We narrowed to 877 results for: TOR

... take into account chromatin context and other factors, they are easy to use and can reduce off-targets...nicking/cleaving of DNA occurs, not for deadCas activator/repressor experiments where only localization ...- GUIDE-Seq and integrase-defective lentiviral vectors - which rely on insertion of DNA by the NHEJ pathway...RNA-seq would be an appropriate method for an activator or repressor Cas. Similarly, Cas ChIP-Seq would... (e.g. in a gene in a similar pathway, a growth factor, etc.) this control is usually not very meaningful...and off-target risks will vary depending on many factors. Hopefully now you have all the resources you need...

...and luckily genetics is a sector which has been rapidly growing. These factors left me optimistic that ...and the pro’s and con’s of working in different sectors. Luckily, I have worked for a variety of different...options for going into parasitology but another major factor held me back; I do not have a second language. ...allowed me to try out working in many different sectors and to discover for myself what potential job opportunities... Are Open at Addgene Learn How to Choose a Good Mentor for Your Professional Development ...

...Chinmaya Sadangi, a postdoc at the University of Toronto. The Addictive Brain was founded in early 2018 ...Chinmaya Sadangi, a postdoc at the University of Toronto, created The Addictive Brain to spread awareness...non-scientists can directly approach scientists and doctors with their questions and comments.  Lastly, we ...Keep your message to the main three points of your story. Interested in learning more? Visit their website...Sadangi is currently a postdoc at the University of Toronto and founder of The Addictive Brain. Prior to this...

...signaling activity and cell phenotype. One method for monitoring live single-cell kinase activity is FRET, but... distance and the orientation of the donor and acceptor proteins, which can be difficult to predict. ...disadvantages when compared to FRET reporters. First, KTRs monitor global kinase activity that occurs in the cytoplasm...experiments. Third, KTRs are not well suited for monitoring kinase activity that occurs during nuclear envelope...the nucleus, there’s no nuclear localization to monitor). Whether you’re looking for a ready-to-use KTRs...

...here!   Marty LaFleur is a Postdoctoral Fellow in Arlene Sharpe’s laboratory at Harvard Medical School ...Cas9) hematopoietic stem cells with a lentiviral vector containing a gRNA. These transduced stem cells ...custom mouse strains and lentiviral expression vectors (Table 1). The four systems Name Function...G., & Sharpe, A. H. (2024). X-CHIME enables combinatorial, inducible, lineage-specific and sequential ...

...The first is the study of non-coding regulatory elements. Regulatory DNA function is typically inferred ...interested in exploring the function of non-coding regulatory elements in their native chromatin context. An...Bauer is a pediatric hematologist and principal investigator at Boston Children’s Hospital and Harvard Medical...PhD student at Harvard Medical School in the laboratories of Stuart Orkin and Daniel Bauer. He received...

... successful, internationally renowned plasmid repository that it is today. Although I wish I could take... attributed to the entire Addgene family; the depositors and requesting scientists, all of the Addgene..., Addgene supporters, and of course, Executive Director, Joanne Kamens, and founders, Melina Fan, Ken ...about her grandfather’s humble beginnings as an operator of a printing press. Just as her grandfather used...Joanne shared a moving thank you email from a requestor in Iraq (Addgene's first). After the speeches,...

...wanted users to be able to find plasmids and viral vector tools more easily. In the redesign, the new search...unique: community, sharing, and fun. The Addgene repository is built on the foundation of a community that...dictate the space. We wanted to comfortably tell our story, to get scientists excited about what Addgene has...redesigning the homepage, and striving to tell our story in better ways, we realized that Blugene needed ...you come to Addgene to find the plasmid or viral vector for your next experiment, that you can find what...

...  Dr. Sibby Anderson-Thompkins, Director of the Office of Postdoctoral Affairs at UNC, called her presentation...current postdocs will be academic principal investigators. She also pointed out promising new practices...increasing the value and priority put on good mentoring and applauded the new requirement for PIs to include...opened with a discussion of the Novartis industry postdoctoral program.  His strongest comments were about ...

... markers all tagged with mEmerald. Plasmids to monitor protein-protein interactions through multicolor... Baird said. “They are all designed in the same vector backbone with very similar restriction enzymes....Michael Davidson told the Tallahassee Democrat in a story about his life’s work and the stage IV lung cancer...shutter its doors. “We wanted to give all of these vectors to the scientific community and contribute in a...community," says Joanne Kamens, Addgene's Executive Director, "and to promote the great work of Michael Davidson...

