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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 751 results for: Nes;

Showing: 501 - 520 of 751 results

  1. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    Published
    June 15, 2023, 1:15 p.m.
    ...focus on development, along with the inherent trickiness of using and optimizing GCE, led to a large toolbox...essentially those needed to make site-directed mutagenesis, many researchers new to GCE aren’t able to successfully...

  2. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    Published
    Feb. 28, 2023, 2:15 p.m.
    ...microscope has been a powerful tool in almost all disciplines. In biology, innovative new imaging approaches...shows an image of differentiating monocyte with planes and hardware to it. Right panel shows workflow ...

  3. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    Published
    May 28, 2020, 1:15 p.m.
    ...inhibition library that inhibits over 18,000 human genes and is used for genome-wide inhibition screening... of the extensive supply of transgenic reporter lines that supply a variety of GFP expression patterns...

  5. Early Career Researcher Toolbox: Social Media for Scientists

    Type
    Blog Post
    Published
    Dec. 11, 2018, 12:46 p.m.
    ...interviews on Al Jazeera and NPR, articles in magazines like Fast Company, opportunities to speak at conferences...be wondering how to get started. This blog post outlines 10 steps to take when starting to use Twitter ...

  7. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    Published
    March 23, 2017, 2:30 p.m.
    ...involved every Addgenie in the company. Our Finance, Business and Legal teams had to pave the way for this project...support for export and shipping on dry ice. The Business and Finance Teams continue to facilitate the formation...

  8. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    Published
    April 16, 2015, 3:45 p.m.
    ...Ramsden DA (2014) The fidelity of the ligation step determines how ends are resolved during nonhomologous end...Protocol for Genomic Deletions in Mammalian Cell Lines [Video] Pooled CRISPR Libraries Offer Genome-Wide...

  9. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...we wanted to walk through some of our favorite milestones and share some thoughts from the community that... From day one, we built systems for now-common business practices, like accepting e-signatures and designing...

  10. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    Published
    April 20, 2023, 4 p.m.
    ...silence–but it was thorough and coherent.  To be honest, if my paper hadn’t been ridiculously more complex... in front of a very confused group of visiting Chinese undergrads. You get nervous and freeze, or forget...

  11. To Each HIS Own

    Type
    Blog Post
    Published
    March 2, 2023, 2:15 p.m.
    ...acid, proteins containing two or more adjacent histidines are present in most production systems and can...can affect protein function. B, The string of histidines in the tag is typically between 6 to 9 residues...

  12. Summer SciComm: Preprints

    Type
    Blog Post
    Published
    Aug. 16, 2022, 1:15 p.m.
    ...transparency Another major benefit of preprints is their openness. Preprints can be read freely by anyone and are... work too openly too soon or simply a lack of awareness of the benefits of preprints. Some worry about...

  13. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    Published
    March 15, 2022, 1:15 p.m.
    ...you cannot test them all, so how do you know which ones to try?  Read on and we will provide you with some...in knock-out or knock-down down tissues and cell lines; orthogonal strategies that compare antibody assay...

  14. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    Published
    Dec. 21, 2021, 2:15 p.m.
    ...methods are often significantly longer than direct ones.  Even though the secondary antibody incubation ...to improve direct methods and avoid the indirect ones. Some Western blots, for example, use a primary ...

  16. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    Published
    April 10, 2018, 12:56 p.m.
    ...complete. The nanobody based RANbody platform from the Sanes Lab overcomes this limitation and allows for the...its sequence in the comments below. References Sanes, J.R., & Yamagata, M. (2018). Reporter-nanobody ...

  17. Human Germline Editing Using CRISPR

    Type
    Blog Post
    Published
    Aug. 10, 2017, 2:19 p.m.
    ...but this advantage likely won’t transfer to all genes we’d like to target. In addition, as on-target editing...about High Fidelity CRISPR Variants Target Multiple Genes at Once with CRISPR Resources on Addgene.org Browse...

  18. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type
    Blog Post
    Published
    June 15, 2017, 2:30 p.m.
    ...essential to verify a number of key properties (brightness, photostability, oligomerization) of a number...Additional Resources on Addgene.org Find Empty Backbones with Fluorescent Tags Find Plasmids for Subcellular...

  19. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    Published
    April 13, 2017, 2:30 p.m.
    ...GFP-tagged Atg8p or LC3: ATG8 is part of a group of genes that affect autophagy (ATG) in yeast. Atg8p (“p”...stands for protein) associates with autophagosomal membranes, so tagging it with GFP allows for tracking the...

  20. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    Published
    Nov. 21, 2019, 1:53 p.m.
    ...words —> decreasing frustration —> increasing the happiness. My unofficial motto in my Product Manager role...effective approaches for clearing up communications lines in your professional life, let me know that, too...
Showing: 501 - 520 of 751 results