We narrowed to 568 results for: CHI
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TypeBlog PostPublishedJune 5, 2014, 3:32 p.m....markets), and serious ideas (how to stop rhino horn poaching using biotechnology) were thrown on the table ...
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No Llamas Required - Synthetic Nanobodies Against Membrane Proteins
TypeBlog PostPublishedJune 18, 2020, 1:15 p.m.... Torres GM, Wang N, Van Breedam W, Roose K, van Schie L, Hoffmann M, Pöhlmann S, Graham BS, Callewaert... -
Cloning Mammalian Cells with the Agarose Method
TypeBlog PostPublishedSept. 7, 2017, 12:17 p.m....transfection efficiency is not known, this is easily achieved by splitting transfected cells into 3 plates at... -
Plasmids 101: 5 factors to help you choose the right cloning method
TypeBlog PostPublishedAug. 21, 2018, 12:31 p.m.... of the post! Speed: Your grant deadline is approaching or your advisor is demanding results. If you’... -
10 Basic tips for mammalian cell culture
TypeBlog PostPublishedJuly 12, 2018, 1:09 p.m..... Additional passages will allow your cells to achieve a consistent growth rate. For every minute spent... -
xCas9: Engineering a CRISPR Variant with PAM Flexibility
TypeBlog PostPublishedMarch 28, 2018, 6:52 p.m....fused to third generation cytidine base editor architecture (BE3) slightly improves NGG PAM editing efficiency... -
Hot Plasmids and Viral Preps - May 2021
TypeBlog PostPublishedMay 18, 2021, 1:15 p.m....these therapeutics. Jesse Bloom’s lab at the Fred Hutchinson Cancer Research Center developed a pooled plasmid... -
Hot Plasmids: Winter 2025
TypeBlog PostPublishedMarch 11, 2025, 1:15 p.m....inherited the genomic edit but not the editing machinery. Together, these advances have the potential to... -
The AAVantages of AAV in CRISPR Screens
TypeBlog PostPublishedFeb. 4, 2025, 2:15 p.m....vectors can be made at high titers equal to those achieved with lentivirus but offer an improved safety profile... -
Antibodies 101: Flow Compensation
TypeBlog PostPublishedMay 16, 2024, 1:15 p.m.... Inputting compensation values differs between machines or the program you’re using, but usually you can... -
Anatomy of a Plasmid Page at Addgene
TypeBlog PostPublishedFeb. 4, 2016, 3:30 p.m....know the catalog number of the plasmid you are searching for, this is the quickest and easiest way to find... -
Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards
TypeBlog PostPublishedMay 22, 2015, 4:58 p.m....dengue for many years. Since more than a thousand children died of malaria today, let's not risk a potential... -
Tips for Using BLAST to Verify Plasmids
TypeBlog PostPublishedMay 29, 2014, 1:29 p.m....sequences and may be the most comprehensive for searching. Timesaving Tip #1: If you know the species that... -
DNA Purification Without a Kit
TypeBlog PostPublishedFeb. 11, 2020, 2:15 p.m....method from the Bishop Lab at the University of Chicago doesn’t use actual milk to purify DNA, but rather... -
MXS Chaining
TypeBlog PostPublishedFeb. 7, 2017, 3:30 p.m.... 3. Gibson DG, Young L, Chuang RY, Venter JC, Hutchison CA, Smith HO.Enzymatic assembly of DNA molecules... -
Transferable Skills Guide: Identifying Your Transferable Skills
TypeBlog PostPublishedJan. 11, 2018, 3:29 p.m....technical expertise in a specific field. While searching for jobs and making connections, avoid falling... -
Negative Can Be Positive: Open AAV Data with Addgene
TypeBlog PostPublishedSept. 6, 2022, 1:15 p.m....214–225. https://doi.org/10.1089/hgtb.2019.105 Rumachik, N. G., Malaker, S. A., Poweleit, N., Maynard,... -
Deep Dive: Fixing and Permeabilizing for Immunofluorescence
TypeBlog PostPublishedAug. 30, 2022, 1:15 p.m....the accessibility of your target epitope(s). To achieve these goals, researchers commonly use one of two... -
Plasmids 101: Choosing an Antibiotic Resistance Gene
TypeBlog PostPublishedOct. 10, 2023, 1 p.m...., P., & Berkmen, M. (2012). SHuffle, a novel Escherichia coli protein expression strain capable of correctly... -
Viral Vectors 101: The Retroviral Lifecycle
TypeBlog PostPublishedJuly 27, 2023, 1:15 p.m....into the host cell’s genome and then use cell machinery for transcription and translation. Here, we will...