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We narrowed to 867 results for: TOR

Showing: 501 - 520 of 867 results

  1. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    Published
    June 2, 2015, 6:06 p.m.
    ...biological systems. Size is also a factor; the pol lll promoter and terminator need be encoded only once, and...containing the RNA pol III promoter, gRNA and pol III terminator. These relatively large cassettes (considering...gRNAs that can be packaged together in a single vector. In addition, the pol III promoter is relatively...deletions of other regions, such as noncoding RNAs or regulatory elements, to examine their function. Bacteria...

  2. Click Biology: How to Click and Play to Explore Biology

    Type
    Blog Post
    Published
    Oct. 21, 2025, 1:15 p.m.
    ... post was written by Professor Mark Howarth, investigator at the University of Cambridge. His research...Many of these tools can be found in Addgene's repository (Table 1). Figure 2: Adding function to...the Brainbow system, adds Tetracycline (Tet) transactivator to increase fluorescent protein expression ...can also assemble vaccines, target gene therapy vectors, or lock together smart biomaterials. There are...to particular compartments, or fused to other effectors from nanobodies to fluorescent proteins. So, I...

  3. All in a Twist: dsRNA

    Type
    Blog Post
    Published
    Feb. 2, 2023, 2:15 p.m.
    ... some exist as dsRNA. Still others use dsDNA to store their genetic information. All of these viruses ...sword – balancing antiviral benefits with the inflammatory response associated with the introduction of...of duplexed RNA Mammalian cells have multiple receptors in place to sense dsRNA and respond accordingly...threshold and thus do not typically activate an inflammatory response. However, if dsRNA sensors are mutated...its way outside of the mitochondria, the same inflammatory response can activate. These types of cellular...

  4. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    Published
    June 16, 2022, 1:15 p.m.
    ...tips can help:  If there’s a teacher, mentor, or other educator working with your audience, ask! They ...refers to the work put into creating a permanent store of knowledge (a schema).”  In scicomm, I like to.... It’s a lot easier to read a formulaic fiction story that doesn’t require any detailed retention of information...knowledge and expertise your audience has is a major factor in how quickly they can understand and process ...

  5. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    Published
    April 14, 2022, 1:15 p.m.
    ...credit to. Thankfully, as Addgene's resident blog editor, I could take the needed steps to continue where...alternative reagents have reduced immunogenicity. Effector functions are eliminated, as is antibody recognition...constant region. Nonspecific binding, caused cell receptors for the constant region, is also reduced.  Disadvantages...antibody constant region. They are generated using combinatorial libraries, and high specificity has been reported...allowing for them to be used as intracellular inhibitors.  Aptamers are not proteins at all. These high-affinity...

  6. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    Published
    Oct. 29, 2019, 2:52 p.m.
    ...reporting and genetically encoded fluorescent voltage indicators (GEVI) are ideal tools to track bioelectric states...channelrhodopsin used as a sensitive fluorescent voltage indicator with near-complete desensitization in continuous... artificial intelligence," says Michael Levin, Director of the Allen Discovery Center. The future of this...Use of genetically encoded, light-gated ion translocators to control tumorigenesis." Oncotarget 7.15 (...(2018): 134-144. PubMed PMID: 29684787. Oviedo, Néstor J., et al. "Long-range neural and gap junction ...

  7. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    Published
    Feb. 28, 2017, 3:30 p.m.
    ...expressed in a single vector, pCRISPRainbow-DONOR1, with dCas9 supplied by a separate vector. Figure ...the 4D nucleome. Visualizing specific loci has historically been difficult, as techniques like fluorescent...technique was developed in the lab of Addgene depositor Stanley Qi and used in Chen et al. to track telomere...given chromosome simultaneously, they could also monitor the spatial relationship of the two genes over ...

  8. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type
    Blog Post
    Published
    Jan. 11, 2017, 3:30 p.m.
    ...contributed by guest blogger, Gary McDowell, executive director of Future of Research. On December 1st 2016, many...of Information Act (FOIA) requests for their postdoctoral salary information as of December 1st. Salary...are an important step forward in helping the postdoctoral population continue to pursue academic work,...of the scientific enterprise by reducing the postdoctoral population which keeps growing despite a lack...McDowell! Gary S. McDowell is currently the Executive Director of Future of Research. He is interested in advocating...

  9. Plasmids 101: Modular Cloning

    Type
    Blog Post
    Published
    March 12, 2024, 1:15 p.m.
    ...with a terminator. A simple TU could be comprised of just three parts: promoter, gene, and terminator.   ...As a plasmid repository, we are no strangers to cloning. If you’re reading this, then chances are you...this category also includes promoters, UTRs, terminators, etc. Once selected, individual units can be ...UTRs, genes, tags, etc.) into MoClo-compatible vectors. Level 1: Assembly of multiple Level 0 parts into...

  10. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ... plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles.  Here's ...developed an engineered adeno-associated virus (AAV) vector named AAV.CAP-Mac for non-invasive systemic gene...N.C., Goeden, N. et al. Adeno-associated viral vectors for functional intravenous gene transfer throughout...region VIII of AAV9-X1.1 to that of AAV1. This viral vector has brain endothelial cell-specific tropism, with... by pre-existing antibodies against AAV9-based vectors (and vice versa), enabling repeat dosing. In a ...

