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We narrowed to 809 results for: LAS

Showing: 501 - 520 of 809 results

  1. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    Published
    Feb. 21, 2019, 1:15 p.m.
    ...specific mutations in your plasmids Use REPLACER Mutagenesis to mutate your plasmid Resources on Addgene.... targeted mutagenesis experiments? Find EvolvR plasmids at Addgene! References Gaj, Thomas, and Pablo...Browse our CRISPR guide pages Find bacterial CRISPR plasmids ...

  2. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    Published
    June 6, 2023, 1:15 p.m.
    ...their required components are very similar. While plasmids containing virus-specific elements such as the...integrate, and the addition of a helper virus or plasmid allows it to replicate episomally, essentially ...adenovirus cargo is high, it’s generally not as long lasting as AAV (up to several weeks vs. AAV’s several years...-associated Virus (AAV) Guide Addgene’s viral plasmids and resources Addgene’s viral services: AAV ...

  3. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    Published
    April 13, 2023, 1:15 p.m.
    ...sequence for degradation. Viral introduction of shRNA plasmids can induce gene-specific silencing temporarily...transfection and random genomic integration of a plasmid. Viral transduction is also generally less toxic...response 2) very high immunogenicity 3) and long-lasting immune response even after a single dose. ...their use in other clinical applications. In the last decade, viral applications in cancer treatment and...

  4. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    Published
    April 10, 2018, 12:56 p.m.
    ... a reporter and a nanobody that’s encoded on a plasmid. A nanobody is a ~150 amino acid, single domain... your own RANbodies? Great! All of the RANbody plasmids from Yamagata et al can be found here. Can’t find...consider making you own DNA ladders with these plasmids from Penn State Need help picking the right fluorescence...Resources on Addgene.org More nanobody expression plasmids can be found here Watch this video to learn more...

  5. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    Published
    Jan. 16, 2018, 3:20 p.m.
    ...Fluorescent protein plasmids Use FRET to study protein-protein interactions Deposit plasmids with Addgene! ...visualizing protein turnover in situ in neurons. The classic approach for studying protein turnover is radioactive...Tagged protein can easily be overexpressed from a plasmid but overexpression may interfere with the system...

  6. Human Germline Editing Using CRISPR

    Type
    Blog Post
    Published
    Aug. 10, 2017, 2:19 p.m.
    ...skin, blood and semen samples. Ma et al. designed plasmids containing Cas9 and two different gRNAs, and electroporated... studies, the edited embryos proceeded to the blastocyst stage at similar rates to untreated controls....researchers were able to examine each individual blastomere to determine its genotype. However, this detailed...CRISPR Resources on Addgene.org Browse All CRISPR Plasmids Check Out Our CRISPR Guide Pages ...

  7. Antibodies 101: Conjugation

    Type
    Blog Post
    Published
    March 26, 2024, 1:15 p.m.
    ...Enzymes An equally, if not more, common conjugate class than fluorophores are enzymes. The most common enzyme...clinical applications). But there are a few other classes that we would be remiss not to at least mention...ConjugatesAntibodies 101: The Basics of Western BlottingPlasmids 101: Biotinylation Resources on Addgene.org...Addgene.org Unconjugated antibodies at AddgeneAntibody plasmids at Addgene...

  8. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 3, 2022, 1:15 p.m.
    ...popular split-FP plasmids from our catalog to help you find the right split-FP plasmid for your experiment...below, and click the Article link to find all the plasmids from the associated paper:   Color Base FP...and experimental design. We hope you find these plasmids useful. Good luck, and happy experimenting! References...

  9. How to Prepare for an Industry Interview

    Type
    Blog Post
    Published
    July 9, 2020, 1:15 p.m.
    ...starting with the hiring manager. These interviews lasted for 30 minute to an hour. Although I never experienced...prior research project. In total, the interviews lasted from half to all of the day.  This whole process...technical difficulties. Have your presentation on a flash drive and consider bringing your own laptop as a... is complete Industry interviews are long! Mine lasted 4.5 hours, which was a lot of extraversion for ...

  10. CRISPR in the Clinic

    Type
    Blog Post
    Published
    Nov. 2, 2023, 1:15 p.m.
    ...underway to explore CRISPR’s capacity for both classes of edits in cancer therapy. Blood disorders Two...Disease (SCD) and Transfusion-dependent beta thalassemia (TBT), both of which affect hemoglobin. Most ...introducing a desired edit, but this particular class of treatments has quite the opposite aim: to destroy...ijms24021563 Resources on Addgene.org CRISPR Plasmids and Resources CRISPR 101 eBook CRISPR History ...

