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We narrowed to 847 results for: CAL;

Showing: 501 - 520 of 847 results

  1. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    Published
    June 12, 2018, 11:40 a.m.
    ...to think about DNA in this context, but also specifically study it. They might be researching enhancer...--directly or indirectly--many other areas of biological research. If you’re looking for resources that...you study how chromatin effects your favorite biological phenomenon, there’s a reasonable good chance ...RANbodies) as versatile, small, sensitive immunohistochemical reagents. Yamagata M, Sanes JR Proc Natl ...

  2. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    Published
    June 6, 2023, 1:15 p.m.
    ...upon infecting a host cell. This viral DNA (also called proviral DNA) is then inserted into the host's ...human gene therapy and is currently being used in clinical settings. AAV is also a cool tool in the laboratory...that we’ve reviewed the common viruses used in biological research, you might have an idea which ones could...Introduction to AAV Viral Vectors 101: Viruses as Biological Tools ...

  3. Antibodies 101: ChIP

    Type
    Blog Post
    Published
    March 10, 2022, 3:25 p.m.
    ...your sample before moving on to the next step. Typically, this can be done by cutting the tissue sample...into random, small pieces of roughly equal size, typically between 200-1000 bp. This is to ensure that your...2) fragmented the DNA (3) used antibodies to specifically select for proteins of interest (4) isolated... be a good idea to add "patience and an extra-methodical approach" to the top of your protocols when embarking...

  4. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    Published
    June 29, 2021, 1:15 p.m.
    ...1:1 ratio and then cultured in a special medium called HAT. This medium selects against unfused myeloma...antibody, meaning that the antibodies are largely identical between lots.  Disadvantages of monoclonal antibodies...there will be changes between lots over time. Specifically, the antigen-binding sites of the antibody -...2016) Ligand-induced Epitope Masking. Journal of Biological Chemistry 291:20993–21007. https://doi.org/10.1074...

  5. Developing a cold tolerant E. coli using specialized chaperones

    Type
    Blog Post
    Published
    Jan. 28, 2020, 2:35 p.m.
    ...Following up its 2018 iGEM debut - centered around a prototypical methane biosensor - IISER Bhopal is back in ...bacteria. These bacteria inhabit cold temperature locales and psychrophilic enzymes help them survive under...forced to express in mesophilic host systems. The so-called “regular” temperatures of growth, say around 37... from their native conditions of ~0°C, which drastically affects their stable folding and efficient functioning...

  6. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    Published
    June 2, 2015, 6:06 p.m.
    ...Multiplexing is key due to the natural redundancy of biological pathways;  to observe a phenotype, the modification.... Though the plasmids made by Xie et. al are specifically designed for plant expression, PTGs will likely...likely be adaptable to many different biological systems. Although Xie et. al characterized the system ... PTGs will likely work well in many different biological systems. Size is also a factor; the pol lll promoter...

  7. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

    Type
    Blog Post
    Published
    July 10, 2014, 7:17 p.m.
    ... Ventures – a local Cambridge biotech venture capital firm with deep ties into the local academic community... As a biomedical engineer and scientist, I like to envision that my research will have a direct impact...communication styles in academia and industry vary drastically, he reassures that one can learn it by reading...

  8. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type
    Blog Post
    Published
    Feb. 18, 2014, 4:22 p.m.
    .... Listen to our podcast interview with Harvard Medical School researcher Connie Cepko to learn about her...mentoring programs. The groups in these programs typically consist of 1-2 mentors and 3-5 mentees who meet...present formally often enough) and give honest, critical feedback for improvement. Go to a science or career...their career paths and get their advice. Attend a local networking event together and help each other practice...

  9. Will You Be My Mentor? Finding and Asking for Mentoring Support

    Type
    Blog Post
    Published
    Feb. 4, 2014, 3:50 p.m.
    .... Listen to our podcast interview with Harvard Medical School researcher Connie Cepko to learn about her... work product in action) Can help you learn the local “ropes” and become an insider faster A mentor that...chapters. Find out what other groups have going on locally. Your university probably has an alumni directory...As a future alum, these are people you can “cold call” and ask for some of their time. There are many ...

  10. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ...and can be specifically targeted to a number of different cell types even when systemically administered...with maximum “trunk” space for genetic cargo. Typically, when researchers speak about AAVs, they are actually...ITRs and serotypes The names of AAVs contain two numerical designations, separated by a slash, that indicate...