  11. Educational Planarian Tools for Teaching Developmental Biology Techniques

    Type
    Blog Post
    Published
    May 24, 2018, 1:20 p.m.
    ... all have a PR-T4 vector backbone, which is a modified version of L440. This vector is versatile in that...of interest into the empty PR-T4P vector backbone. The empty vector PLL4, which is similar to PR-T4P, ...experiments in the beginning. In most classrooms, the laboratory experience is focused on classical embryology...

  12. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    Published
    Nov. 5, 2024, 8:32 p.m.
    ...2002). Insect cells as a factory to produce adeno-associated virus type 2 vectors. Human Gene Therapy, 13...mammalian cells, they do not require serum or an incubator to grow. The virus Insect cell systems are used...references More resources on the Addgene blog Viral Vectors 101: Producing Your rAAV AAV Titers: Where Do They...platform to produce recombinant proteins and viral vectors. Frontiers in Bioengineering and Biotechnology,...

  13. PhD Applications After COVID

    Type
    Blog Post
    Published
    Jan. 5, 2023, 2:15 p.m.
    ...  Is this department/laboratory collaborative with other departments/laboratories? How long is the average...almost as daunting as the program itself. Picking a mentor comes with many challenges, and the flood of interview...more complex. “How do I reach out to potential mentors?” “Where do I start with picking universities?”...by meeting with professors - a/k/a Principle Investigators (PIs) - whose labs does research that aligns...

  14. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

    Type
    Blog Post
    Published
    June 22, 2017, 2:30 p.m.
    ...microscopy, by cloning your protein of interest into a vector encoding a fluorescent tag. Once expressed as a...golgi and then transported in vesicles via the secretory pathway to their subcellular or extracellular ...bacterial components. For example, the Rainbow Vectors from the Mariette Barbier Lab can be used to fluorescently...Edition. 3.  CureFFI.org. Cell Biology 04: The Secretory Pathway. 4. Dunn, Kenneth W., Malgorzata M. Kamocka...Barbier, Mariette, and F. Heath Damron. "Rainbow Vectors for Broad-Range Bacterial Fluorescence Labeling...

  15. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type
    Blog Post
    Published
    June 12, 2014, 3:30 p.m.
    ...is Head of Synthetic Biology at the Sainsbury Laboratory, where she often feels more like an engineer ...the toolkit. We have included a wider range of terminators than are commonly used as we have tested them...system. The paper also gives quantitative data for regulatory elements, which has not been available for plants...easy to express genes in, which makes it a good laboratory workhorse. Tips & tricks Addgene: Do you have...start. They might want to use our promoters and terminators, but then they aren’t sure how to convert their...

  16. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ...unevenly, introducing distortions in your final images. It is possible to keep distortions to a minimum with...done about that.  Second, expansion introduces distortions. The goal in expansion is for your sample to ...polymerizes, and the level of digestion. All of these factors in turn depend upon the specific gel recipe used... L (2022) Super-resolution microscopy: a brief history and new avenues. Philosophical Transactions Royal...

  17. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    Published
    Dec. 7, 2020, 2:53 p.m.
    ...2020). In the two-plasmid system, an effector plasmid (pEffector) expresses all seven required genes and...CRISPR-Cas systems, in contrast to single-protein effectors employed by Type II systems (such as CRISPR-Cas9...plasmid (pDonor) encodes the mini-transposon. pEffector and pDonor can also be combined in single-plasmid...technologies. Message or follow him on twitter @Vopinator.     References: Halpin-Healy TS, Klompe SE, Sternberg...

  18. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    Published
    July 30, 2020, 1:15 p.m.
    ...introduce any one of these topics without some editorializing, and he wisely makes no such attempt. He is...discusses have been making the rounds in journal editorials, science social media, and conference workshops...biology also falls within this section. The GFP story has its own Franklin in Doug Prasher, who originally...describes the detection of gravitational waves--a story I was much less familiar with as a biologist--as...12 years, I’m very familiar with the role our repository has played in these fields, and seeing names ...

  19. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type
    Blog Post
    Published
    Dec. 3, 2019, 11:38 a.m.
    ... in Parkinson’s disease. Knowing the primary investigator where the plasmid was created can narrow your...but I can’t browse through 300+ plasmids. Under ‘Vector Types’ I selected ‘Lentiviral’ which eliminates... coveted Blue Flame is more than an award for depositors and inspiration for our updated mascot, it’s ...model system for your research (e.g. Mammalian) Vector Type: What type of experiment are you planning?...Identify a specific marker protein Use: Similar to “Vector Type” you can filter by experimental purpose (e.g...

  20. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    Published
    Oct. 4, 2018, 12:44 p.m.
    ...CRISPR/Cas9 Synergistic Activation Mediator) uses an improved activator design that consists of inactive... domains that recruit additional transcription factors. The Root and Doench labs utilized tracrRNAs with...stem loops to recruit additional transcription factors to the targeted region (Sanson et al., 2018) . ...tracrRNA was combined with the sgRNA expression vector allowing the screen to be conducted with only two...
Showing: 501 - 520 of 867 results