  11. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    Published
    March 7, 2024, 2:39 p.m.
    ...Want to get your hands on SpRY? Find a mammalian plasmid here and edit on! References and Resources References..., 6175 (2023). DOI:10.1038/s41467-023-41829-y (Plasmids available here) Walton, R. T., et al., Unconstrained...368,290-296(2020). DOI:10.1126/science.aba8853 (Plasmids available here)   Nishimasu, H., et al.,Engineered... on Addgene.org Addgene’s CRISPR Guide CRISPR Plasmids and Resources Resources on the Addgene blog ...

  12. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    Published
    March 15, 2016, 2:30 p.m.
    ...for expression of a gene carried on the transfer plasmid or quantifying the number of viral copies that ...beneficial as they ensure that the correct transfer plasmid was used in the transfection; if preparing several... that comes from amplification of the original plasmid which can over-inflate results. A packing vector...antibiotic resistance genes carried on transfer plasmids. Target cells are transduced with serial dilutions...

  13. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ...Agarose Method Plasmids 101: Mammalian Vectors Resources on Addgene.org Find mammalian plasmids for your ... It’s good practice to label your plates and/or flasks with your name, date, the cell line you’re using...records of your experiments. Autoclave is the rave! Last, but not least, any lab material that is intended...

  14. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ...resources on addgene.org Find Optogenetics Plasmids Find Biosensor Plasmids Visit the Fluorescent Protein Guide...Frank Hayward et al., 2024). They deposited their plasmid with Addgene, hoping to inspire others to explore...Lab is eager to hear from you. Find the MagLOV plasmid here!   References and resources References Duret...

  15. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    Published
    May 4, 2017, 2:30 p.m.
    ...them in ways that make them much easier. A special class of FPs, the FP timers, add an entire new dimension....  Table 1: Monomeric FP Timers and Associated Plasmids from Subach et al. Protein Excitation (nm) ...) Emission (nm) Brightness pKa Maturation Plasmids Fast-FT 403 (blue), 583 (red) 466 (blue), 606 (red...dynamics of vesicle fusion and release from the plasma membrane by fusing various vesicle cargoes to FP...

  16. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type
    Blog Post
    Published
    Aug. 3, 2023, 1:15 p.m.
    ...tool for those wishing to run larger panels: five laser spectral flow machines have 64 detectors, allowing...spectra for two fluorophores excited by the violet laser, BV421 and BV711. These “rainbow box” plots are ...full emission spectra for BV421 and BV711 on a 3 laser spectral flow cytometer   Using spectral flow...Similarity index across 12 different colors on a three laser spectral flow cytometer.   Level of co-expression...

  17. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ...Antibodies 101: Monoclonal Antibodies Plasmids 101: Peptide Tagging Plasmid-based Recombinant Antibody Resources.... In two studies investigating mechanisms of endoplasmic reticulum localization, Munro and Pelham casually... recombinant anti-c-Myc [9E10] at Addgene Find plasmids with the myc-tag  ...

  18. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    Published
    Oct. 17, 2017, 12:55 p.m.
    ...the complex is docked on the membrane of the endoplasmic reticulum. And graduate students, momentarily...a surface format made of rock-hard, inflexible plaster. As a result, the model obscures the flexible nature...Designs web site. Or, if you are visiting a local classroom or trying to explain your science to the general...Guide Page CRISPR/Cas9 History Browse All CRISPR Plasmids and Resources ...

  19. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    Published
    July 9, 2019, 12:52 p.m.
    ...school system that has made strong efforts in the last 5 years to incorporate more science and technology...page in terms of the vocabulary--DNA, protein, plasmid, gene, etc. Though I talked about CRISPR’s molecular...should we use CRISPR? How shouldn’t we use it? This last section was arguably the most important, and it ...out our comprehensive CRISPR guide Find CRISPR plasmids and resources ...

  20. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    ...vectorization strategies include creating an AdV transfer plasmid with the E1 and E3 (Early 1 and Early 3) genes .... These genes’ functions are rescued by helper plasmids used during production and/or by genes present...made available by this deletion from the genomic plasmid can be used to house a recombinant sequence (transgene...late genes are usually present in the transfer plasmid for the majority of adenoviral vector systems used...
Showing: 501 - 520 of 809 results