

    Type
    Blog Post
    Published
    March 9, 2017, 3:40 p.m.
    ...to stay and good reasons to go.  Here are some questions and perspectives to consider. What do academic...have in common? You can do quality science on interesting topics Having strong non-science skills is important...important for success Must always justify use of resources and pay attention to budget constraints Academic...

  2. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    Published
    Nov. 18, 2016, 3 p.m.
    ...are incredibly useful, chemical fluorophores can still be used in a wide variety of experiments. Read more...and Alexander Green (both formerly at the Wyss Institute) to learn how these amazing tools may be used ...through Addgene.  Thanks again to all of our fantastic depositors and guest writers. Your contributions...

  3. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    Published
    April 7, 2015, 4:21 p.m.
    ...encode biosynthetic enzymes for violacein, a distinctive pigmented protein. By altering the transcriptional... By using an orthogonal dCas9 protein that will still recognize guide RNA but with a different dCas9 binding...of transcriptional programs can be layered with distinct programs controlled by each dCas9 regulatory switch...

  4. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    Published
    May 6, 2014, 1:07 p.m.
    ...Oliver Griesbeck of the Max Planck Institute for Neurobiology has been working on genetically encoded...these Twitch sensor variants were optimized by testing various linker configurations in a large-scale ... is disrupted in the presence of a ligand (they stick together in one state, but not the other). “It’s...

  5. Mentoring for Scientists: I Have a Mentor, What Now?

    Type
    Blog Post
    Published
    March 6, 2014, 7:12 p.m.
    ...for making your meetings productive: Discuss logistics if appropriate:  How often will you meet? How ...smaller, actionable steps (e.g. Meet 10 PhDs with interesting careers, find a summer internship, etc.). It ...Check Out Angela Depace's Talk on "Precision and Plasticity in Mentoring"    ...

  6. Extracurricular Activities for a Strong Science Career Path

    Type
    Blog Post
    Published
    Jan. 21, 2014, 3:08 p.m.
    ... Which graduate student hasn’t been asked the question: “Academia or industry?” Once academia was the...opportunities beyond academia. Once a profession sounds interesting, TAKE ACTION! Taking action: Examples of extracurricular...'d like to learn more about next. Leave your suggestions in the comments section.  ...

  7. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

    Type
    Blog Post
    Published
    July 21, 2020, 1:15 p.m.
    ...ug/ml pure dsDNA. Use the following formula to estimate your DNA: Concentration (ug/ml) = A260 reading...sensitivity at low concentrations of DNA and it can’t distinguish between DNA and RNA. See our protocol about using...dyes that fluoresce when bound to DNA. The main distinction here is that these dyes, such as PicoGreen or...

  8. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    Published
    Sept. 6, 2017, 1:01 p.m.
    ...via restriction enzyme digestion, and used directly in DNA assembly will still yield scarless assemblies... well-designed fragment overlap sections, while still creating scarless plasmid inserts.  Want to Learn...

  9. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

    Type
    Blog Post
    Published
    July 27, 2017, 1:11 p.m.
    ...avenues previously unavailable to them. With these fantastic tools it’s now easy to monitor protein expression...loops on either side of an enzyme’s active site and still block enzyme function upon exposure to ultraviolet...sample. The second sensor is a fusion protein consisting of sfGFP fused to a His-tagged ABC transporter...

  10. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    Published
    March 1, 2018, 2:11 p.m.
    ... by an anterograde AAV was shown to drop off drastically with lower titers. As you can see in figure 2...many more viral particles to your solution while still using a volume that’s compatible with your experimental...fewer opportunities your viral particles have to stick to your tubes, the higher titer you will have. When...

  11. Cultivating Community Science at BosLab

    Type
    Blog Post
    Published
    Nov. 2, 2017, 1:35 p.m.
    ...companies and BosLab has grown into an organization consisting of over twenty members and volunteers. Transforming... prior to getting started. For example, Timothy Stiles joined BosLab last year with a computer science...developed community projects. Recently, Timothy Stiles and Mrina Nikrad have started the Open Yeast Engineering...

  12. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    Published
    April 9, 2020, 1:15 p.m.
    ...human proteins that can be targeted (hopefully by existing therapeutics that are safe, effective, and already...ways by the pandemic, it is clear that we have to stick together (6 feet apart), to fight the new Coronavirus... all people, not just scientists! *If you are requesting plasmids for coronavirus or COVID-19 research...

  13. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    Published
    Oct. 19, 2023, 1:15 p.m.
    ..., B-B’, and C-C’) that give the feature its characteristic T-shape. The arrangement of the B-B’ and C-...elements of AAV and rAAV vectors, modifications can drastically affect gene transfer efficiency and transgene... efficiency. Our practical knowledge of ITRs is still limited, but this area of research is important ...

  14. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    Published
    April 14, 2016, 2:30 p.m.
    ...enough to engineer it to suit our purposes? These questions can be addressed using R bodies: simple, force-generating... modified to extend at different pH levels. Domesticating R bodies We have shown that all three of these...extends to rip the bacteria apart; however, they still remain attached to the ciliate. It’s thought that...

  15. Management for Scientists: Delegating is Key

    Type
    Blog Post
    Published
    Feb. 20, 2015, 8:30 p.m.
    ...understands that no question is “dumb” and that it is better to ask before wasting time. Focus on results...support. The manager must be available to answer questions. If you are perceived as too busy to be approachable...

  16. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    Published
    March 25, 2014, 3:15 p.m.
    ... not naturally exist in mammals, scientists can still reap the benefits of plasmid-based research using...that you may not want the plasmid depleted, but still want to use transient transfection methods. Since...* Mode of Action** Working Concentation*** Blasticidin bsd  HeLa, NIH3T3, CHO, COS-1, 293HEK Inhibits...

  17. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ...Anterograde and retrograde tracing take advantage of existing transport pathways in neurons. Anterograde transport...neuroscientists for some time due to their advantageous characteristics for brain research and their ability to introduce...Addgene). The Gradinaru Lab at the California Institute of Technology generated novel AAVs (Chan et al...Samaranch, L., Kells, A.P., Mittermeyer, G., San Sebastian, W., Zhou, S., Beyer, J., Forsayeth, J., and Bankiewicz...

  18. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    Published
    June 29, 2017, 1:06 p.m.
    ...promoters, origins, tags, and certain ORFs. Their sophisticated detection algorithm easily and precisely identifies...Sequence” tabs. Primers This tab contains a table listing commonly used primers detected in a given nucleotide...thanks to you (our community of scientists) for suggesting these changes, and the SnapGene and Addgene software...

  19. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type
    Blog Post
    Published
    June 1, 2017, 2:30 p.m.
    ...an important feature of a fluorophore. The key question is how the brightness is best defined or determined... cells within the dish. This is caused by the stochastic nature of plasmid uptake by cells. To correct...precise determination of brightness in cells, under realistic experimental conditions. By determining the brightness...

  20. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    Published
    May 28, 2020, 1:15 p.m.
    ...control AAV in smaller aliquots of 20ul for serotype testing to help you pick the best AAV for your experiment...GABA-ergic interneurons (83895). See our Serotype Testing AAV inventory for the entire collection! Plasmid...next 6 months, pending quality control. For an estimate on availability date or to be notified when a ...
Showing: 481 - 500 of 847 results