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We narrowed to 811 results for: cha

Showing: 481 - 500 of 811 results

  1. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    Published
    July 28, 2016, 2:30 p.m.
    ...register and catalyzes their recombination, thereby changing the state of the RSM. If an attB-attP pair is ...general would benefit from a more comprehensive characterization of the recombinases at our disposal.  Many...Vectors Can Be Used for Genome Editing Learn about Michael Koeris' Journey from Grad Student to SynBio Entrepeneur...

  2. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    Published
    Feb. 18, 2016, 3:42 p.m.
    ...matrix (usually agarose) to pull your negatively charged DNA through the gel. As indicated in the figure...the gel in wells positioned toward the cathode (- charge). When the voltage is applied across the gel, the... the DNA migrates toward the anode (+ charge). Larger fragments of linearized DNA migrate slower than ...

  3. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    Published
    Nov. 2, 2021, 1:15 p.m.
    ... Figure 2. Schematic diagram showing the mechanism of spontaneous reversion during AAV plasmid amplification...phenotype in a mixed population (Burger, C. 2004, Aschauer, DF. 2013, O’Carroll, SJ. 2021). This is due to...outcome.    References and resources References Aschauer DF, Kreuz S, Rumpel S (2013) Analysis of Transduction...

  4. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    Published
    March 7, 2023, 2:15 p.m.
    ...quantifies the likelihood that a result is due to chance vs. the factor that is being experimentally manipulated...) or skewed (see Fig. 2). Assumptions – characteristics of a data set; the ‘rules’ a statistical test...trial, for example).   Fig. 3: Question flowchart for choosing an ANOVA/MANOVA statistical test ...

  6. Antibody Data Repositories and Search Engines

    Type
    Blog Post
    Published
    Oct. 31, 2024, 1:15 p.m.
    ...Antibodypedia Any Any Data repository   YCharOS Human proteins Immunoblot (western blot), IP...BioMolecular Atlas Program (HuBMAP)  Antibody Characterization Laboratory Cancer Any Data repository...

  7. CRISPR Cheat Sheet

    Type
    Blog Post
    Published
    Jan. 27, 2021, 2 p.m.
    ...template, CRISPR can be used to introduce a functional change in the DNA sequence. Base Editors: Modified versions...versions of CRISPR nucleases that make single base changes in the DNA sequence without completely cutting ...

  8. New Podcast Segment: Hot Plasmids

    Type
    Blog Post
    Published
    May 17, 2017, 3:37 p.m.
    ...covered in this segment Hot Plasmid 1: CRISPR X from Michael Bassik's Lab - A new tool for making diverse, localized...this kit to complement unused auxotrophies in Saccharomyces strains.  Listen to Our Segment on The Yeast...

  9. Countdown to Halloween @Addgene

    Type
    Blog Post
    Published
    Oct. 29, 2014, 5:26 p.m.
    ...(old board games and movies can provide great character costumes). All of this costume planning is for...guess each plasmid? Our lab paid tribute to the changing seasons – and they also invited Father Time to...

  10. Congratulations, Deck The Lab winners!

    Type
    Blog Post
    Published
    Jan. 14, 2021, 1:30 p.m.
    ...created a microbe advent tree displaying over 25 archaea, bacteria, fungi, and viruses. Final tree with...MicrobeAdventCalendar ornament set (plus more!) It features archaea, bacteria, fungi, viruses, and one microbe ornament...

  11. Newly Updated AAV Data Hub!

    Type
    Blog Post
    Published
    Feb. 7, 2023, 2:15 p.m.
    ...dendritic spines!  Curious? Watch our AAV Data Hub Challenge recording to see two reports presented by the ...report describes a functional vector such as a channelrhodopsin Images showing the injection site as well ...

  13. Make a Splash: Notions of Scientific Impact Are Evolving

    Type
    Blog Post
    Published
    Dec. 5, 2014, 2:54 p.m.
    ... Considering citations to all articles, Anurag Acharya and colleagues report, the percentage of citations...has increasingly moved online, surely times have changed. Altmetrics.org calls to expand our view of what...

  14. A Scientist's Guide to Ebola

    Type
    Blog Post
    Published
    Oct. 16, 2014, 3:01 p.m.
    ...through the air and that’s considered unlikely to change – but the illness has been fatal in 70 percent ... Ebolavirus (EBOV) is a Filovirus, a family characterized by single, linear, negative-sense, single-stranded...

  16. Download Science-Themed Zoom Backgrounds from Addgene

    Type
    Blog Post
    Published
    April 22, 2021, 1:15 p.m.
    ... Zoom’s default backgrounds all year and want a change? Addgene has you covered. Today we’re releasing...next meeting.     If you’re reading this blog, chances are you are a fan of Addgene or our very cute mascots...

  17. How Exercise Purges Stress and Keeps Depression at Bay

    Type
    Blog Post
    Published
    Nov. 25, 2014, 4:02 p.m.
    ...depression, and now researchers have reported a mechanism based on studies of transgenic mice that helps...brain is protected from stress-induced biochemical changes that could otherwise lead to depression. And there...

  18. A Conference By Postdocs For Postdocs: Future of Research

    Type
    Blog Post
    Published
    Sept. 2, 2014, 3:05 p.m.
    ...must do science to be happy. What do we have to change to ensure that all scientists can have successful...training too many scientists and some propose radical changes to the way we view the graduate school and postdoc...

  19. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    Published
    March 11, 2014, 5:55 p.m.
    ...Scientists at North Carolina State, in the labs of Chase L. Beisel and Rodolphe Barrangou, had applied the...Powerful New Way to Edit DNA" and "This Protein Could Change Biotech Forever."  David S. Weiss of Emory University...

  20. Addgene's Top Blog Posts from 2020

    Type
    Blog Post
    Published
    Dec. 29, 2020, 2:15 p.m.
    ...many molecular biology methods such as allelic exchange, FRET, and synthetic biology. 7. 25 Work From...them in the cell. This blog post features two mechanisms that cause plasmids to be incompatible. 4. RNA...
Showing: 481 - 500 of 811 results