We narrowed to 497 results for: SUB

1. CRISPR 101: Homology Directed Repair

   Type

   Blog Post

   Published

   Jan. 26, 2023, 2:15 p.m.

   ... ssDNA 3’ overhang. This will serve as both a substrate for proteins required for strand invasion and ...

2. Multiple Plasmids at a Low Price: Kits from Addgene

   Type

   Blog Post

   Published

   Sept. 14, 2016, 2:30 p.m.

   ...used to purify proteins from E. coli, Bacillus subtilis, or Pichia pastoris depending upon your specific...

3. Evolution of Lab Techniques

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   Blog Post

   Published

   June 21, 2016, 2:30 p.m.

   ...screen multiple colonies before selecting a smaller subset to sequence. Sequencing gels and next-generation...

4. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

   Type

   Blog Post

   Published

   May 10, 2016, 2:30 p.m.

   ...course of infection, when HIV-1 is limited to a subset of T-cells, but it is unclear if enough T-cells...

5. Thoughts on the Future of Research 2015 Boston Symposium

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   Blog Post

   Published

   Nov. 25, 2015, 4 p.m.

   ...had been more cognizant of this during my PhD. Submitting a publication doesn’t have to be like throwing...

6. Hot Plasmids: Spring 2025

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   Blog Post

   Published

   June 3, 2025, 1:15 p.m.

   ... to target and manipulate distinct interneuron subtypes. Neuron, 113(10), 1525–1547.e15. doi: https://...

7. Viral Vectors 101: AAV Serotypes and Tissue Tropism

   Type

   Blog Post

   Published

   Jan. 16, 2025, 2:15 p.m.

   ...designs and administration doses. Don't forget to submit your validation data after your experiment! Thanks...

8. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

   Type

   Blog Post

   Published

   April 5, 2018, 12:42 p.m.

   ...efficiently integrate into the DNA through an HDR subpathway. It's traditionally been difficult to produce...

9. Antibodies 101: Multiplex Immunofluorescence

   Type

   Blog Post

   Published

   May 23, 2023, 1:15 p.m.

   ... J., & Trimmer, J. S. (2019). A toolbox of IgG subclass-switched recombinant monoclonal antibodies for...

10. PiggyBac-ing Through the Genome Editing Field

    Type

    Blog Post

    Published

    May 31, 2016, 3:30 p.m.

    ...marker such as thymidine kinase, a researcher may subsequently kill off any cells that still contain the donor...

11. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type

    Blog Post

    Published

    Dec. 17, 2020, 1:30 p.m.

    ...non-homologous sequence or a series of single-nucleotide substitutions along a 1.5 kb tract, all without selectable...

12. Antibodies 101: Validation

    Type

    Blog Post

    Published

    March 24, 2022, 1:15 p.m.

    ...antibody that you want to use to look at the subcellular localization of a protein of interest. Conveniently...

13. Antibodies 101: Designing Your First Flow Panel

    Type

    Blog Post

    Published

    May 28, 2024, 1:45 p.m.

    ...brightness levels, these differences are much more subtle (think of brightness levels on your smartphone ...

14. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type

    Blog Post

    Published

    April 25, 2017, 2:30 p.m.

    ...297.5588 (2002): 1873-1877. PubMed PMID:12228718. 2.Subach, Fedor V., et al. "Photoactivatable mCherry for...

15. A Needle in a Base-Stack: Cas9 Structural Biology

    Type

    Blog Post

    Published

    June 4, 2024, 1:15 p.m.

    ...Cas9’s two major lobes, REC and NUC, and their subdomains. NUC includes the HNH and RuvC catalytic domains...

16. Antibody Validation for Flow Cytometry

    Type

    Blog Post

    Published

    Sept. 10, 2024, 1:15 p.m.

    ...markers characterized so far, and the additional subtypes, are available here, and include information on...

17. Deep Dive: qPCR

    Type

    Blog Post

    Published

    Sept. 29, 2022, 1:15 p.m.

    ...lines and/or tissue types.  Please do note my oh-so-subtle use of reference genes, plural. Your data will ...