We narrowed to 495 results for: SUB;
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TypeBlog PostPublishedJune 21, 2016, 2:30 p.m....screen multiple colonies before selecting a smaller subset to sequence. Sequencing gels and next-generation...
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Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?
TypeBlog PostPublishedMay 10, 2016, 2:30 p.m....course of infection, when HIV-1 is limited to a subset of T-cells, but it is unclear if enough T-cells... -
Thoughts on the Future of Research 2015 Boston Symposium
TypeBlog PostPublishedNov. 25, 2015, 4 p.m....had been more cognizant of this during my PhD. Submitting a publication doesn’t have to be like throwing... -
Antibodies 101: Multiplex Immunofluorescence
TypeBlog PostPublishedMay 23, 2023, 1:15 p.m.... J., & Trimmer, J. S. (2019). A toolbox of IgG subclass-switched recombinant monoclonal antibodies for... -
Easi-CRISPR: Generating Knock-In and Conditional Mouse Models
TypeBlog PostPublishedApril 5, 2018, 12:42 p.m....efficiently integrate into the DNA through an HDR subpathway. It's traditionally been difficult to produce... -
Hot Plasmids: Spring 2025
TypeBlog PostPublishedJune 3, 2025, 1:15 p.m.... to target and manipulate distinct interneuron subtypes. Neuron, 113(10), 1525–1547.e15. doi: https://... -
Viral Vectors 101: AAV Serotypes and Tissue Tropism
TypeBlog PostPublishedJan. 16, 2025, 2:15 p.m....designs and administration doses. Don't forget to submit your validation data after your experiment! Thanks... -
PiggyBac-ing Through the Genome Editing Field
TypeBlog PostPublishedMay 31, 2016, 3:30 p.m....marker such as thymidine kinase, a researcher may subsequently kill off any cells that still contain the donor... -
An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency
TypeBlog PostPublishedDec. 17, 2020, 1:30 p.m....non-homologous sequence or a series of single-nucleotide substitutions along a 1.5 kb tract, all without selectable... -
Antibodies 101: Validation
TypeBlog PostPublishedMarch 24, 2022, 1:15 p.m....antibody that you want to use to look at the subcellular localization of a protein of interest. Conveniently... -
Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins
TypeBlog PostPublishedApril 25, 2017, 2:30 p.m....297.5588 (2002): 1873-1877. PubMed PMID:12228718. 2.Subach, Fedor V., et al. "Photoactivatable mCherry for... -
Antibodies 101: Designing Your First Flow Panel
TypeBlog PostPublishedMay 28, 2024, 1:45 p.m....brightness levels, these differences are much more subtle (think of brightness levels on your smartphone ... -
A Needle in a Base-Stack: Cas9 Structural Biology
TypeBlog PostPublishedJune 4, 2024, 1:15 p.m....Cas9’s two major lobes, REC and NUC, and their subdomains. NUC includes the HNH and RuvC catalytic domains... -
Antibody Validation for Flow Cytometry
TypeBlog PostPublishedSept. 10, 2024, 1:15 p.m....markers characterized so far, and the additional subtypes, are available here, and include information on... -
Deep Dive: qPCR
TypeBlog PostPublishedSept. 29, 2022, 1:15 p.m....lines and/or tissue types. Please do note my oh-so-subtle use of reference genes, plural. Your data will ...