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We narrowed to 816 results for: LAS

Showing: 251 - 260 of 816 results

  1. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

    Type
    Blog Post
    Published
    July 28, 2015, 2:30 p.m.
    ...of the second plasmid can be done in one day in one assembly reaction. This second plasmid saves you a ... page went up last month, over 340 people have signed up to receive alerts when plasmids become available...and Myers for speaking with us! Find the Plasmids: Plasmids for protein tagging with CRISPR Additional... in many cases. In an effort to track down every last one of those gene regulatory proteins and the places... available at Addgene. For more details on the plasmids that are currently available along with detailed...system, what we are doing is adding in a second plasmid to target an epitope tag onto the transcription...second vector is a homology directed repair donor plasmid with homology arms and a selection marker. If things...

  2. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ...to a specific area at the plasma membrane (Figure 2). Just express the plasma membrane-localized iLID along...undergoes a conformational change and binds the plasma membrane under blue light CRY2-CIB1 heterodimerizes...impossible to target to specific subcellular regions, laser ablation can damage other cellular structures, and...genetic mutations are not easily reversible. Late last year, the Akhmanova Lab published a new optogenetic...module, which are also available as lentiviral plasmids if you want to make a stable cell line. This design...advantage of BcLOV4’s light-induced binding of the plasma membrane. In previous publications, they described...developing embryo is accessible for injection of plasmids and other materials.    Figure 3: Optogenetic...

  3. Our Most Popular Blog Posts from 2019

    Type
    Blog Post
    Published
    Dec. 31, 2019, 2:26 p.m.
    ... some recently deposited plasmids every quarter. The “hot plasmids” from last March were quite popular...behalf of scientists. 9. Plasmids 101: Positive and Negative Selection for Plasmid Cloning by Jennifer Tsang...start a new decade, we’re also looking back at the last year of scientific discovery. We’ve documented many...past year: CRISPR, optogenetics, chemogenetics, plasmids, fluorescent proteins, careers, and more. These...s the top 10 blog posts from the year. 10. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent... about mNeonGreen, mTFP1, and mWasabi and find plasmids containing these fluorescent proteins at Addgene..., and all possible base-to-base changes.   1. Plasmids 101: Transformation, Transduction, Bacterial Conjugation...

  4. A new platform for fast functional characterization of potentially cancerous mutations

    Type
    Blog Post
    Published
    May 7, 2019, 12:39 p.m.
    ...collection of plasmids each carrying a single gene with a defined mutation or gene fusion. Plasmids carrying...cancer research, like PIK3CA (Plasmid 116771: pHAGE-PIK3CA) and EGFR (Plasmid 116731: pHAGE-EGFR), are represented...Resources on Addgene.org Find plasmids for your research Find cancer research plasmids and resources ...mutations, and the group has deposited several hundred plasmids from this study with Addgene. Read on to learn...learn more about their work, and how these plasmids can help cancer researchers studying an assortment of...vitro approach. Using data from The Cancer Genome Atlas (TCGA), a catalog of genetic mutations responsible...well. They called this library the CTD2 FASMIC Plasmid Collection for “Functional Annotation of Somatic...

  5. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    Published
    April 5, 2018, 12:42 p.m.
    ...editing. How to use Addgene plasmids for Easi-CRISPR You can use Addgene plasmids to create a repair template...of Addgene plasmid options for templates - see the example at right using blue flame plasmid pmScarlet_...desired insert (e.g., mScarlet).   Plasmid ID Plasmid Name Tags 69090 pMJ915 MBP (N ...out our CRISPR Guide Find Plasmids for Cas9 Purification Find CRISPR Plasmids for Mammalian Expression ...pre-CRISPR was very arduous. First, you’d construct a plasmid with your desired changes, long homology arms and...the recombined product, you’d microinject into blastocysts to be implanted in pseudopregnant female mice...PAM (bold highlighted text) and cleavage sites (slashes) are shown. In this case, the green guide would...

