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Showing: 501 - 520 of 569 results

  6. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ... neuron can then be traced throughout the brain based on its unique hue (plasmids at Addgene). A large-scale...Its efficiency is comparable to conventional dye-based tracing while also having the added advantage of...

  8. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    Published
    April 20, 2021, 2:30 p.m.
    ... in the envelope and are targets for complement-based killing by anti-alphaGal antibodies. The VSV-G envelope...

  9. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    Published
    Aug. 20, 2020, 2:30 p.m.
    ...genomic edit. Some Cas13 enzymes require a single base protospacer flanking sequence (PFS) adjacent to ...from Staphylococcus aureus (SaCas9) is about 1 kilobase smaller than SpCas9, so it can be packaged into...

  12. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ...should first develop primers to the approximately 500 base pair sequence surrounding your gRNA. Once amplified...

  14. March for Science

    Type
    Blog Post
    Published
    April 21, 2017, 2:30 p.m.
    ... the meaning of the word ‘fact’. Policy must be based on our most accurate interpretation of the world...

  15. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    Published
    June 23, 2016, 2:30 p.m.
    ...fail to recapitulate the true steric and charge-based nature of the phosphoryl-modification; they are ...appreciated. PhosphoSitePlus, one of the most inclusive databases of post-translational modifications, identifies...

  16. Antibodies 101: Validation

    Type
    Blog Post
    Published
    March 24, 2022, 1:15 p.m.
    ...same conditions. Example: You want to sort cells based on the expression of a new receptor that is of interest...attempts by other means, such as through antibody databases like Antibodypedia or through your antibody provider...

  17. Hot Plasmids: Summer 2024

    Type
    Blog Post
    Published
    Aug. 27, 2024, 1:15 p.m.
    ...Genome-wide programmable transcriptional memory by CRISPR-based epigenome editing. Cell, 184(9), 2503–2519.e17. ...

  18. Finding Your Science Policy Path

    Type
    Blog Post
    Published
    Sept. 22, 2020, 1:15 p.m.
    ...Think a university system who develops evidence-based university policy to combat sexual harassment in...
Showing: 501 - 520 of 569 results