We narrowed to 553 results for: ESC
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TypeBlog PostPublishedMay 29, 2014, 1:29 p.m....matrix BLOSUM62, but you may want to check the description of the other matrices to see if another would...
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New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries
TypeBlog PostPublishedOct. 4, 2018, 12:44 p.m.... signal amplification in gene expression and fluorescence imaging." Cell 159.3 (2014): 635-646. PubMed... -
Negative Can Be Positive: Open AAV Data with Addgene
TypeBlog PostPublishedSept. 6, 2022, 1:15 p.m....standards (clear and detailed methods and image descriptions, a minimum n = 2, and well labeled high- and... -
Plasmids 101: Choosing an Antibiotic Resistance Gene
TypeBlog PostPublishedOct. 10, 2023, 1 p.m...., P., & Berkmen, M. (2012). SHuffle, a novel Escherichia coli protein expression strain capable of correctly... -
An Introduction to Adenovirus
TypeBlog PostPublishedOct. 3, 2023, 1:15 p.m....Early 3) genes deleted. These genes’ functions are rescued by helper plasmids used during production and/or... -
Viral Vectors 101: The Retroviral Lifecycle
TypeBlog PostPublishedJuly 27, 2023, 1:15 p.m....is a turn of phrase — “pseudotransduction” — to describe the direct protein transfer of RT, which would... -
Course-Based Undergraduate Research Experiences (CUREs)
TypeBlog PostPublishedFeb. 9, 2023, 2:15 p.m....Histidine tag, malate dehydrogenase, and green fluorescent protein. Biochemistry and Molecular Biology Education... -
CRISPR 101: Making a Knock-In Cell Line
TypeBlog PostPublishedDec. 22, 2022, 2:15 p.m....diving cells will have higher HR frequencies than quiescent cells. It is important to know that HR is often... -
What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry
TypeBlog PostPublishedJuly 1, 2025, 1:15 p.m....need to use a complementary assay, such as a fluorescence based assay or running an agarose gel. In the... -
DNA Purification Without a Kit
TypeBlog PostPublishedFeb. 11, 2020, 2:15 p.m....plasmid purification and DNA gel extraction method described in Kim and Morrison is nearly identical to that... -
A Guide to Starting Your Own Journal Club
TypeBlog PostPublishedFeb. 6, 2020, 3 p.m....served. I hope that with some of the information described above you feel empowered to start your own journal... -
Lentiviral Vector Uses and Overview
TypeBlog PostPublishedMay 19, 2016, 2:30 p.m....Fusil F, Down J, Denaro M, Brady T, Westerman K, Cavallesco R, Gillet-Legrand B, Caccavelli L, Sgarra R, ... -
Plasmids 101: Colony PCR
TypeBlog PostPublishedMay 12, 2016, 2:30 p.m....expected results for the three primers previously described. When using insert-specific primers (1), positive... -
Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus
TypeBlog PostPublishedMarch 17, 2016, 2:30 p.m....minigenome system Minigenome systems, such as the one described here, are extremely useful research tools. In ... -
The Importance of a Fun Workplace: Company Culture at Addgene
TypeBlog PostPublishedSept. 3, 2015, 2:30 p.m.... ordinary environment Team building events like Escape the Room or Habitat for Humanity take you and your... -
Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View
TypeBlog PostPublishedOct. 19, 2017, 1:54 p.m....vectors are the solution. Flex vectors expressing fluorescent cytoplasmic markers (e.g. eYFP) and synaptic ... -
Plasmids 101: Gateway Cloning
TypeBlog PostPublishedJan. 12, 2017, 3:30 p.m....Gateway technology relies on the two reactions described below: The BP Reaction takes place between the... -
Overcoming the AAV Size Limitation for CRISPR Delivery
TypeBlog PostPublishedSept. 16, 2020, 1 p.m....as potent and specific? The two AAV strategies described above showed successful target modification, indicating... -
Plasmids 101: Stringent Regulation of Replication
TypeBlog PostPublishedJune 20, 2024, 1:15 p.m....replicate—but not always to the same extent. As described in our previous Origin of Replication post, DNA... -
Bioinformatics at Addgene
TypeBlog PostPublishedJuly 14, 2022, 1:15 p.m....of data. In this two-part blog series, we will describe the importance of bioinformatics at Addgene and...