We narrowed to 629 results for: Ncl

1. Antibodies 101: Flow Cytometry Controls

   Type

   Blog Post

   Published

   June 6, 2024, 1:50 p.m.

   ... the antibodies you are using, you may need to include additional controls like FMO or isotype controls...

2. Viral Vectors 101: Viral Vector Elements

   Type

   Blog Post

   Published

   July 25, 2023, 1 p.m.

   ... transcription of other relevant viral genes (including the ones listed previously). E3/E1B suppresses...

3. Viral Vectors 101: Viruses as Biological Tools

   Type

   Blog Post

   Published

   March 21, 2023, 1:15 p.m.

   ...been approved in the US for multiple diseases, including hemophilia B and spinal muscular atrophy.   ...

4. Sharing is Caring: But How to Distribute Open Hardware?

   Type

   Blog Post

   Published

   Feb. 28, 2023, 2:15 p.m.

   ...panel shows workflow of open hardware.    Conclusion Addgene is a great example of how sharing of ...

5. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

   Type

   Blog Post

   Published

   July 28, 2017, 1:30 p.m.

   ...is most often used, but other selection markers include vermillion (another gene controlling eye color)...

6. A Practical Approach to Choosing the B(right)est Fluorescent Protein

   Type

   Blog Post

   Published

   June 1, 2017, 2:30 p.m.

   ...application specific parameters into account, including the specs of your microscope (excitation wavelength...

7. Screening for Successful Genome Editing with Digital PCR

   Type

   Blog Post

   Published

   March 30, 2017, 2:30 p.m.

   ...will focus on assays to detect NHEJ. These assays include a forward and reverse primer to amplify the target...

8. Hot Plasmids - May 2022

   Type

   Blog Post

   Published

   May 26, 2022, 1:15 p.m.

   ... remains a challenge due to multiple factors, including limitations of number of flourescent proteins ...

9. Antibodies 101: ChIP

   Type

   Blog Post

   Published

   March 10, 2022, 3:25 p.m.

   ...into DNA expression and protein interactions, including epigenetic modifications. But it's not an approach...

10. Sweating the Small Stuff: Details in the Lab

    Type

    Blog Post

    Published

    Feb. 8, 2022, 2:15 p.m.

    ... of making an error. Try a variety of things, including (and maybe especially) suggestions you are initially...

11. Antibodies 101: Buffers, Storage, and Conjugates

    Type

    Blog Post

    Published

    Feb. 1, 2022, 2:15 p.m.

    ...commercial antibodies come with sodium azide already included in the buffer, which will be noted on the product...

12. GPCRs: How Do They Work and How Do We Study Them?

    Type

    Blog Post

    Published

    Feb. 11, 2021, 2:15 p.m.

    ...be activated by an array of different ligands, including hormones, neurotransmitters, photons or odorants...

13. 7 Tips to Secure a STEAM Internship This Summer

    Type

    Blog Post

    Published

    Feb. 2, 2021, 2:15 p.m.

    ...and cover letter revised by your network, which includes your friends, family members, and professional...

14. New and Upcoming Viral Vectors - May 2020

    Type

    Blog Post

    Published

    May 28, 2020, 1:15 p.m.

    ...neurons. We have expanded our AAV collection to include two new ChR2 constructs that allow for targeted...

15. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

    Type

    Blog Post

    Published

    May 19, 2020, 1:15 p.m.

    ...StrepII, 3xFLAG, 6xHIS, MBP, Myc). These constructs include proteins like nonstructural proteins 1 to 16, spike...

16. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

    Type

    Blog Post

    Published

    March 17, 2020, 1:32 p.m.

    ...and Cas12 homologs that the researchers tested, including SpCas9, SaCas9, LbCas12a, enAsCas12a, SpCas9-NG...

17. Early Career Researcher Toolbox: Social Media for Scientists

    Type

    Blog Post

    Published

    Dec. 11, 2018, 12:46 p.m.

    ...And the blog writing tips are elements I try to include in each post I write for the Addgene blog. 5. "...

18. How Addgene Creates Content

    Type

    Blog Post

    Published

    Nov. 17, 2022, 2:15 p.m.

    ...team, we identified what applications should be included and what basic information someone new to antibodies...

19. Plasmids 101: Screens vs. Selections

    Type

    Blog Post

    Published

    Nov. 10, 2022, 2:15 p.m.

    ...Things to consider when selecting an assay system include: How specific is it? Is it high throughput? Will...

20. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type

    Blog Post

    Published

    June 2, 2015, 6:06 p.m.

    ...described in this post in the graphic below - including some simple comparisons. The easy multiplexing...