We narrowed to 711 results for: ang

...AAV). This issue was recently circumvented by the Yang Lab with the development of a Suntag-p65-HSF1 (SPH...as well as individual neuronal populations.  The Yang Lab’s AAV plasmids used to express Cre under neuron-specific...

...mitotic cells as well as necrotic or dead cells (Jiang and White-Cooper, 2003). Why call a gene required... 139.7 (1997): 1805-1819. PubMed PMID: 9412474. Jiang, Jianqiao, and Helen White-Cooper. "Transcriptional...

... of replication (p15A) worked best under a wide range of inducer concentrations (0.1-1% L-arabinose) without...confluent well of HEK cells in a 6-well dish and change the media every day for three days. GVs should ...

...and blue triangles. 2) An antibody immobilized on a bead binds specifically to the blue triangle protein...

... been converted to an alanine (D10A or H840A), changing its double-strand cut to a single-stranded nick...for your target gene.  Validated gRNAs are a great bang for your buck – they have been used successfully...

...addition, it can be advantageous to speak multiple languages - that makes communication with customers from...requirements (and maybe also the proposed salary range) should help. In smaller companies, technical support...

...digital PCR (ddPCR) titration protocol (modified from Wang et al., 2018). Briefly, 293T cells are transduced...Article 2. https://doi.org/10.1089/hgtb.2013.131 Wang, Y., Bergelson, S., & Feschenko, M. (2018). Determination...

...watched each month, in 39 countries, and in over 54 languages. While most scientific videos were for entertainment...researchers for whom English is a second or third language (making publishing their research all the more...

...supporting datasets must be logically annotated and arranged, as in an actual publication and not require interaction...take interest in how it is released, we will be in danger of having to deliver on well-meaning but impractical...

... Choosing the promoter Mature siRNAs typically range from 19–22 nucleotides and are 100% complementary... Taxman, D. J., Moore, C. B., Guthrie, E. H., & Huang, M. T. (2010). Short hairpin RNA (SHRNA): Design...

...for researchers looking to generate long-lasting changes in cells. However, for some applications, integration...construct has the D64V mutation, a single amino acid change (from aspartic acid to valine at position 64) which...

...the nuances of the research in as technical of language as possible. Seriously. My suggested litmus test...knowledge that you have given everyone a simple plain language map of where you are going and what you expect...

...lamps are the best for working in this spectral range. You can also check that no filters and/or cold ...tell us about cellular events via  fluorescence changes occurring due to protein synthesis, degradation...

...techs’ financial books.  To find personnel salary ranges, go to online job information sites (like, indeed.com...focused on research relevant objectives and their tangible resources. For example, if a dean/chair asks “...

... a wide variety of genomic edits — single-base changes, small deletions, and large insertions up to 12... B., Tran, T., … Dimidschstein, J., Fishell, G., Wang, Y. (2024). An enhancer-AAV toolbox to target and...

...palette for display (like green-magenta-blue or orange-blue rather than red-green). Even better, show ..., Ferling, I., Gazda, M. A., Hoeppner, L. H., Ilangovan, V., Lo, H., Olson, M., Mohamed, S. Y., Sarabipour...

...s chromatin structure undergoes significant rearrangement (such as when a stem cell initiates differentiation...researchers to avoid making unnecessary base pair changes as would be the case with ssODNs. One minor drawback...

...McClinntock who first discovered transposons due to changes in coloration of corn kernels. Aside from corn,... et al., 2021). Target site preference and host range are important to consider when selecting a transposon...

...Make one yourself. Instead of thinking “What’s my angle on attacking this problem?”, try thinking “Who can...can I assemble to attack this problem from every angle?” Your science will be better for it. Additional...

...retroviral vectors because they target a broader range of cells and their integration into the genome is...you put in your culture. As viral production can change from one batch to another. For experimental consistency...