We narrowed to 811 results for: lars
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TypeBlog PostPublishedJan. 10, 2019, 2:38 p.m....correct phages will test. These includes labs with a large number of different phages or with phages targeting...that phage information was very fragmented. A particular phage researcher may only know how to prepare...networking Additional resources on Addgene.org Find molecular biology protocols here Visit the Addgene careers...
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CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]
TypeBlog PostPublishedFeb. 18, 2015, 3:09 p.m....production of deletions by paired DSBs may be particularly useful. The first is the study of non-coding...and Harvard Medical School. His lab studies the molecular genetics of blood cell development and disease...genomic deletion efficiency mediated by clustered regularly interspaced palindromic repeats (CRISPR)/Cas9 ... -
Who Gives a Tweet? 9 Facts About Scientists on Twitter
TypeBlog PostPublishedJune 26, 2017, 1:31 p.m....Instagram Social media and news sites were also popular. Perhaps scientists, like nonscientists, blur the...the more friends they have in common. Only the largest connected part of the follower network is shown...physical (blue) and social (orange) scientists largely cluster by discipline, suggesting that scientists... -
IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging
TypeBlog PostPublishedJune 8, 2023, 1:15 p.m.... Affinity Reagent Working Group of the Human Biomolecular Atlas Program (HuBMAP) antibody metadata standards.... Users can also recommend or not recommend a particular reagent in the given conditions, along with the...building both the community and the database. They particularly encourage people to upload and share their negative... -
6 Steps to Submitting a Resume That Gets Seen
TypeBlog PostPublishedJuly 9, 2015, 2:30 p.m....excellent resume. 2) Follow job boards and check them regularly Use your research skills to determine which job... to take next steps and follow up especially in large companies. It is fine to address the cover letter...might be able to help you. If the organization is large, you will need to be more directed and target someone... -
9 Tips to Achieve Success in Academia
TypeBlog PostPublishedApril 1, 2014, 5:33 p.m....follow their passion. “There are many ways to have a large positive impact in society. We need much, much more...studies. They suggest that you make time for extracurricular activities both to develop new skills and to...information about the benefits of pursuing extracurricular activities. Success evolves from passion True... -
Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!
TypeBlog PostPublishedJuly 18, 2017, 12:41 p.m....Robertson (far left) is a Senior Lecturer and a Plant Molecular Biology and Biotechnology expert. In order to ...engineer plants that are able to destroy DNA viruses, similar to the anti-phage CRISPR effect observed in bacteria...Nair, Associate Professor (Right), focuses on molecular biotechnology for improving expression of nutritionally... -
Tips for Improving Your Next Manuscript
TypeBlog PostPublishedNov. 22, 2016, 3:30 p.m....welcoming us. Then it was time to work! Set up similarly to the online class, we met as a group for sessions...colleagues’ manuscripts and discuss changes to particular sections. The focus groups allowed us to try ...try out techniques that were discussed in the larger sessions and reinforced our newfound knowledge with... -
Plasmids 101: Positive and Negative Selection for Plasmid Cloning
TypeBlog PostPublishedAug. 22, 2019, 12:43 p.m....plasmids at Addgene Read our molecular biology reference Watch molecular biology protocol videos ...that have lost the sacB gene. SacB can be used similarly to CcdB. For example, in the gene expression plasmid... -
Bringing Sustainable Practices to the Lab: Recycling
TypeBlog PostPublishedFeb. 14, 2019, 2:35 p.m....Anyone who works in a lab is familiar with the alarming amount of waste that is generated. This is a systemic...first post will be based on recycling! With the popularity of single-use items, plastic is a substantial... still be interested as it offsets the cost of regular waste services. Learn about how to start a glove... -
Five Questions to Ask If You're Deciding Between a Master's or PhD Program
TypeBlog PostPublishedFeb. 16, 2021, 2:15 p.m....scientist, or so I thought. So I entered a molecular and cellular biology PhD program in 2011, which I left...different however. In industry the career ladder is larger and there are more opportunities for promotion.... -
Networking and Inspiring Young Scientists at Freshers Fairs
TypeBlog PostPublishedNov. 17, 2015, 3:30 p.m.... events are one of the main ways budding young scholars across Europe get introduced to the cultures and...collaborations and conferences for their students. Another large goal of the organisation is to encourage iGEM alumni...these biological societies engaging with young scholars. We believe it’s very important to develop a network... -
CRISPR/Cas9 FAQs Answered!
