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We narrowed to 729 results for: NSI;

Showing: 501 - 520 of 729 results

  1. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ... capture large field-of-views (6). For more comprehensive reviews on several other LSFM configurations...Bradke laboratories later developed the 3DISCO (3-dimensional imaging of solvent cleared organs) method of ...

  2. Tips for arabidopsis transformation

    Type
    Blog Post
    Published
    Oct. 25, 2018, 1:23 p.m.
    ...that is wide enough to dunk your plant’s flowers inside. 50 mL falcon tubes or plastic weigh boats are ...from a colony on a plate will reach an optimal density in 18-24 hours. I then add 0.5 mL of this starter...

  4. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ...enzymes do not contain the RuvC and HNH domains responsible for DNA cleavage, so they cannot directly edit.... PspCas13b does not require a PFS, and it is sensitive to mismatches in target RNA from bases 12-26 of...

  5. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    Published
    July 30, 2020, 1:15 p.m.
    ...biologist--as an example of “big science,” a massive, expensive project that required collaboration from scientists...five Addgene colleagues who read it with me, the consistent response from scientist and non-scientist alike...

  6. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    Published
    Jan. 16, 2020, 2:18 p.m.
    ...For these reasons, we are in the process of transitioning our titering method from qPCR to ddPCR. Droplet...titer your AAV by ddPCR. Droplet digital PCR is a sensitive assay, so cross contamination into a no template...

  8. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    Published
    Feb. 9, 2023, 2:15 p.m.
    ...students. But he also found that his teaching responsibilities left him with limited time for one-on-one ...notes the importance of acknowledging student apprehensions about “failure” (protocols not working, hypotheses...

  9. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    Published
    Nov. 29, 2022, 2:15 p.m.
    ...connections between biology and ancestral groups or, by extension, race. Many researchers do large-scale CRISPR ...potential for bias but to actively look for and consider bias as a confounding factor when interpreting...

  10. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    Published
    Oct. 4, 2018, 12:44 p.m.
    ...human CRISPRko sgRNA library The Brunello library consists of 77,441 sgRNAs with an average of 4 sgRNAs per...Mediator) uses an improved activator design that consists of inactive Cas9-VP64, a sgRNA with two MS2 RNA...

  11. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    Published
    May 19, 2016, 2:30 p.m.
    ...non-dividing cells. The first lentiviral vector system consisted of three plasmids: the packaging, envelope, and...capacity of a lentiviral vector but for which transient transgene expression is sufficient. Lentiviral...

  12. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    Published
    Oct. 22, 2015, 2:30 p.m.
    ...Saccharomyces cerevisiae in 1989 and are still used extensively to screen for molecular interactions in the cell...post-translationally modified, co-expression of the enzyme responsible for the modification in the assay host strain...

  13. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    Published
    Sept. 3, 2015, 2:30 p.m.
    ... Although we are busy with many other work responsibilities, we make team building activities a priority...at Addgene. In addition to her operational responsibilities, she enjoys organizing team building events...

  15. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    Published
    May 8, 2018, 1 p.m.
    ...editing field by making experimental workflows considerably easier, faster, and more efficient than previous...novice to expert-level researchers in achieving consistently successful CRISPR experiments. Jessica is a ...

  16. Transferable Skills Guide: Creativity

    Type
    Blog Post
    Published
    Jan. 18, 2022, 2:15 p.m.
    ...with idea generation, try this thought exercise: Consider the issue as if there were no restraints on your...something completely unrelated to the issue and consider how it could be shaped to solve your problem. ...

  17. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    Published
    July 30, 2024, 1:15 p.m.
    ...our own repository data, giving us significant insight into which vectors are likely to be of most use...Swanson, L. E., & Fan, M. (2016). Practical Considerations for Using Pooled Lentiviral CRISPR Libraries...

  18. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    Published
    June 20, 2024, 1:15 p.m.
    ...plasmids tend to be high copy number. Here, we will consider the replication mechanism of plasmids under stringent...plasmid to balance the total plasmid number.  Let’s consider pSC101. This plasmid is stringently controlled...

  19. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    Published
    Dec. 13, 2016, 3:30 p.m.
    ...from many other types of cancer. Mesothelioma is considered an orphan cancer; it is rare disease with only...non-resectable mesothelioma by the National Comprehensive Cancer Network, a coalition of the top 27 cancer...

  20. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ...CRISPR/Cas Toolkit for plants includes 95 plasmids consisting of CRISPR/Cas nucleases, base editors, gRNA backbones...activation-based norepinephrine (GRABNE) sensors allow for sensitive and high spatiotemporal measurements of norepinephrine...
Showing: 501 - 520 of 729 results