...grows resulting in the appearance of unmethylated sites in the chromosome. The more stable endonuclease ...an immune response. As a proof of concept, they tested a lux-mediated bioluminescence reporter in vivo...hok/sok system of plasmid R1: Sok antisense RNA regulates hok gene expression indirectly through the overlapping...

...demonstrated that targeting dCas9 to transcriptional start sites was sufficient to repress transcription by blocking...of activators include the SunTag system which facilitates recruitment of multiple activation domains to...2020). Moreno-Mateos, M. A. et al. CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled...

...role of the MECP2 gene, and highlights some of the latest breakthrough therapeutic approaches. What is Rett... the work of the consortium, two gene therapy candidates advanced into clinical development programs in... syndrome gene therapy improves survival and ameliorates behavioral phenotypes in MeCP2 null (S51.002)...

...advantage of this system is that streptavidin conjugates are commercially available for a wide range of... heterogeneous cell mixes, like bulk lympho-/splenocytes, you might only be interested in one of the various...mummification). Permeabilization, as the name suggests, perforates the cell membrane to allow for antibodies to ...

...is its speed, labeling neighboring proteins in minutes rather than hours like BioID. APEX has allowed ...and miniTurbo both enable biotin labeling in 10 minutes without any issues in toxicity. TurboID’s signal...give these methods a try.  References:  Branon, Tess C., et al. "Efficient proximity labeling in living...

...saying something like, “I’ll be there in a few minutes, I just need to split some cells.” Inevitably, ... Word or google doc that I can comment and make notes on as I progress. I’ve also recently started using...Business Review article on procrastination recommends testing out how much time you can possibly handle doing...

...GFP-PASS (green). After 30 minutes of blue light illumination, substantial PA accumulates in the plasma membranes...optogenetics plasmids – and keep an eye out for future updates to the collection! Resources and references Additional...

...propagated and have a generation time of around 20 minutes, they are perfect for molecular cloning and metabolic...is far from perfect. One of the most limiting attributes of rats is their difficult to manipulate genome...making them useful for in vivo studies. These attributes make frog eggs some of the most useful tools ...

...speakers talk without looking at their slides or notes. Listen to their stories and narratives (see below... that you can deliver most of your talk without notes. Don’t be hard on yourself if you stumble or make...more engaging session. The best speakers use no notes and can concentrate on delivery, rapport with the...

...them in 1885. E. coli are mainly found in the intestinal tract of animals. There are many different naturally...coli used today are descended from two individual isolates, the K-12 strain and the B strain. K-12 was isolated...typically standard; just having the gene listed indicates it is non-functional. If a gene is deleted, it...

...expected fragments for that plasmid. This phenomenon indicates the presence of a multimer.   Fig. 1:... be used to detect multimers, since restriction sites in multimers usually repeat in tandem along with... of the plasmid monomer (see this article from BiteSize Bio for more information on how different plasmid...

...for the PI and lab members.  Make coffee or beer dates to talk to as many lab alumni as you can or schedule...or at least observe grant writing? Get equipment quotes? Understand administrative responsibilities? Is...this blog as a guide. Start a document to record notes and observations so you have data to review when...

...aware of any possible alternative translation start sites or alternative splicing that could lead to some ...with your protein of interest and show just the faintest band from the cells transfected with the other...work great in another context! So the next time you test an antibody (perhaps one from Addgene!), consider...

...Each path to science policy is unique and this creates a rich and diverse workforce. Out of all the career...interests.  Action Items:  Sign up to receive updates from ESAL and explore their Local Engagement Playbook...others (see more in Figure 2). Starting early demonstrates not only your interest to future positions, ...

...improvement over Cas9-mediated HDR for the loci tested, where average HDR-mediated editing frequency was...sequences, expanding the number of available target sites for base editing (Kim et al., 2017). For each base...have an improved base editing capacity, even at sites previously difficult to target. ABE8s can achieve...

...holiday spirit by kicking off our annual #DeckTheLab contest. The bar is set very high from last year’s impressive...some cheer by snapping a photo of you and your labmates celebrating the holidays and tweeting it with ...

... first edition of our Antibodies 101 eBook! The latest addition to our eBook collection is a handy resource...start looking for answers.    We hope you find the latest addition to our eBook collection useful. And, as...

...celebrate the 12 Days of CRISPR with some of the latest and greatest in CRISPR tools and resources! Ready to sing...

...Update Apr 8, 2021: While this contest is over, we will host other contests in the future. Subscribe to ...

... spent the last week perusing the Deck The Lab contest submissions on Twitter and Instagram and we’re ...these creative entries as the winners of the 2019 contest. Agar art For the second year in a row, agar ...