TypeBlog PostPublishedMarch 13, 2014, 4:08 p.m....search for "CRISPR", short-hand for Clustered Regularly Interspaced Short Palindromic Repeats, in Pubmed...Matt Ferenc, to organize the CRISPR forum's most popular questions and answers. Here are just 4 of the CRISPR-Cas9...introduce cleavage of the target site with efficiency similar to using a single chimeric gRNA. At the same time... -
Plasmids 101: TOPO Cloning
TypeBlog PostPublishedOct. 27, 2016, 2:30 p.m....low concentration or you are cloning an extremely large insert. Since the standard ligation reaction is...Central PMCID: PMC52876. 2. Novel approach to molecular cloning and polynucleotide synthesis using vaccinia...Plasmid Cloning Protocols Catch Up On Your Basic Molecular Biology Techniques Watch Our Protocol Videos ... -
Multifunctional Neural Fibers: Modulation from Gut to Brain
TypeBlog PostPublishedAug. 31, 2023, 1 p.m....undeniably difficult connection to study on a molecular level. The brain is constantly influenced by signals...organisms, including the Anikeeva lab’s work on a similar neural fiber (Park et al., 2019). This provided...., Anikeeva, P., & Chandrakasan, A. P. (2020). Modular Optoelectronic System for Wireless, Programmable... -
Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation
TypeBlog PostPublishedApril 23, 2020, 1:15 p.m....but for viral vector core facilities producing a large number of viral preparations in parallel, there ...Single-stranded Virus Next-generation Sequencing. Molecular Therapy - Nucleic Acids 4:e260 . https://doi.org...Batch-to-Batch Consistency Evaluation of AAV Preparations. Molecular Therapy 25:1375–1386 . https://doi.org/10.1016... -
Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring
TypeBlog PostPublishedNov. 12, 2019, 2 p.m....interested in exploring biological dark matter – large tracts of the microbial tree of life that remained...IgnaviCas9 is a type II-C Cas9. IgnaviCas9 is most similar to a type II-C Cas9 from Parvibaculum lavamentivorans... incredible thermostability makes the enzyme particularly useful. Additional resources on the Addgene... -
Single-cell tracking of lineage and identity with CellTag
TypeBlog PostPublishedMay 9, 2019, 1:15 p.m....that cells with common ancestors tended to have similar reprogramming outcomes. For example, cells with...with shared CellTagD13 tags often had similar gene expression pattern changes and reprogramming outcomes...reprogramming is not stochastic, but rather there’s a particular cell state that’s permissive to reprogramming.In... -
Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource
TypeBlog PostPublishedMay 25, 2021, 2:29 p.m.... including more secure archiving, unambiguous molecular definition, and potential for further engineering...is a critical unmet need for an open-access, molecularly defined, and well validated collection. I’m thrilled..., Wong J, Koh XY, Eng H-Y, Lane D, Lefranc M-P, Clark M, Dübel S (2018) When monoclonal antibodies are... -
Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?
TypeBlog PostPublishedJune 9, 2016, 2:30 p.m....problem, Gao et al. searched for Argonaute orthologs similar to TtAgo and PfAgo, another thermophilic Argonaute...variety of mammalian cell lines, with an efficiency similar to that of Cas9. Like Cas9, NgAgo is also able ...cleavage efficiency. If this finding is replicated in larger studies, it will represent a key advantage over...