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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 623 results for: Dos;

Showing: 501 - 520 of 623 results

  2. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    Published
    April 16, 2015, 3:45 p.m.
    ... ends better substrates for ligation. Although we do not describe these steps here, the processing of ...

  3. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...Have you used the Cre-lox system in your research? Do you have any tips or tricks, or clever cre-dependent...

  7. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...invaluable resource. I really appreciate the work you do to make sharing reagents and protocols so easy!” ...

  8. Human Germline Editing Using CRISPR

    Type
    Blog Post
    Published
    Aug. 10, 2017, 2:19 p.m.
    ...diagnosis (PGD) screens embryos to find those that do not carry a given dominant disease-causing gene, ...

  10. Antibodies 101: Conjugation

    Type
    Blog Post
    Published
    March 26, 2024, 1:15 p.m.
    ... unconjugated version, but if you find yourself doing your own conjugation, it is important to keep in...

  11. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    Published
    Dec. 8, 2022, 2:15 p.m.
    ...resources on the Addgene Blog CRISPR 101: Which Cas9 do I choose for my CRISPR experiment? The PAM requirement...

  13. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    Published
    April 28, 2016, 2:30 p.m.
    ...off"). These Cre-off switches may be referred to as DO (Double-floxed Orientation) and could be used to ...

  17. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    Published
    Aug. 7, 2018, 12:31 p.m.
    ... provided you with a few ideas to consider if you do run into a roadblock and encourage you to check out...overcome this issue, we recommend using a range of doses of lentivirus when transducing a target cell line...transduction experiment. Media choice, reaction vessel, dose of virus, volume and timing of transduction will...

  18. Plasmids 101: Protein Expression

    Type
    Blog Post
    Published
    June 7, 2018, 1:17 p.m.
    ...Despite their advantages, mammalian expressions systems do require demanding cell culture conditions compared...
Showing: 501 - 520 of 623 results