We narrowed to 530 results for: Erf

1. Transferable Skills Guide: Time Management

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   Blog Post

   Published

   Dec. 5, 2017, 1:52 p.m.

   ...quick and useful tasks will swiftly give you the wonderful feeling of accomplishment while the longer tasks...

2. Deep Mutational Scanning with One Pot Saturation Mutagenesis

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   Blog Post

   Published

   Feb. 22, 2017, 3:30 p.m.

   ...ease of use, 2) time savings, and 3) ability to perform single site or multi-site saturation mutagenesis...

3. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

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   Blog Post

   Published

   April 9, 2019, 1:13 p.m.

   ...tagged protein because of its size. This type of interference is known as steric hindrance. For instance, ...

4. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

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   Blog Post

   Published

   March 5, 2019, 1:08 p.m.

   ... intestinal crypt, and since then has become a powerful tool for understanding cell proliferation. Inducible...

5. Transferable Skills Guide: Conflict Resolution

   Type

   Blog Post

   Published

   Jan. 4, 2022, 2:15 p.m.

   ...form of (hopefully constructive) conflict. As you perform the experiments to address a reviewer’s concerns...

6. Antibodies 101: Single Chain Fragment Variables (scFvs)

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   Blog Post

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   June 3, 2021, 1:15 p.m.

   ...functional scFvs; however, some individual scFvs performing better in one configuration than the other (Sandomenico...

7. New Optogenetic Tools for Cytoskeleton and Membrane Control

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   Blog Post

   Published

   Sept. 28, 2023, 1:15 p.m.

   ...recruiting proteins to the cell membrane isn’t powerful enough, what about altering the chemical composition...

8. Tips for Getting a Faculty Position

   Type

   Blog Post

   Published

   July 20, 2023, 1:15 p.m.

   ...weeds. Check out Joanne Kamens's "10 Steps to a Perfect Science Talk." 4. The chalk talk, simple in principle...

9. An Integrin Antibody Toolkit from IPI

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   Blog Post

   Published

   March 28, 2024, 1:15 p.m.

   ...integrins at non-ligand-binding sites and do not interfere with integrin function. Overall, the synthetic...

10. CRISPR 101: Homology Directed Repair

    Type

    Blog Post

    Published

    Jan. 26, 2023, 2:15 p.m.

    ...targeted-break technology coupled with HDR is a powerful site-specific knock-in tool that can be harnessed...

11. Plasmids 101: Biotinylation

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    Blog Post

    Published

    Nov. 15, 2018, 1:50 p.m.

    ...relatively small, water soluble molecule that does not interfere with the macromolecules it is added to. Biotin...

12. Using Phosphoserine to Study Protein Phosphorylation

    Type

    Blog Post

    Published

    June 23, 2016, 2:30 p.m.

    ...generate non-phosphorylated recombinant protein and perform in vitro kinase reactions. Often, however, researchers...

13. Evolution of Lab Techniques

    Type

    Blog Post

    Published

    June 21, 2016, 2:30 p.m.

    ...upon the incorporation of a ddNTP. Researchers perform four different reactions, one for each radiolabelled...

14. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

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    Blog Post

    Published

    March 1, 2016, 3:30 p.m.

    ... restriction site for linearization. This is a perfect case for the use of Gibson assembly combined with...

15. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type

    Blog Post

    Published

    April 24, 2015, 2:39 p.m.

    ...of the  Brainbow multicolor labeling system - a perfect example of the continued utility of Cre-lox. Check...

16. Plasmids 101: Common Lab E. coli Strains

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    Blog Post

    Published

    Nov. 7, 2014, 2:56 p.m.

    ...gene of interest, painstakingly cloned into the perfect empty backbone, made sure to add the right tags...

17. Is this the right place for me? 8 tactics for choosing a lab

    Type

    Blog Post

    Published

    Oct. 2, 2018, 12:56 p.m.

    ... Or is it teamwork that brings out your best performance and learning? Everyone’s different so give some...

18. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type

    Blog Post

    Published

    Jan. 16, 2025, 2:15 p.m.

    ...Michael, S., Kuleshova, E., Ojala, D., Huang, C.-C., Gerfen, C. R., Schiller, J., Dudman, J. T., Hantman, A...

19. Viral Vectors 101: AAV Variables That Matter

    Type

    Blog Post

    Published

    June 1, 2023, 1:15 p.m.

    ...first amount that looks effective. Ultimately, the perfect “high titer” is one that optimizes gene expression...

20. When Fidelity Matters: A frank discussion about ligase fidelity

    Type

    Blog Post

    Published

    March 6, 2018, 1:49 p.m.

    ...polynucleotide ligase and RNA capping enzyme superfamily of covalent nucleotidyltransferases." Current...