...chance vs. the factor that is being experimentally manipulated. Below are several core factors of statistics...variables – factors which are not affected by other variables (cause). Dependent variables – factors which...total crop production and the production window. Factorial ANOVA This type of ANOVA assesses multiple independent...sounds like a two-way ANOVA. And this time, it is! Factorial ANOVAs include two-way ANOVAs but can include ...

...technologies, one might incorrectly relegate RNAi to history. The straightforwardness of RNAi is unmatched—unlike...cannot be used to investigate essential genes. Historically, gene silencing methods were thought to be afflicted...considerations, such as the starting material (DNA-based vector or purified RNA) and the initial state of the dsRNA... Although downstream work is required, several vectors can be found at Addgene that could serve as an ...an excellent start (#22988, #137884). Both vectors allow the creation of amiRNAs based upon miRNA precursors...method. Instead of using a fluorescent output to monitor RNAi, we engineered a positive selection reporter...

...making Laos the 112th country that we support. This story is a vivid example of how the global scientific ...advanced. Behind every publication is another great story, like researchers in India using Addgene-shared ...asked for a smaller size for our on-demand viral vector packaging service to better match experiment needs...Whether you're depositing plasmids, ordering viral vectors, or exploring our educational resources, you're...research. Thank you for letting us be part of your story, and for being such an important part of ours. We...

...model for cancer-specific research. Membrane receptors and ion channels are often exogenously expressed... degrade over time in culture. This can affect factors that could skew research results (gene expression... Especially if your research interests include receptor biology, ion channels, or involve human protein...Platform to Produce Recombinant Proteins and Viral Vectors. Front Bioeng Biotechnol. (2021) (9):796991. DOI...10 Tips for Mammalian Cell Culture Adenoviral Vector Production and Troubleshooting Additional resources...

...Willoughby.   Using RUBY as a selection marker The creators of RUBY highlight its use in crop plants under... is difficult for new members of the lab or collaborators unfamiliar with these mutant plants. Using RUBY...promoter with self-cleaving 2A site linkages and a terminator. Image from He, Y., Zhang, T., Sun, H. et al...., Sun, H. et al. A reporter for noninvasively monitoring gene expression and plant transformation. Hortic...Transformation CRISPR 101: CRISPR-Mediated Plant Base Editors ...

...memorable logo DNA was an obvious place to start brainstorming our new logo. It literally underpins our DNA-derived...sketched hundreds of ideas: Figure 3: Logo brainstorming. The winner shows a twist of DNA forming...so much more Addgene started out as a plasmid repository. Over the years, we recognized that what we offer...Addgene also offers other materials like viral vectors and antibodies. Plus, we’ve invested in data sharing...Addgene’s Blue Flame Award is prized among our depositors. Marking plasmids that have been requested over...

...of autophagic substrates inside the lysosome. Historically, methods for studying autophagy focused on counting... autophagic activity, and are a more reliable indicator of autophagic activity. The aim of this post is...mitochondria-targeting sequence makes Keima useful for monitoring mitophagy. It’s important to remember that because...Maria Cuervo, and Per O. Seglen. "Methods for monitoring autophagy from yeast to human." Autophagy 3.3...PMC4517063. Yoshii, Saori R., and Noboru Mizushima. "Monitoring and measuring autophagy." International journal...

...different story altogether? The unfortunate answer is yes, largely due to a few limiting factors.  To start...spent your life making connections and finding mentors -- well, it's time you call upon these people for... is more important than the product itself -- investors would rather fund excellent people with mediocre...Harvard/MIT faculty, venture capital and angel investors, and business development heads at major companies...

...pitfalls: they require construction of plasmid vectors and/or rely on cumbersome in vitro synthesis of... have low knock-in efficiency. Together, these factors prevent stem cell researchers from generating clonal...purchased and there is no need to construct plasmid vectors or synthesize/purify IVT sgRNA in the lab. Figure...experiments. Notably, we could tag >60 transcription factors in a short (3-week) time window; this was possible...csRNP experiments, it can be ordered in bulk and stored in small aliquots at -80 oC. Assemble csRNP: Anneal...success of knock-in experiments depends on many factors. Some of these include: Most importantly, the...

...researchers, it can expose them to a new institute and mentor that they may want to consider for future work ...professor or institute, ask if you will have a bench mentor or anyone else available to directly help you during... during your visit. Reach out to your bench mentor (if you have one) to communicate your plan as well.... comfortable initiating this conversation, your mentor can help get the ball rolling! If you are applying...techniques. Show up on time (don’t make your bench mentor wait on you!), make sure you are prepared (read...