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We narrowed to 837 results for: RING

Showing: 501 - 520 of 837 results

  1. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    Published
    Jan. 9, 2020, 1:45 p.m.
    ...publications in the fields of research they support. Monitoring these types of accounts can help you find papers...papers later. Pro tip Learn more about authors by exploring author pages (for example, Melina Fan’s author... Instead, you create your own research feed by entering keywords, concepts, journals, and/or papers of...

  2. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

    Type
    Blog Post
    Published
    Aug. 20, 2019, 12:31 p.m.
    ...faster in the following test and skew results measuring locomotion. Mice may need to be given some time...optional handling animals Experiment Behavioral scoring systems (experimenter differences in behavior observation...of our viral vectors blog posts Find tips for preparing AAVs View our Beginner's Guide to Viral Vectors...

  3. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    Published
    Sept. 27, 2017, 1:23 p.m.
    ...create a successful science blog  5) Get creative – Bring your knacks into play Perhaps writing just isn’t...projects, you can set up a citizen science project. Answering the big science questions around climate change...guest blogger, Sarah Schmidt! Sarah Schmidt is engineering resistance against Fusarium wilt disease in bananas...

  4. Engaging with science and society at pgEd

    Type
    Blog Post
    Published
    Sept. 20, 2018, 12:17 p.m.
    ...disparities. But as the science moves forward at a blistering pace, it is becoming ever more urgent for scientists...ways of collaborating, and to organize events that bring different voices together to talk about genetics...running these events will be important both in preparing you for future work in these areas, and in helping...

  5. Educational Planarian Tools for Teaching Developmental Biology Techniques

    Type
    Blog Post
    Published
    May 24, 2018, 1:20 p.m.
    ...characterized in an unrelated worm (C. elegans) [2]. Delivering dsRNA matching a specific mRNA sequence triggers...and silences its expression. More specifically, during the process of RNAi, an enzyme dubbed Dicer cuts...smaller dsRNA fragments are referred to as small interfering RNAs (siRNA) and they bind to a class of Argonaute...

  6. Lab Automation at Addgene

    Type
    Blog Post
    Published
    March 22, 2018, 1:28 p.m.
    ...out your samples. This can be useful if you’re transferring samples from 96 wells plates onto agar q-trays...bacterial samples at the same time as opposed to preparing each sample individually. Enter the BIOROBOT! ...brushes the information onto the vials instead of requiring us to put a label on each tube by hand. We’ll ...

  7. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 3, 2022, 1:15 p.m.
    ...produced when the candidate proteins’ interaction brings the pieces of the FP back together (Figure 1). ...interaction in vivo or in vitro, and can be used for measuring protein expression or localization in cells, or...yeast TREX-2 mRNA export complex: components and anchoring nucleoporin. Lu et al. Plant J. 2010 Yuhai Cui...

  8. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type
    Blog Post
    Published
    Dec. 14, 2021, 2:15 p.m.
    ...University. Some enzymatic fusions are godsends. Tethering dCas9 to the histone acetyltransferase p300 or... 2018). This means that off-target loci – in neighboring chromatin or throughout the genome – could be...that site. So, when DNMT1 methylates the genome during DNA replication, it doesn’t methylate sites blocked...

  9. Design Tips for Prime Editing

    Type
    Blog Post
    Published
    Jan. 23, 2025, 2:15 p.m.
    ...extension begins with a C base can cause unwanted base pairing (left), while pegRNAs that use other bases in this...the cell to use the edited strand as a template during DNA repair. Test multiple nick sites, starting ... linkers that minimize unwanted intra-RNA base pairing with the primer binding site (Nelson et al., 2022...

  10. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ...here!   Zhang, Z., et al. (2023). Efficient engineering of human and mouse primary cells using peptide-assisted...effective at breaching the blood-brain barrier and delivering a transgene, with neuron bias observed in young...the Gradinaru lab, AAV1-X1, was developed by transferring the 7-mer peptide region from variable region...

  11. 6 Tips for Grant Writing

    Type
    Blog Post
    Published
    Oct. 18, 2016, 2:30 p.m.
    ...approach that problem in a novel way. Finally, engineering new applications to solve problems will provide...’s a good idea to move around to different labs during your career; you learn new techniques and interact...get feedback from the funding body through their scoring system or meeting with their grant submission panel...

  12. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    Published
    Oct. 22, 2014, 1:57 p.m.
    ...the moderator should be taking good notes and discovering interesting stories, riffs or opinions that each... a fun discussion. People are much better at remembering these things on a relaxed phone call than when...The idea is to ask questions in a directed way to bring out these interesting stories and remind the panelists...

  13. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    Published
    Feb. 4, 2020, 2:15 p.m.
    ...mass bleaching, the loss of their symbiotic algae, during heat stress. For other organisms, like the sea ... Wistrand-Yuen, P., … Forster, A. C. (2018). Engineering a palette of eukaryotic chromoproteins for bacterial...bacterial synthetic biology. Journal of Biological Engineering, 12(1). https://doi.org/10.1186/s13036-018-0100...

  14. Academic vs. Industry Postdocs

    Type
    Blog Post
    Published
    Nov. 13, 2018, 1:22 p.m.
    ...an industry postdoc is something that you’re considering. Like anything, there are pros and cons to postdocs...number of steps, decisions and people involved is staggering. The process is complicated. If you’re interested...all about teamwork. A single individual could not bring a drug to the market, so people in industry have...

  15. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

    Type
    Blog Post
    Published
    July 13, 2017, 2:30 p.m.
    ... unable to participate in genetic recombination during meiosis. Also, flies are not viable if they are...know is that males do not recombine chromosomes during meiosis. This can sometimes be a handy feature ...Like Addgene, the Drosophila community is highly sharing-oriented, and there are many resources to help ...

  16. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    Published
    May 4, 2017, 2:30 p.m.
    ...separate proteins should be useful for monitoring cellular events occurring at varied time scales.  Table 1:...the FP timers, add an entire new dimension to monitoring localization; using FP timers, researchers can...

  17. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    Published
    Feb. 11, 2025, 2:15 p.m.
    ...more gRNAs must be used along the same chromosome. During DNA repair, there is a chance that the two breakpoints...Harnessing type I CRISPR–Cas systems for genome engineering in human cells. Nature Biotechnology, 37(12),...CRISPR-Cas Systems INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons Additional resources...

  18. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ...powerful tool, it was promising enough to keep exploring and to try developing a variant with an enhanced...variants of MagLOV will be possible with other engineering approaches and that magnetosensitive tools can...often posting updates on social media) and freely sharing code, raw data, and more. The team shares results...

  19. Pooled Library Amplifications

    Type
    Blog Post
    Published
    May 30, 2024, 1:30 p.m.
    ... library is maintained and reduce recombination during the amplification process. In liquid culture, all...recombination. If you do see recombination, we recommend ensuring the cells used are meant for repetitive sequences...Addgene blog Genome-Wide Screening Using CRISPR Preparing Pooled Libraries NGS Quality Control for Pooled...

  20. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    Published
    May 26, 2016, 2:30 p.m.
    ...media with supplements such as magnesium salts, buffering agents, and/or supplying additional carbon sources... yield. Start with a single, fresh colony Sub-culturing directly from a frozen glycerol stock or agar ...harvesting and miniprepping at various time points. Measuring the optical density of the culture can indicate...
Showing: 501 - 520 of 837 results