We narrowed to 526 results for: She

...resulting in off-target effects. A technique that pushes the size limitations of ssODNs is Easi-CRISPR (...

...case, however, and recent work has already been published on improving base editing precision. Similar treatments...

... Alyssa Shepard...

...want, but that means the other party will feel punished or invalidated. Both compromise and arbitration...

...reliable in the future.  We hope that this post has shed some light on immunofluorescence. The technique ...

...dermis and identify that Langerhans cells are replenished through the proliferation of specific, and not...

...1731-1745. PubMed PMID: 26960991. 9. Chatterjee, Abhishek, et al. "A genetically encoded fluorescent probe...

... GeneArt® Seamless Cloning and Assembly (Thermo Fisher Scientific) NEBuilder® HiFi DNA Assembly (NEB) ...

...the secondary antibody, it should get completely washed away and leave no signal. If you see lots of extra...

...Originally published Feb 14, 2017 and updated Jun 24, 2020. Epigenetic modifications are an additional...

...PAM (bold highlighted text) and cleavage sites (slashes) are shown. In this case, the green guide would...

...world, I (Wenning) was fascinated by CRISPR and rushed to test it. I still remember the day, March 16,...

...ratio” in the ligation reaction. 5. Purify the finished plasmid   Once it looks like your ligation has...

...837597 Li J-F, Chung HS, Niu Y, Bush J, McCormack M, Sheen J (2013) Comprehensive Protein-Based Artificial ...

...New England Biolabs® (NEB) researchers recently published a method for the high-throughput profiling of ...

... generations of lentiviral vectors have been established so far, with safety increasing in each generation...

...out of the solution. Once the unbound protein is washed away, the protein of interest is dissociated from...

... Alyssa Shepard...

...first resuspended in a pressurized buffer called sheath fluid and transported through tubes or capillaries...

... tagging is scalable to 96-well format. Off-the-shelf CRISPR reagents for the knock-in experiment were...