We narrowed to 629 results for: Dos;

...100percentSDS.     Additional Resources on the Addgene Blog Dos & Don'ts When Publishing a Scientific Manuscript ...introduction, etc. so as to thoroughly explain the do’s and don’ts of that week’s piece of a manuscript....

...chemically coloured lymphoid organs did in the past. How do researchers track fluorescently labeled cells? 1.... answer using two-photon microscopy include: How do T cells and/or Neutrophils travel to the sites of...Chtanova et al. 2008, Kamenyeva et al. 2015)? How do blood monocytes patrol blood vessels during immune...Auffray et al. 2007, Finsterbusch et al. 2016)? How do dendritic cells migrate to lymph nodes to activate...

... that is right for you. What type of plasmid prep do you need? As we covered in a previous post on plasmid...midiprep, etc. (more on prep sizes below). But what do we mean by DNA quality? There are a few different...starting culture conditions rather than your kit.   Do you want eco-friendly options? It’s no secret that...  While budget is an important consideration, you do want to make sure you’re getting the best bang for...

...guides as negative controls. Non-targeting guides do not target any site in the genome. This means that...confound the results of high-throughput CRISPR screens. Do short guides (17-18 bp) have less off-target cutting...have fewer off-target effects than long guides, and do not have a major reduction in on-target efficacy....

...train of action potentials that GCaMP6 and jGCaMP7 do not. Most likely, it’s a combination of many factors...supporting data and community engagement required to do this drove Addgene to develop new systems, new partnerships...resources, and collaborations, and will continue to do so, in the hopes that these findings can help others...

...site-directed knock-in. We’ll also discuss what to do if your editing efficiency isn’t as great as you ...insertion or deletion that isn’t a multiple of three will do. A popular screening method for knockouts is Tracking...base pairs modified? In these cases, it’s best to do some planning in the design phase of your experiment...

... resting phase of the cell cycle; quiescent cells do not take up nucleic acid or express transgenes as...economical mode of detection, Uphoff et. al. outline a do it yourself PCR-based approach complete with freely...Learn All About Viral Vector Elements Learn How to Do Genome-Wide Screens with CRISPR Lentiviral Vectors...

...expression levels for most purposes, but what do you do when you’ve tried a common strain and don’t get...popular commercial strains designed to avoid them. Why do I need an expression strain? Protein expression from...

...physics and sustainable design while allowing me to do my internship in the daytime.  Getting into the office...be heavy restrictions on what I could and couldn’t do as an intern. I expected that these restrictions ...

...to infect with the virus. Then the viral particles do the rest! Downstream expression or integration resulting...biological outcome you want to achieve? For example, do you want the nucleic acid cargo to be integrated ...