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Showing: 501 - 520 of 620 results

  2. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...invaluable resource. I really appreciate the work you do to make sharing reagents and protocols so easy!” ...

  3. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    Published
    Dec. 8, 2022, 2:15 p.m.
    ...resources on the Addgene Blog CRISPR 101: Which Cas9 do I choose for my CRISPR experiment? The PAM requirement...

  6. Antibodies 101: Conjugation

    Type
    Blog Post
    Published
    March 26, 2024, 1:15 p.m.
    ... unconjugated version, but if you find yourself doing your own conjugation, it is important to keep in...

  9. Human Germline Editing Using CRISPR

    Type
    Blog Post
    Published
    Aug. 10, 2017, 2:19 p.m.
    ...diagnosis (PGD) screens embryos to find those that do not carry a given dominant disease-causing gene, ...

  10. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    Published
    April 28, 2016, 2:30 p.m.
    ...off"). These Cre-off switches may be referred to as DO (Double-floxed Orientation) and could be used to ...

  15. A Look at Addgene's QC Process

    Type
    Blog Post
    Published
    May 6, 2025, 1:15 p.m.
    .... Fun fact! Some older plasmids in our collection do not have a full sequence, as they were deposited ...

  16. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    Published
    Aug. 29, 2024, 1:15 p.m.
    ...Doench, “Whole plasmid sequencing is just part of doing good science!” Find the Fragmid kit here!  Cloning...

  17. Plasmids 101: Protein Expression

    Type
    Blog Post
    Published
    June 7, 2018, 1:17 p.m.
    ...Despite their advantages, mammalian expressions systems do require demanding cell culture conditions compared...

  20. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    Published
    Nov. 7, 2017, 1:59 p.m.
    ...been used to treat 10 infants with this disease. To do so, their T-cells were grown in vitro, their mutations...to be untreatable until 2008. Following a single dose of subretinal administration of rAAV2 carrying the...
Showing: 501 - 520 of 620 results