...Clearly we have all of our writers to thank for the excellent content they’ve churned out in the last month....or G->A transitions in a target sequence in live cells. This technique uses catalytically inactive CRISPR...and other means of generating mutations in live cells are all discussed further in this informative post...

...Biette KM, DePace AH. Mol Cell. 2015 Jun 4;58(5):718-21. doi: 10.1016/j.molcel.2015.04.025. PubMed PMID..., set tangible research and career goals, and celebrate accomplishments. These techniques are laid out...

...20th anniversary. Throughout the year, we’ll be celebrating with stories of innovation and impact made possible...enjoy these stories, and other 20th anniversary celebrations, by checking out our blog, social media, newsletter...nonprofit status to achieve mission-driven goals of accelerating research and discovery by improving access to...

...opportunities, protocols, publications, and more to accelerate the pace of research into COVID-19. While this...opportunities, and community response initiatives. Accelerate the translational impact of research Universities...important for creating research synergies and accelerating the translational impact of research activities...

...give you a chance at an interview:  1) Have an excellent resume ready to be submitted The structure and...successful application. It takes time to prepare an excellent resume…it is worth it. Read here to get help if...if you are having trouble preparing your most excellent resume. 2) Follow job boards and check them regularly...

...antigens in living cells or tissues. sdAbs can also be used to manipulate cellular functions, like in ...sdAbs over conventional antibodies include:  Excellent tissue penetration, thanks to their small size...

...fluorescent protein (GFP) was the reporter gene and green cells were observed in the kidneys at a specific developmental...genes of interest may not be expressed in the tissue/cell line you are working with. Development-specific...10.7554/eLife.67667 Springer, S. P. Gene Traps. Plant Cell. (2000) 12(7): 1007-1020. DOI: 10.1105/tpc.12.7.1007...

...fulfilling our mission. “Addgene’s nonprofit mission: Accelerate research and discovery by improving access to...profit motive. We build this trust by keeping excellent records and maintaining confidentiality where ...nonprofit, open science and Addgene in our mission to accelerate research with easier sharing and collaboration...

...the brain has been in vivo microdialysis of extracellular fluid (Darvesh et al., 2011). This technique... across the semipermeable membrane from the extracellular fluid into the perfused liquid. The probe then...detection of dopamine in flies, fish, and mice." Cell 174.2 (2018): 481-496.PubMed PMID: 30007419. PubMed...

...Fluorescence Activated Cell Sorting (FACS), allowing for enrichment of successfully targeted cells that can be used...investigating genetic mechanisms related to morphogenesis, cell fate specification, and human disease.     Sun,...

...team in Paris, France. While efforts like these accelerate the progress of research, they can also present...helps everyone feel included in the project and accelerates the pace at which problems can be addressed and...the times that work best for them. This also accelerates the pace of research by circumventing the slower...

...predict that we’d be able to edit RNA in mammalian cells with CRISPR? I certainly didn’t, but now we can ...updates We hope you enjoyed the #12DaysofCRISPR, a celebration of our updated blog resources. Not on social ...Improve HDR Efficiency for CRISPR Editing in Human Cells Day 3: Cpf1: Cpf1: A New Tool for CRISPR Genome ...

...properly. Directly transform chemically competent cells with the digested mixture. There is no need for ...purification before transformation. After transforming cells, you should perform colony PCR with vector specific...interested in protein science.     References: 1. Celie, Patrick HN, Annabel HA Parret, and Anastassis Perrakis...

...school at MIT. “I read some of the seminal papers on cell cycle discovery from Lee Hartwell and Paul Nurse...genetic screen to identify the factors that regulate cell division. And that really got me hooked on genetics...high-resolution tracking of more than 1 million cancer cells under drug treatment.” Stegmeier especially finds...

...graduate student studying plant development and cell-cell communication in the Nimchuk Lab in the Biology...untransformed seedlings or callus (undifferentiated plant cell culture), which is great for a wide variety of applications...

...would continue on with my academic dream of studying cell division. However, after 6 years in the business...Cambridge University where he studied mechanisms of cell division. Recently Seán got the entrepreneurial ...information on ELISA troubleshooting, ELISA kits and cell death assays. You can find Seán on twitter @Macfhearraigh...

...and see how different parts of the brain or the cells within them work together or perhaps compete against...two-photon properties, so you could activate single cells in arbitrary patterns in the brain. Addgene: Do ... different – an awake monkey is different from a cell in a dish. I always tell people, 'Be driven by your...

... Typically, Addgene uses electrocompetent Stbl4 cells when performing amplifications, and we use electroporation...DNA. This bias can be counteracted by growing the cells on solid plates, where each transformant forms its...own colony, uninfluenced by the growth of other cells. For libraries where the plasmids are all very similar...

...Voigt CA, Ellington AD. Cell. 2009 Jun 26;137(7):1272-81. doi: 10.1016/j.cell.2009.04.048. PubMed. Find...with sophisticated gene expression controls. Most cell types do not respond to low-intensity light, so ...

...to supplement your cells with biliverdin for maximal brightness. In mammalian cells, one of the improved...Imaging Center at UCSF. A common requirement for live cell imaging experiments is the ability to follow multiple...