We narrowed to 552 results for: PPL;

1. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

   Type

   Blog Post

   Published

   Oct. 4, 2018, 12:44 p.m.

   ...Calabrese pooled library and lentiviral prep here! Applications for the Root and Doench CRISPR libraries Overall...

2. AAVs in Retinal Gene Therapy

   Type

   Blog Post

   Published

   July 31, 2018, 12:55 p.m.

   ...-1250015778 7. Yu W and Wu Z., 2018. In vivo Applications of CRISPR-Based Genome Editing in the Retina...

3. Plasmids 101: Secondary Nanobody Toolbox

   Type

   Blog Post

   Published

   Feb. 27, 2018, 2:04 p.m.

   ..., Alexa Fluor 568, or Alexa Fluor 647 before application to the sample. Staining with this multi-color...

4. Better Dyeing Through Chemistry & Small Molecule Fluorophores

   Type

   Blog Post

   Published

   Sept. 8, 2016, 2:30 p.m.

   ...labels for fixed-cell imaging, not for live-cell applications. Thus, these fluorophores are often bulky and...

5. Tips for Screening with Yeast Two Hybrid Systems

   Type

   Blog Post

   Published

   Oct. 22, 2015, 2:30 p.m.

   ...or growth stage). A prey library can either be applied to the bait in a single population (e.g. transforming...

6. Plasmids 101: Protein tags

   Type

   Blog Post

   Published

   Dec. 11, 2014, 4:26 p.m.

   ...deciding on which one to use will depend on your application (see table below). The antibodies available for...

7. Save Time with Transient Plant Leaf Transformations

   Type

   Blog Post

   Published

   July 25, 2019, 12:03 p.m.

   ...placed in an Agrobacterium solution and a vacuum is applied with a vacuum bell or desiccator. Successful infiltration...

8. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

   Type

   Blog Post

   Published

   July 23, 2019, 12:46 p.m.

   ... interested in using AAV-PHP.B vectors? See Supplementary Table 3 in Huang et al. for a list of PHP.B ...

9. Twenty Years of Sharing: Addgene's Viral Vector Service

   Type

   Blog Post

   Published

   July 30, 2024, 1:15 p.m.

   ...viral vector service in 2016. We realized we could apply the lessons learned from our plasmid lab to develop...

10. To Codon Optimize or Not: That is the Question

    Type

    Blog Post

    Published

    Nov. 12, 2020, 2:15 p.m.

    ...synonymous codon usage bias, and its potential applications. Nucl Acids Res 15:1281–1295 . https://doi.org...

11. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type

    Blog Post

    Published

    Aug. 20, 2020, 2:30 p.m.

    ...to dramatically increase the number of CRISPR applications. The first non-Cas9 CRISPR protein adapted for...

12. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type

    Blog Post

    Published

    Oct. 13, 2022, 1:45 p.m.

    ...Motif (PAM), the sequence NGG.  If these conditions apply to your experimental target, SpCas9 and its enhanced...

13. Viral Vectors 101: Pseudotyping

    Type

    Blog Post

    Published

    April 20, 2021, 2:30 p.m.

    ...Wickersham et al., 2007). This system requires first supplying rabies glycoprotein along with the TVA receptor...

14. "Hall of Fame" AAV Enhancers from the Allen Institute for Brain Science

    Type

    Blog Post

    Published

    Sept. 9, 2025, 1:15 p.m.

    ...when discussing raising children, it can also be applied to research. Sharing knowledge and tools is the...

15. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

    Type

    Blog Post

    Published

    July 8, 2025, 1:15 p.m.

    ...Modular Cloning Plasmids 101: Modular Cloning Applications and Kits Plasmids 101: Golden Gate Cloning ...

16. Plasmids 101: Shuttle Vectors

    Type

    Blog Post

    Published

    May 20, 2025, 1:15 p.m.

    ...vector, the deficient cells are grown in media that supplies the key nutrient. The vector provides the missing...

17. CRISPR 101: Any Base Transversion Editors

    Type

    Blog Post

    Published

    April 1, 2025, 1:15 p.m.

    ...researchers can now select the best tool for their application from among many options.  Conclusion With these...

18. CRISPR-mediated Plant Base Editors

    Type

    Blog Post

    Published

    Jan. 3, 2019, 1:35 p.m.

    ...question probably comes to mind: can CRISPR be applied to fix the defective base precisely? Until 2016...

19. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

    Type

    Blog Post

    Published

    Sept. 13, 2018, 1:55 p.m.

    ...noise, of dsDNA breaks caused by CRISPR. See supplemental figure 6 for more details. Safe-targeting guides...

20. Mesothelioma - Causes, Symptoms, and Treatment

    Type

    Blog Post

    Published

    Dec. 13, 2016, 3:30 p.m.

    ...pemetrexed and cisplatin –because it cuts off the blood supply to fast-growing cancer tumors. In fact, the use...