We narrowed to 792 results for: LAT

1. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

   Type

   Blog Post

   Published

   May 11, 2017, 2:30 p.m.

   ..., in principle, these experiments could find correlations between variants in a gene of poorly classified...

2. Top Requested Lentivirus and AAV of 2016

   Type

   Blog Post

   Published

   Jan. 6, 2017, 3:56 p.m.

   ...CRISPR tools, though we do provide a few non-CRISPR related viral preps. Cas9 is the headliner of most CRISPR...

3. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

   Type

   Blog Post

   Published

   June 25, 2019, 12:54 p.m.

   ... they can be made competent through chemical manipulation in the lab. This is commonly done using calcium...

4. Communicating Your Science With Help From ComSciCon

   Type

   Blog Post

   Published

   July 19, 2018, 1:12 p.m.

   ... it.  In the lab, it might mean persuasively articulating why the results you've measured should change...

5. How Addgene Creates Content

   Type

   Blog Post

   Published

   Nov. 17, 2022, 2:15 p.m.

   ...researchers in creating and sharing knowledge, related to the types of materials we help distribute and...

6. AAV Q&A with Tim Miles

   Type

   Blog Post

   Published

   May 17, 2022, 1:15 p.m.

   ...elusive.  There has also been a lot of effort in immunomodulation or immune suppression to allow AAV re-administration...

7. Antibodies 101: Buffers, Storage, and Conjugates

   Type

   Blog Post

   Published

   Feb. 1, 2022, 2:15 p.m.

   ...can be very helpful if you're trying to find a relatively rare protein in an experiment with many proteins...

8. How to Keep a Lab Notebook for Bioinformatic Analyses

   Type

   Blog Post

   Published

   June 2, 2016, 2:30 p.m.

   ...TextWrangler to record the following, which are later printed for my physical lab notebook, along with...

9. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

   Type

   Blog Post

   Published

   Feb. 24, 2015, 7:50 p.m.

   ...theoretical limit on the number of genes that can be manipulated at one time. Weissman’s group has made standard...

10. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type

    Blog Post

    Published

    March 3, 2020, 2:15 p.m.

    ...and further studies of optical neural circuit manipulations when combined with existing green-fluorescent...

11. RANbodies: Reporter Nanobody Fusions

    Type

    Blog Post

    Published

    April 10, 2018, 12:56 p.m.

    ...individual labs to complete. The nanobody based RANbody platform from the Sanes Lab overcomes this limitation and...

12. Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute

    Type

    Blog Post

    Published

    Feb. 2, 2018, 3:20 p.m.

    ...certificate in Strategic Communications and Public Relations at the University of Washington and the connections...

13. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type

    Blog Post

    Published

    June 15, 2017, 2:30 p.m.

    ...is steadily increasing. Of course, you want the ‘latest and greatest’ variants with the best properties...

14. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type

    Blog Post

    Published

    Dec. 8, 2016, 3:30 p.m.

    ...for non-cytotoxic proteins which retain their regulatory functions.” Mol Microbiol. 1995 Mar;15(6):1031...

15. Reproducibility for Everyone: Lessons from an Open Science Collaboration

    Type

    Blog Post

    Published

    Feb. 28, 2019, 1:34 p.m.

    ...specific audience. For example, I joined this effort later in the year and in the fall of 2018, I helped organize...

16. Celebrating 15 Years of Scientific Sharing

    Type

    Blog Post

    Published

    Jan. 14, 2019, 2:08 p.m.

    ... succeed.  Thanks to all the Addgenies and congratulations to current and alumni Addgenies on our success...

17. Summer SciComm Series: Cognitive Load

    Type

    Blog Post

    Published

    June 16, 2022, 1:15 p.m.

    ...require any detailed retention of information or formulation of new knowledge than it is to read a manuscript...

18. Transferable Skills Guide: Problem Solving

    Type

    Blog Post

    Published

    Nov. 30, 2021, 2:15 p.m.

    ...able to recognize it as a part of your skill set later on. If you are new to research and the lab, you ...

19. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

    Type

    Blog Post

    Published

    May 11, 2021, 1:15 p.m.

    ...Optogenetics and chemogenetics are powerful tools to modulate the activity of neurons and other brain cells....

20. 5 Steps to Writing Grant Proposals for Grad Students

    Type

    Blog Post

    Published

    Feb. 11, 2014, 12:13 p.m.

    ...attention to the keywords used and think of ways to relate the proposal and deliverables to the aims requested...

21. How to Prepare for an Industry Interview

    Type

    Blog Post

    Published

    July 9, 2020, 1:15 p.m.

    ...Congratulations, you’ve just landed an interview for an industry job! You’ve worked hard to earn this...

22. Progress Towards a PAM-Free CRISPR

    Type

    Blog Post

    Published

    March 7, 2024, 2:39 p.m.

    ...SpG Cas9 with the same PAM preference of NG was isolated by the Kleinstiver Lab (read more on this lab'...

23. High Complexity Golden Gate Assembly with NEB’s DAD

    Type

    Blog Post

    Published

    Aug. 1, 2023, 1:15 p.m.

    ...researchers to select large sets (with overhangs that violate some of the traditional design rules) for successful...

24. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type

    Blog Post

    Published

    March 8, 2017, 3:15 p.m.

    ...Other” column. For example, you may enter “donor template vector”. Cloning Method Choose from: Gateway...

25. Science communication: 9 Strategies to Get Your Foot in the Door

    Type

    Blog Post

    Published

    Sept. 27, 2017, 1:23 p.m.

    ...-scientists in an unusual setting: Discuss your latest research in a local pub at a Pint of Science event...

26. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type

    Blog Post

    Published

    May 3, 2022, 1:15 p.m.

    ...fragment An enhanced recombinant amino-terminal acetylation system and novel in vivo high-throughput screen...

27. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

    Type

    Blog Post

    Published

    May 4, 2021, 1:15 p.m.

    ...chain and light chain for the antibody and when translated into protein will assemble into a fully functional...

28. Viral Vectors 101: Producing Your rAAV

    Type

    Blog Post

    Published

    July 16, 2024, 1:15 p.m.

    ...purified secreted rAAV for rapid and cost-effective manipulation of gene expression in the CNS. Molecular Neurodegeneration...

29. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type

    Blog Post

    Published

    Sept. 24, 2015, 2:30 p.m.

    ...want to include a homology directed repair (HDR) template to create knock-ins, point mutations, or to add...

30. DNA Purification Without a Kit

    Type

    Blog Post

    Published

    Feb. 11, 2020, 2:15 p.m.

    ...tendency to runout right when you need them and to accumulate a bunch of unused buffers because you’ve run ...