  11. To Each HIS Own

    Type
    Blog Post
    Published
    March 2, 2023, 2:15 p.m.
    ...Much of today's biological research requires a close examination of specific proteins within a system...without eluting the target protein and needs to be empirically determined. In addition, some metal ion substrates...function. B, The string of histidines in the tag is typically between 6 to 9 residues long. The small size of...Enfors SO, Persson I, Nilsson B and Uhlen M. Large-scale affinity purification of human insulin-like growth...

  12. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    Published
    Feb. 16, 2023, 7:05 p.m.
    ...protein expression in mammalian cells: easy to use biological tools are abundant! In this post, we will review...that! CRISPR interference (CRISPRi) targets a catalytically dead Cas9 fused to a transcriptional repressor... protein, then these systems need to be site-specifically knocked in. This can be labor intensive and ...and target-specific protein degradation. Nature Chemical Biology. May 2018. 14(5): 431-441. PubMed PMID...

  13. Summer SciComm: Preprints

    Type
    Blog Post
    Published
    Aug. 16, 2022, 1:15 p.m.
    ...arXiv.org), but have been only adopted by biological and biomedical scientists (see: bioRxiv.org or medRxiv.org...Preprints can be read freely by anyone and are typically free of charge for authors to post. They can be... be assessed and commented on by readers, typically peers in the field, and updated by the authors over...

  14. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    Published
    March 15, 2022, 1:15 p.m.
    ...exciting new project aimed at understanding how a critical cellular pathway is regulated. You’ve read all...do you know about the vendor and the vendor’s technical support? An antibody from a vendor that you use...frequently, with a responsive and knowledgeable technical support team, is a safer bet than one from a large... all validation is equal and you should think critically about what data is and is not shown on an antibody...

  15. RNA Extraction Without A Kit

    Type
    Blog Post
    Published
    March 24, 2020, 1:15 p.m.
    ...widely known of these products is TRIzol® (also called TRI Reagent®, RNAzol®, QIAzol®  and sold by many... within cells or tissues- they don’t actually chemically stabilize the RNA molecules (Allewell and Sarma... MacDonald RJ, Rutter WJ (1979) Isolation of biologically active ribonucleic acid from sources enriched...Guanidinium Thiocyanate–Phenol–Chloroform Extraction. Analytical Biochemistry 162:156–159. https://doi.org/10.1016...

  16. Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute

    Type
    Blog Post
    Published
    Feb. 2, 2018, 3:20 p.m.
    ...because her skill set wasn’t matching up with the local job postings. “There were jobs that I didn’t seem...team meetings and Susan is assigned to cover the Clinical Research Division at Fred Hutch, so she attends...work include: “Listening. Evaluating evidence. Critical thinking. Being able to learn things quickly. ...like she did for the Northwest Association of Biomedical Research. She does, however, recommend using ...

  17. Reproducibility for Everyone: Lessons from an Open Science Collaboration

    Type
    Blog Post
    Published
    Feb. 28, 2019, 1:34 p.m.
    ... helped by expanding this team, leading monthly calls, and planning next steps. April Clyburne-Sherin ...first iteration of these workshops took place at Caltech in Jan 2018 and involved presentations and a handout...Not everyone in the group was able to make it physically to instruct the workshop at the conference, but...have the materials and resources to run their own local workshops was a major goal for the team last year...

  18. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

    Type
    Blog Post
    Published
    Dec. 16, 2015, 3:30 p.m.
    ... Not only is CRISPR having effects on various biological disciplines, the base technology itself is constantly...at non-target sites. Means to decrease these so-called “off-target effects” have included using a Cas9... the Broad Institute, the Joung lab at Harvard Medical School, and the Doudna Lab at Berkeley set out ...afforded by gRNA binding at non-target sites would theoretically decrease off-target cutting. Figure 1...

  19. A Look at Addgene's QC Process

    Type
    Blog Post
    Published
    May 6, 2025, 1:15 p.m.
    ...plasmids. These are prone to error — both human and biological. Even the most high-fidelity polymerases can ...the correct inserts, tags, promoters, and other critical functional components. Fun fact! Some older plasmids...sequences. These sequences can also be confirmed or theoretical. No matter the level of information that is provided...the raw NGS reads and reassemble using a program called Geneious. Here, the raw reads are mapped against...

  20. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    Published
    April 6, 2023, 3 p.m.
    ...choosing antibodies   Complexity is a key factor Biological systems tend to be complex with a variety of ...multiple targets in parallel. In these cases, it is critical that users choose antibodies that are easily distinguishable...staining multiple targets indirectly, you must strategically plan primary/secondary antibody pairing such...Fisher, S. K. (1993). Chapter 15 Postembedding Immunocytochemical Techniques for Light and Electron Microscopy...
Showing: 501 - 520 of 847 results