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We narrowed to 743 results for: TRI

Showing: 481 - 500 of 743 results

  2. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    Published
    Sept. 7, 2017, 12:17 p.m.
    ...This post was contributed by guest blogger Iris Lindberg, Professor at the University of Maryland School...Cloning cells using an agarose overlay does have one tricky step. It is necessary to pour the agarose onto ...

  3. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    Published
    Feb. 4, 2016, 3:30 p.m.
    ...under which the plasmid is distributed.  All Addgene plasmids are distributed under the terms of the UBMTA...

  4. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    Published
    Aug. 18, 2015, 2:30 p.m.
    ...are treated with a drug of interest and gRNA distribution is analyzed in the drug-resistant population...6 gRNAs per target gene, and maintaining the distribution of each gRNA within the population is key. Loss...

  5. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...This post was contributed by Jae Lee and Pantelis Tsoulfas of the Department of Neurological Surgery ...along the illumination path. The shadows appear as stripes in the acquired image (5).  A way to get around...

  6. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...multicistronic vectors work? Scientists have "borrowed" some tricks discovered in positive single-stranded RNA viruses...use the 2A peptides instead of an IRES when stoichiometrically equivalent levels of expression are required...

  7. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...This post was contributed by Gal Haimovich of greenfluorescentblog. Be honest. Do you really know how... short as 100ms (EBFP) or as long as 1 hour (mAmetrine1.2). However, for most FPs it is a few seconds ...

  8. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    ...noticing unusual cases of acute hepatitis in the pediatric population. Once ~400 cases had amassed in places...removal of certain adenoviral genes that apparently contribute to virulence (Tollefson et al., 1996) – cell ...

  9. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    Published
    Dec. 22, 2022, 2:15 p.m.
    ...’ve got you covered in this blog, with tips and tricks for harnessing the homology-directed repair pathway...are overly confluent or don’t have sufficient nutrients in their media, they will not undergo DNA replication...

  10. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    Published
    Sept. 6, 2022, 1:15 p.m.
    ...conditions commonly encountered in human gene therapy trials. Human Gene Therapy Methods, 26(2), 71–76. https...Qu, G. (2005). Identification of factors that contribute to recombinant AAV2 particle aggregation and ...

  11. Tips for arabidopsis transformation

    Type
    Blog Post
    Published
    Oct. 25, 2018, 1:23 p.m.
    ...This post was contributed by Laura Lee, a graduate student at Stanford University. Arabidopsis is a fantastic... it pretty much is, but there are some tips and tricks you can use to obtain optimum transformation efficiency...

  13. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Oct. 14, 2015, 2:30 p.m.
    ...functions differently. Since Cpf1 loci are widely distributed across bacterial species, Zetsche et al. hypothesized...directional gene transfer, analogous to traditional restriction enzyme cloning. Sticky-end mediated gene transfer...

  14. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    Published
    Aug. 20, 2015, 2:30 p.m.
    ... This post was contributed by Jo Miller, founder and principal of J. Miller & Associates, Inc., a national...professionals is learning to succeed in grants through trial and error, through winning and losing funding, and...

  15. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ...them! Note: A. Max Juchheim, Marcy Patrick, and Meghan Rego contributed to the writing of this article. ...

  16. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    Published
    March 20, 2014, 3:04 p.m.
    ...an elderly family member, want to train for a triathlon, finally buy that charming fixer-upper house you... every school performance, chaperone every field trip or cheer at every soccer game. Tip: take turns going...

  17. How to Be an Excellent Trainee

    Type
    Blog Post
    Published
    Sept. 26, 2023, 1:15 p.m.
    ...than any other one. Luckily, you can do a lot to contribute to the relationship dynamic and set yourself ...notes on general protocol steps and other tips and tricks your mentor shares. Additionally, go ahead and ...

  18. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    Published
    July 13, 2023, 1:15 p.m.
    ... libraries are typically DNA preps, but we do distribute several of our most popular CRISPR pooled libraries...cells at a concentrated seeding density while centrifuging. Spinfection transduction efficiency can vary...

  19. Transferable Skills Guide: Creativity

    Type
    Blog Post
    Published
    Jan. 18, 2022, 2:15 p.m.
    ...kill your productivity. Harvard Business Review. Retrieved January 14, 2022, from https://hbr.org/2019/11...innovation on your team. Harvard Business Review. Retrieved January 14, 2022, from https://hbr.org/2019/07...

  20. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    Published
    May 20, 2025, 1:15 p.m.
    ...the host organism being unable to synthesize a nutrient required for growth. Before delivery of the vector...cells are grown in media that supplies the key nutrient. The vector provides the missing synthesis machinery...
Showing: 481 - 500 of 743 results