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We narrowed to 696 results for: Tec;

Showing: 501 - 520 of 696 results

  1. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    Published
    Nov. 15, 2017, 3 p.m.
    ...microscopy of a viral vector solution. Since this technique is intensive and requires an electron microscope...with titrating by qPCR are addressed with ddPCR technology (Hindson et al., 2011, Hindson et al., 2013),...

  2. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    Published
    March 12, 2019, 1:37 p.m.
    ...terms of the question, the methods, the tools or techniques that can help resolve the question, the anticipated... execute the proposal and the tools/resources/technology exist to solve the problem. What will you have...

  3. Lab to Office Culture Shock

    Type
    Blog Post
    Published
    Feb. 5, 2019, 1:04 p.m.
    ...transferable skills. Transferable skills are the non-technical skills you pick up wherever you work, and you’... communicating. After years of focusing on my technical skills with cloning and lentivirus production,...

  4. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    Published
    Oct. 13, 2022, 1:45 p.m.
    ... by directly fusing the cargo to dCas9.  This technique has been exploited to localize factors such as...editors, transposases and prime editors. Nat. Biotechnol. 2020 387 38, 824–844 (2020). Moreno-Mateos, ...

  5. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ...Illumina MiSeq Next Generation Sequencing (NGS) technology. As our repository continues to expand, we realized... programs to a uniform language.  The modern architecture of Nextflow included built-in support for containers...

  6. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    Published
    Oct. 25, 2016, 2:30 p.m.
    ...addition to certain staff scientists and those in technical roles. Therefore, institutions are responsible... law. However postdocs, staff scientists and technicians in primarily bench-science positions are not....

  7. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ... a pCAG-EGXXFP plasmid using standard cloning techniques (The XX indicates where your target is inserted...resulting in the expression of EGFP. Using this technique, you can assess several sgRNA’s at the same time...

  8. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    Published
    Oct. 27, 2015, 3 p.m.
    ...dark pool that is mislocalized to the ER can be detected with immunofluorescence. This population of dark...Sean, and H. R. Pelham. "Use of peptide tagging to detect proteins expressed from cloned genes: deletion ...

  9. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    Published
    May 13, 2025, 1:15 p.m.
    ...commonly employed methods for titering IDLVs include detecting vector particle components or performing quantitative...CRISPR-Cas nucleases in human cells. Nature Biotechnology, 31(9), 822–826. https://doi.org/10.1038/nbt...

  12. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    Published
    June 23, 2016, 2:30 p.m.
    ...#68295 SupD plasmid – Addgene # 68307 For further technical reference and protocols for use of this system...PubMed PMID: 24136966. 8. Tomida, J., et al. "Detection of phosphorylation on large proteins by Western...

  13. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    Published
    Sept. 1, 2015, 2:30 p.m.
    ...research team. For example, if you’re going to need a tech because you’ll be doing mouse experiments, start... current administrative services, managers, and techs’ financial books.  To find personnel salary ranges...

  14. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    Published
    Oct. 2, 2018, 12:56 p.m.
    ...administrative responsibilities? Is the work (topic and/or technical aspects) applicable to industry if this path interests...Questions to ask: Will someone(s) train you technically? Will the PI/supervisor look at your data and...

  15. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    Published
    Oct. 9, 2018, 12:21 p.m.
    ...questions concern great scientific problems, but the technology may not exist to answer the question or it might...teaching experience and learn about modern pedagogy techniques, especially active learning. Serve as a daily...

  16. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ...California, Santa Cruz. Emerging CRISPR/Cas9 editing technologies have transformed the palette of experiments ...countless other systems, whereas import of CRISPR technology into C. elegans has been a transformative innovation...

  17. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    Published
    Nov. 2, 2021, 1:15 p.m.
    ...truncated tyrosine hydroxylase (TH) promoter for catecholamine neurons or glial fibrillary acidic protein (...Considerations For Precise Neuronal Targeting Using AAV Technologies Resources on Addgene.org Find ready to use...

  18. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    Published
    July 6, 2021, 1:28 p.m.
    ... them a great tool for mutagenesis screens that detect loss-of-function or gain-of-function mutations....genome. Then, the transposon insertion sites are detected with next generation sequencing, and analyzed ...

  19. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    Published
    March 14, 2017, 2:30 p.m.
    ...trial phase. In all of the above therapeutics, techniques like genome engineering have played a huge role...cells from using this receptor to evade immune detection. We've only just touched the surface of immunotherapies...
Showing: 501 - 520 of 696 results