...bacteria, so it can be transformed as-is into cells. The cells then get to work fixing up the “damage” and...nicked plasmid together long enough to get it into cells and let them do the repair work. The key is T4 DNA...

...they either trace downstream from the soma (neuron cell body) toward the axon (anterograde tracer) or upstream...particles require receptor-mediated entry into the cell. This means that not all neurons and pathways will...chemogenetic vector directly into the region where the cell bodies of the projection neurons reside.   Impact...

...quantitatively evaluating antibody staining, or counting cells are now routine, open-source software projects that...portable device for studying plankton using flow cells, has become an established alternative to high-priced... and blueprints has the potential not only to accelerate scientific progress, but also to enable science...

...20, 2021. By Will Arnold and Shreya Vedantam Accelerating research by improving the availability of materials...plasmids to help academic and industrial labs accelerate research and to help find a faster solution to...modification may not be easily expressed in non-mammalian cells. Yeast expression plasmids The yeast expression ...

...electrocompetent cells in which the phage recombination machinery is induced using L-arabinose. These cells can be...Enzymes Required No No Yes Yes Lab Made Competent Cells Required Yes No No No Comparison of REPLACR-mutagenesis...

...from peer-reviewed publishing in a journal.  Accelerating Scientific Communication Preprints have been...Fig. 1: Stand aside, CERN - it's a new age of accelerating science! Photo courtesy of under a CC license...Lacy was also employed by the American Society for Cell Biology in a position working with preprints in ...

...Professional Career Coaching can be an excellent tactic for scientists making a career shift or who wish...very popular speaker and workshop leader. Lauren Celano of Propel Careers is not a professional coach but...Consulting is part of that group and she is especially excellent in coaching for communication skills. Industry...

...encode proteins. They play important roles in many cellular and regulatory processes, but much of their functionality...screening for functional long noncoding RNAs in human cells by Cas9 targeting of splice sites. Nat Biotechnol...non-coding RNAs with the CRISPR/Cas9 system in human cell lines. Nucleic Acids Research. 2014;43(3):e17. doi...

...Drosophilia Plasmid pRDB_051 (for stable fly cell lines; attL and attR sequences for genome integration...Drosophilia Plasmid pRDB_052 (for stable fly cell lines; Hygromycin resistance gene; for genome integration...rapid assessment of emerging CRISPR technologies. Cell Genomics, 4(3), 100519. https://doi.org/10.1016/...

...currently doing this using two approaches: single-cell RNA-sequencing to identify neuronal class and deep...WDDD aims to acquire quantitative data on all the cell divisions of the C. elegans embryo when all essential...Wang et al., 2018).” With the split system, only cells that have both halves of cGAL can drive expression...

...origin of cellular life. His lab has long hypothesized that life started with RNA and a simple cell membrane... circular RNA they have identified resulted in a cell migration defect. They have created a circRNA website...

...overexpress YFG in a Drosophila cell line. Knock downs in Drosophila cell lines are extremely effective....person. Animals are diploid except for their germ cells (sets of genomic material are contributed by mother...

...identifying new genes required for stem and progenitor cell maintenance in planarians in Developmental Biology... his teaching style, with the former offering excellent training in improvisation and the latter making...junction complex is required for stem and progenitor cell maintenance in planarians. Developmental Biology...

...triple colocalization is possible even when staining cells with three IgG1 antibodies. Antibodies were individually...workflow yields similar localization patterns as cells stained for one target (compare nanobodies staining...–rabbit IgG secondary nanobodies. The Journal of Cell Biology. PubMed PMID: 29263082 Find plasmids from...

... smaller cargos, and the ability to easily cure cells of the plasmid after integration. Figure ...into the mini-transposon Transform plasmid(s) into cells and plate overnight Screen colonies phenotypically...distinct biological elements, CRISPR-transposons excel at programmable insertions of even very large genetic...

...useful addition to the toolkit for visualizing cellular processes in C. elegans: mScarlet3 for applications...biologically relevant forces at focal adhesions in living cells in several different contexts.   Figure ...substrate furimazine. Right: Demonstration of PILATeS. Cell adhesion to a PILATeS-functionalized coverslip creates...

...As part of Addgene’s 20th anniversary celebration, we’ve been finding and sharing stories unique to our... be safely used to deliver a genetic payload to cells, and the complexity of effectively deploying them... can be to use. AAVs, which can target different cell types depending on their serotype, are particularly...

...of experience in [field of research], and have excellent [list the most important skills for the position...your specific skills and experience will help you excel in the role. It can also convey your excitement ... specific examples. Instead of saying “I have excellent communication skills” try giving an example, like...

...score (KO-score) which represents the proportion of cells that have either a frameshift or 21+ bp indel. This...Following delivery of CRISPR components into target cells, genomic DNA from both edited and unedited (control... score. KO Score - Represents the proportion of cells that have either a frameshift or 21+ bp indel. This...

...example, "while it is possible to grow liver stem cells and liver-like mini-organs or organoids, neither...combine bioengineered scaffolds with patient stem cells to generate compatible functional livers.” Why are... optimization of fluorescent proteins,  and live cell imaging approaches. Erik is also an avid long distance...