Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 718 results for: cel

Showing: 481 - 500 of 718 results

  2. Addgene’s Top 10 Blog Posts from 2014

    Type
    Blog Post
    Published
    Dec. 16, 2014, 8:01 p.m.
    ...more about. Our post on “Making Your Own Competent Cells” has been extremely popular, generating many additional... We’re happy to see scientists making their own cells to save money and create the tools that work best...Save Time and Money by Making Your Own Competent Cells By Melina Fan Plasmids 101: The Promoter Region...

  3. Introducing an All-in-One CRISPR/Cas9 Vector System for Multiplex Genome Engineering

    Type
    Blog Post
    Published
    Nov. 12, 2014, 3:54 p.m.
    ...Multiplex genome engineering demonstrated in human cells They used the Golden Gate cloning method to ligate...successfully to target multiple sites in cultured human cells. While it has not yet been demonstrated, they say...Reference: Multiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector system. Sakuma...

  4. Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

    Type
    Blog Post
    Published
    Dec. 20, 2017, 2:33 p.m.
    ...This post was contributed by guest bloggers Lauren Celano of Propel Careers and Rachel Casseus, Esq. Founder...below. An international or national award for excellence in your field. Membership in a professional organization...counsel. Many thanks to our guest bloggers Lauren Celano of Propel Careers and Rachel Casseus, Esq. Founder...resident green cards and citizenship.   Lauren Celano, CEO Propel Careers. Lauren is passionate about...

  5. What's New in CRISPR - May 2019

    Type
    Blog Post
    Published
    May 28, 2019, 12:49 p.m.
    ...genome-wide deamination of RNA cytosines in human cells. Thus, they engineered two CBE variants with mutations...RNA edits by >390 fold and >3,800 fold in human cells with no significant reduction in editing efficiency...mice and in zygotes. Read the paper in Molecular Cell Find the Nme2Cas9 All-in-One AAV constructs CRISPR...

  6. How Exercise Purges Stress and Keeps Depression at Bay

    Type
    Blog Post
    Published
    Nov. 25, 2014, 4:02 p.m.
    ... how it works. The findings in a recent issue of Cell demonstrate how PGC-1a1, a transcriptional coactivator... in a commentary about the new work published in Cell Metabolism. But, they note, there is plenty more...JR, Erhardt S, Lindskog M, Ruas JL. doi: 10.1016/j.cell.2014.07.051. PMID: 25259918 Muscle over mind. Moon...

  7. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    Published
    March 11, 2014, 5:55 p.m.
    ...RNA-Mediated Gene Targeting in One-Cell Embryos. Niu Y, et al. Cell. 2014 January 30. (Pubmed) Programmable...correct disease mutations, both in mice and in human cells. But that important advance was quickly followed...

  8. Developing Transferable Skills During Science Training

    Type
    Blog Post
    Published
    June 3, 2014, 2:14 p.m.
    ...transferable skills while you are also learning to be an excellent scientist. Leadership and management Take a lead...supportive editors so your first drafts are always excellent Technical knowledge and creativity Become a “...local networking and trade organizations Another excellent perspective on this topic can be found on the ...

  10. Editor's Choice, August 2016

    Type
    Blog Post
    Published
    Sept. 2, 2016, 1:32 p.m.
    ...Clearly we have all of our writers to thank for the excellent content they’ve churned out in the last month....or G->A transitions in a target sequence in live cells. This technique uses catalytically inactive CRISPR...and other means of generating mutations in live cells are all discussed further in this informative post...

  12. Scientific Sharing in the Time of COVID-19: Databases and Resources

    Type
    Blog Post
    Published
    May 12, 2020, 1:15 p.m.
    ...opportunities, protocols, publications, and more to accelerate the pace of research into COVID-19. While this...opportunities, and community response initiatives. Accelerate the translational impact of research Universities...important for creating research synergies and accelerating the translational impact of research activities...

  13. 2023 and 2024: Reflections and Looking Ahead

    Type
    Blog Post
    Published
    Jan. 23, 2024, 2:15 p.m.
    ...20th anniversary. Throughout the year, we’ll be celebrating with stories of innovation and impact made possible...enjoy these stories, and other 20th anniversary celebrations, by checking out our blog, social media, newsletter...nonprofit status to achieve mission-driven goals of accelerating research and discovery by improving access to...

  14. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    Published
    July 9, 2015, 2:30 p.m.
    ...give you a chance at an interview:  1) Have an excellent resume ready to be submitted The structure and...successful application. It takes time to prepare an excellent resume…it is worth it. Read here to get help if...if you are having trouble preparing your most excellent resume. 2) Follow job boards and check them regularly...

  15. Fostering communication in remote collaborations

    Type
    Blog Post
    Published
    April 21, 2020, 1:14 p.m.
    ...team in Paris, France. While efforts like these accelerate the progress of research, they can also present...helps everyone feel included in the project and accelerates the pace at which problems can be addressed and...the times that work best for them. This also accelerates the pace of research by circumventing the slower...

  16. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    Published
    July 27, 2021, 1:15 p.m.
    ...Fluorescence Activated Cell Sorting (FACS), allowing for enrichment of successfully targeted cells that can be used...investigating genetic mechanisms related to morphogenesis, cell fate specification, and human disease.     Sun,...

  17. Sensing Neuronal Dopamine

    Type
    Blog Post
    Published
    July 30, 2019, 12:59 p.m.
    ...the brain has been in vivo microdialysis of extracellular fluid (Darvesh et al., 2011). This technique... across the semipermeable membrane from the extracellular fluid into the perfused liquid. The probe then...detection of dopamine in flies, fish, and mice." Cell 174.2 (2018): 481-496.PubMed PMID: 30007419. PubMed...

  18. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    Published
    Dec. 12, 2017, 3:13 p.m.
    ...predict that we’d be able to edit RNA in mammalian cells with CRISPR? I certainly didn’t, but now we can ...updates We hope you enjoyed the #12DaysofCRISPR, a celebration of our updated blog resources. Not on social ...Improve HDR Efficiency for CRISPR Editing in Human Cells Day 3: Cpf1: Cpf1: A New Tool for CRISPR Genome ...

  19. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    Published
    June 5, 2017, 3:19 p.m.
    ...fulfilling our mission. “Addgene’s nonprofit mission: Accelerate research and discovery by improving access to...profit motive. We build this trust by keeping excellent records and maintaining confidentiality where ...nonprofit, open science and Addgene in our mission to accelerate research with easier sharing and collaboration...

  20. FastCloning: A Newer, Simpler Cloning Technique

    Type
    Blog Post
    Published
    Nov. 1, 2016, 2:30 p.m.
    ...properly. Directly transform chemically competent cells with the digested mixture. There is no need for ...purification before transformation. After transforming cells, you should perform colony PCR with vector specific...interested in protein science.     References: 1. Celie, Patrick HN, Annabel HA Parret, and Anastassis Perrakis...
Showing: 481 - 500 of 718 results