We narrowed to 526 results for: She

1. CRISPR 101: Homology Directed Repair

   Type

   Blog Post

   Published

   Jan. 26, 2023, 2:15 p.m.

   ...resulting in off-target effects. A technique that pushes the size limitations of ssODNs is Easi-CRISPR (...

2. Transferable Skills Guide: Conflict Resolution

   Type

   Blog Post

   Published

   Jan. 4, 2022, 2:15 p.m.

   ...want, but that means the other party will feel punished or invalidated. Both compromise and arbitration...

3. Antibodies 101: Introduction to Immunofluorescence

   Type

   Blog Post

   Published

   Nov. 16, 2021, 2:15 p.m.

   ...reliable in the future.  We hope that this post has shed some light on immunofluorescence. The technique ...

4. Five Popular Model Organisms, Part 2

   Type

   Blog Post

   Published

   Sept. 29, 2020, 1:15 p.m.

   ...with their regenerative abilities is expected to shed light on human aging pathological developments (...

5. The Advances Behind The World's First Personalized CRISPR Treatment

   Type

   Blog Post

   Published

   Sept. 16, 2025, 1:15 p.m.

   ...case, however, and recent work has already been published on improving base editing precision. Similar treatments...

6. Plasmids 101: Expressing shRNAs for RNA Interference

   Type

   Blog Post

   Published

   June 17, 2025, 1:15 p.m.

   ... Alyssa Shepard...

7. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

   Type

   Blog Post

   Published

   Dec. 2, 2019, 2 p.m.

   ...molecules per cell volume? A call to rethink some published values." Bioessays 35.12 (2013): 1050-1055. PubMed...

8. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

   Type

   Blog Post

   Published

   March 1, 2016, 3:30 p.m.

   ... GeneArt® Seamless Cloning and Assembly (Thermo Fisher Scientific) NEBuilder® HiFi DNA Assembly (NEB) ...

9. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

   Type

   Blog Post

   Published

   April 5, 2018, 12:42 p.m.

   ...PAM (bold highlighted text) and cleavage sites (slashes) are shown. In this case, the green guide would...

10. Antibodies 101: Multiplex Immunofluorescence

    Type

    Blog Post

    Published

    May 23, 2023, 1:15 p.m.

    ...the secondary antibody, it should get completely washed away and leave no signal. If you see lots of extra...

11. CRISPR 101: Epigenetics and Editing the Epigenome

    Type

    Blog Post

    Published

    June 24, 2020, 5:45 p.m.

    ...Originally published Feb 14, 2017 and updated Jun 24, 2020. Epigenetic modifications are an additional...

12. Generating Mouse Models Using CRISPR/Cas9

    Type

    Blog Post

    Published

    July 12, 2016, 2:30 p.m.

    ...world, I (Wenning) was fascinated by CRISPR and rushed to test it. I still remember the day, March 16,...

13. Plasmids 101: Restriction Cloning

    Type

    Blog Post

    Published

    Feb. 18, 2016, 3:42 p.m.

    ...ratio” in the ligation reaction. 5. Purify the finished plasmid   Once it looks like your ligation has...

14. When Fidelity Matters: A frank discussion about ligase fidelity

    Type

    Blog Post

    Published

    March 6, 2018, 1:49 p.m.

    ...New England Biolabs® (NEB) researchers recently published a method for the high-throughput profiling of ...

15. Quick Guide to All Things Lentivirus

    Type

    Blog Post

    Published

    March 21, 2017, 2:30 p.m.

    ... generations of lentiviral vectors have been established so far, with safety increasing in each generation...

16. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type

    Blog Post

    Published

    Oct. 27, 2020, 1:15 p.m.

    ...837597 Li J-F, Chung HS, Niu Y, Bush J, McCormack M, Sheen J (2013) Comprehensive Protein-Based Artificial ...

17. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type

    Blog Post

    Published

    June 28, 2018, 3:01 p.m.

    ... tagging is scalable to 96-well format. Off-the-shelf CRISPR reagents for the knock-in experiment were...

18. Custom CRISPR Screens & the Green Listed Software

    Type

    Blog Post

    Published

    July 11, 2017, 2:30 p.m.

    ...like to use in your screen. Currently, eleven published libraries are embedded. These were kindly donated...

19. Typing CRISPR Systems

    Type

    Blog Post

    Published

    March 18, 2025, 1:15 p.m.

    ... Alyssa Shepard...

20. Antibodies 101: Flow Cytometry

    Type

    Blog Post

    Published

    July 20, 2021, 1:15 p.m.

    ...first resuspended in a pressurized buffer called sheath fluid and transported through tubes or capillaries...