  6. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

    Type
    Blog Post
    Published
    Oct. 30, 2018, 1:04 p.m.
    ...myogenic precursor cells [5]. Classification of stem cells Stem cells are classified into two broad types according... Visit Our Stem Cell Page Find Plasmids for Reprogramming Find Plasmids for Differentiation ... inner cell mass (ICM) of a 5-6 day old human blastocyst [6]. They are isolated mostly from abnormal and...C-MYC. iPSCs were first generated from mouse fibroblasts in 2006 in Shinya Yamanaka’s lab, where the reprogramming...successful generation of human iPSCs from dermal fibroblasts via retroviral and lentiviral infections respectively...to a Nobel Prize for Dr. Yamanaka in 2012. Find plasmids for generating iPSCs iPSCs and ESCs closely resemble...system for research in regenerative medicine over the last decade. In the next post of this series, we’ll discuss...

  7. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    Published
    Feb. 23, 2016, 3:30 p.m.
    ...PITCh-gRNA binding sites. This PITCh plasmic is cotransfected with a plasmid carrying Cas9, the PITCh-gRNA, ...Guide Pages Find CRISPR Plasmids for Your Experiments Deposit Your New Plasmid Tools with Us  ...Integration into Target Chromosomes). Using their PITCh plasmids, GFP knock-in cell lines can be created in about... knock-ins, some labs have used NHEJ for whole plasmid integration; however, since NHEJ is error-prone...cloning. An overview of PITCh. The PITCh plasmid contains a GFP-Puro cassette flanked by 5' and .... The PITCh-gRNA creates two DSBs in the PITCh plasmid to release the cassette, and an insertion-locus-specific...locus-specific gRNA The gRNA should target near the last coding exon of your gene of interest. For ideal ...

  8. Bringing Sustainable Practices to the Lab: Recycling

    Type
    Blog Post
    Published
    Feb. 14, 2019, 2:35 p.m.
    ...for an estimated 2% of global plastic generation. Less than 9% of plastics are ever recycled, so adopting...mail. Recycle plastic packaging Packaging is a huge contributor to the increase in plastic manufacturing...services don’t offer plastic recycling, specialized companies can recycle plastic-- you just have to find...other scientists save resources by sharing plasmids Find plasmids at Addgene ...recycling! With the popularity of single-use items, plastic is a substantial portion of lab waste. Despite ...manufacturing, accounting for 40% of total plastic usage (Geyer et al., 2017). While most single-stream or city ...Corning takes back and recycles a lot of their plastic packaging (from Corning®, Falcon®, or Axygen® product...

  9. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Oct. 14, 2015, 2:30 p.m.
    ...multi-subunit complex employed by class 1 systems. A putative new class 2 nuclease, Cpf1 (CRISPR from Prevotella...endonuclease of a class 2 CRISPR-Cas system." Cell (2015). PubMed PMID: 26422227. Find plasmids from this publication...: Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system. Figure from Zetsche et al.,...., 2015. How was Cpf1 found and tested? Class 2 CRISPR systems, including the type II Cas9-based system...Francisella), was annotated in several genomes and is classified as a type V CRISPR system. Like Cas9, Cpf1 contains...Francisella Cpf1 (FnCpf1), they employed an E. coli plasmid depletion assay to discover FnCpf1’s PAM sequence....1 (2016): 31-34. PubMed PMID: 27918548. Find plasmids from this publication at Addgene. 3. Kim, Daesik...

  10. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    Published
    Dec. 7, 2015, 6 p.m.
    ...CRISPR mediated genome engineering. 10. Plasmids 101: Control Plasmids Need help thinking about the types ...Scientists eBook Download the Plasmids 101 3rd Ed. eBook Read the Most Recent Plasmids 101, CRISPR 101, and Career... two eBooks (Management for Scientists and the Plasmids 101 2nd ed, the 3rd edition has now been released...top of the many developments in science and the plasmids, technologies, and researchers behind them. As...most popular posts continue to be those from our Plasmids 101 and CRISPR 101 series. These posts aim to ... the background they need to get started using plasmids and the revolutionary CRISPR technology in their...and how it’s used in CRISPR genome editing.   2. Plasmids 101: Cre-lox Cre recombinase can be used to generate...
Showing: 251 - 260 of 816 results