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...might help to get you started. Once you have established a well working protocol in your lab, it can become...

...our CRISPR blog posts Download our CRISPR cheat sheet Download our CRISPR eBook Resources on Addgene....

...exist to solve the problem. What will you have accomplished in 5-10 years? You need to be able to describe...

...our educational resources. From 2013–2023, we published 110 videos, which received over two million views...

...work — in their system. The data is curated and published with a DOI, allowing others to reference and cite...

..., Rhodopseudomonas spp., Rhodospirillum spp., Shewanella spp., Thiobacillus spp., Xanthomonas campestris...

...Originally published Nov 12, 2015 and last updated Aug 20, 2020. Cas9 can be used to modify any desired...

...have been used successfully in experiments and published in peer reviewed journals. If you need to generate...

...express Anabaena GVs in mammalian cells without the shell stiffening protein GvpC, which allowed the GVs to...

... Alyssa Shepard...

...sequences are translated into amino acids. And I was astonished that the molecular language shared by every living...

...References and Resources Glossary Here’s a quick refresher if you ever lose track of which acronym is which...

... clinical grade hiPSCs is sold at $11,000 at ThermoFisher Scientific and can be used for only 6-8 reprogramming...

...Cassel SH, Vazquez F, Weir BA, Hahn WC, Marsh JA, Tsherniak A, Kadoch C (2018) Interrogation of Mammalian ...

...possible transduction. Be consistent Once your lab establishes the best conditions for specific applications...

...better at 30°C or room-temperature, which is accomplished by growing your culture to the desired density...

...1731-1745. PubMed PMID: 26960991. 9. Chatterjee, Abhishek, et al. "A genetically encoded fluorescent probe...

...molecules per cell volume? A call to rethink some published values." Bioessays 35.12 (2013): 1050-1055. PubMed...

... which results in a merodiploid. This can be accomplished by selecting for an antibiotic resistance marker...

...dermis and identify that Langerhans cells are replenished through the proliferation of specific, and not...