We narrowed to 583 results for: BASE

...CREATE method?  CREATE stands for Cre Recombinase-based AAV Targeted Evolution. In this technique, a pool...

...Learn about Non-Homologous End Joining Learn about Base Editing Read 3 Tips to Improve HDR Efficiency for...

...appropriately credited, broadening the knowledge base of AAV vector performance in various brain regions...

...community obtained high-quality data for our DNA-based reagents. Not only do we extract as much information...we routinely wrote Python scripts, queried our database with SQL or Django, and executed code in Jupyter...

...desk jobs “alternative careers.” Realistically, based on the numbers of PhDs and available positions alone...

...Garcia-Marcos’ lab recently developed a collection of BRET-based ONE vector G-protein Optical (ONE-GO) biosensors...

...could be harnessed or enhanced for future MagLOV-based tools, just as light-induced conformational changes...

...simplicity we will discuss the more common enzyme-based reporter methods. For enzymatic signal detection...

...Lentiviral Vectors for Delivery of Gene-Editing Systems Based on CRISPR/Cas: Current State and Perspectives. Viruses...

...followed by sequencing and further data analysis. Based on Figure 31.5.2 from McDade et al., 2016. ...

...over the other BiFC is a convenient, fluorescence-based method for directly visualizing PPIs in cells. The...

...development called Scrum. The Scrum framework is based on an iterative and collaborative approach to development...

...particularly important if your amplicon is longer than 150 base pairs. While we use ddPCR for AAV titration, there...

...resources towards the development of a new therapy based on its market need and anticipated commercial success...

... 1a). Other strategies that have been developed based off of this concept include: Self-labeling tags...

...Ohashi, T., et al., An experimental study of GFP-based FRET, with application to intrinsically unstructured...

...whether you will create an N- or C-terminal fusion based on your experimental needs. Equally important, before...

... it, could allow idea generation and extension, based on existing, published data. The importance of data...

... neuron can then be traced throughout the brain based on its unique hue (plasmids at Addgene). A large-scale...Its efficiency is comparable to conventional dye-based tracing while also having the added advantage of...

... in the envelope and are targets for complement-based killing by anti-alphaGal antibodies. The VSV-G envelope...