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We narrowed to 397 results for: organization Addgene

Showing: 251 - 260 of 397 results
  1. The time and cost required to make a plasmid

    Type
    Blog Post
    Published
    March 23, 2023, 1:15 p.m.
    ...Plasmids 101 eBook Resources on Addgene.org Join the community of Addgene depositors here Check out our...intervening five years, inflation has occurred. Here at Addgene, the consumables cost per stab has increased 50%...plasmids. Use repositories Repositories such as Addgene help share materials quickly and cheaply. We save...where to find them! Keeping good records and an organized lab saves time and money across the board. Consider...last minute experiments or changes. Fun fact: Addgene’s high-throughput approach means we virtually always...community engagement with and use of repositories like Addgene provides large-scale savings. How large? Using ...average of 5 hours of hands-on time per plasmid, Addgene, since its founding in 2004, has saved the scientific...
  2. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    Published
    Jan. 25, 2024, 2:15 p.m.
    ...Resources on Addgene.org CRISPR software and resources Addgene CRISPR Guide Addgene’s CRISPR 101 eBook...information on Class 2 Cas enzymes. The information is organized in wiki format and includes biochemical properties...end goal. CasPEDIA also features useful links to Addgene resources throughout the site. In the Experimental...each enzyme page, you'll find a link to search Addgene's collection for plasmids containing that enzyme... from the article if they're available through Addgene. So whether you're looking for the exact Cas9 plasmid... or browsing for the latest AsCas12a plasmids, Addgene and CasPEDIA have got you covered!     ... eBook More resources on the Addgene blog CRISPR 101: Which Cas9 do I choose for my CRISPR experiment...
  3. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    Published
    July 25, 2023, 1 p.m.
    ...NBK19423/ Resources on Addgene.org Addgene's most popular lentiviral plasmids Addgene's most popular retroviral...retroviral plasmids Addgene's collection of AAV plasmids Addgene’s collection of adenovirus plasmids Resources...of viral vectors here! Need Virus? Check out Addgene's Viral Service! Types of viral vectors Of the many..., typically an expression construct, to cells/organisms. This is often cDNA, shRNA, or something similar...components of adenovirus Building viral vectors Addgene has many viral vectors and helper plasmids for ...Resources on the Addgene blog Parts of the AAV packaging plasmid Overview of Lentiviral Packaging Plasmids...
  4. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    Published
    Feb. 8, 2022, 2:15 p.m.
    ...Bench setup Small adjustments in the physical organization of your workspace and your workflow can have...documents/Western%20Blotting.pdf  More resources on Addgene’s blog A Guide to Getting Started in Undergrad ...
  5. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    Published
    Feb. 13, 2024, 2:15 p.m.
    ... the effects of the drug on the actual cells or organ system. This allows scientists to get a more definitive...and current Scientist, DNA Sequencing and QC at Addgene.  References and resources References Mosch, ...
  6. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

    Type
    Blog Post
    Published
    June 25, 2019, 12:54 p.m.
    ...) is the movement of genetic material between organisms. It plays a key role in bacterial evolution and...and thus transformation efficiency. Check out Addgene’s blog to learn about making your own competent ... as well as ready-to-use viral preparations at Addgene. For more information on viral vectors, including...starting any molecular biology experiment check out Addgene’s protocol page, which has both protocols and videos...PubMed PMID: 16357262. Additional resources on the Addgene blog Read all of our Plasmids 101 blog posts Check... blog posts on plasmid cloning Resources on Addgene.org Read our protocols for plasmid cloning Find ...
  7. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

    Type
    Blog Post
    Published
    Dec. 6, 2018, 1:34 p.m.
    ...several days. In 2015, the laboratory of long-time Addgene depositor Alice Ting used yeast display-based directed...makes PL a viable method for proteomics in model organisms in which it was previously not an option, including...toehold switches to detect Zika virus (available at Addgene). The plasmids for detecting norovirus will be ...SH3 domain containing plasmids available through Addgene. These plasmids can be used to carry out any biochemical...hundreds of additional plasmids available through Addgene, for study of the of WW, PDZ, and other protein...A fundamental approach to studying membranes, organelles, and other subcellular compartments is via proteomics... Various Addgenies...
  8. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    Published
    Oct. 29, 2019, 2:52 p.m.
    ...resources on the Addgene blog: Addgene Optogenetics guide Primer on Optogenetics blog series Addgene Biosensors...patterning activities of entire tissue types or even organs. In frogs, ectopic expression of ion channels, ...discussed in Mathews and Levin, 2018) available at Addgene: MerMAID2 - A metagenomically discovered marine...
  9. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    Published
    May 26, 2020, 1:15 p.m.
    ... your CRISPR experiment was successful in your organism or cell type? You can use DNA sequencing or other...desired genomic edit.  Find SRIRACCHA plasmids at Addgene GEMCherry2, an improved monomeric fluorescent protein...stem cells.  Find GEmCherry2 reporter plasmids at Addgene Overall SRIRACHA and GEmCherry2 reporter systems.../jbc.m117.777722 Additional resources on the Addgene blog Browse all of our CRISPR blog posts Find ...with our CRISPR 101 blog posts Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids for...
  10. Developing a cold tolerant E. coli using specialized chaperones

    Type
    Blog Post
    Published
    Jan. 28, 2020, 2:35 p.m.
    ...resources on the Addgene blog Find our synthetic biology blog posts Resources on Addgene.org Find plasmids...basics.  Mesophiles vs. extremophiles Mesophilic organisms are your regular next-door microbes which grow... pC013-Twinstrep-SUMO-huLwCas13a plasmids from Addgene, which would potentially allow a stable Cas expression...” by Benoit Giquel, an outreach scientist from Addgene. The talk highlighted the importance of reagent...
Showing: 251 - 260 of 397 results