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We narrowed to 793 results for: lat

Showing: 501 - 520 of 793 results

  1. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    Published
    Jan. 25, 2024, 2:15 p.m.
    ...sequence (NGG, etc.) as well as their orientation relative to the gRNA (5’ or 3’). In some cases, there is... used by Jinek et al. 2012, or browsing for the latest AsCas12a plasmids, Addgene and CasPEDIA have got...to browse entries by the current phylogenetic nomenclature and discover orthologs with similar properties...

  2. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    Published
    March 21, 2023, 1:15 p.m.
    ...scientific and medical tools. As scientists, we can manipulate what this cargo is and where it goes, turning...mutagenesis in the host. Adenoviruses can deliver relatively large cargo inserts (>8 kb). Adeno-associated...their low immunogenicity, which means they do not stimulate a strong immune response when introduced into ...

  3. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    Published
    April 5, 2022, 1:20 p.m.
    ...plasmids for expression of mAbs, the Trimmer lab isolated RNA from cryopreserved hybridomas, immortalized...then used to generate cDNA, which  was used as a template for PCR amplification with a degenerate primer...grown in tissue culture, they were also able to isolate RNA from non-recoverable hybridoma stocks, without...

  4. Hot Plasmids - February 2022

    Type
    Blog Post
    Published
    Feb. 22, 2022, 2:15 p.m.
    ...miniature CRISPR-Cas system for mammalian genome regulation and editing Anti-FLAG Frankenbodies for modular... genes, researchers at the Genetic Pertubation Platform at the Broad Institute have expanded the genetic...miniature CRISPR-Cas system for mammalian genome regulation and editing by: Rachel Leeson The large size ...

  5. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    Published
    Feb. 2, 2021, 2:15 p.m.
    ...certain to choose your recommenders based on your relationships to the person as well as their current sphere...just as you would for an in-person interview. Formulate and practice a set of common questions with a ...academic resources among economically disadvantaged populations. Roo is the co-founder of STEAMid.       Rose...

  6. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    Published
    May 26, 2020, 1:15 p.m.
    ..., integrated reporter for assaying CRISPR/Cas-stimulated HDR activity) The Madison lab created SRIRACCHA...Joung JK (2014) CRISPR-Cas systems for editing, regulating and targeting genomes. Nature Biotechnology 32...integrated surrogate reporter for assaying CRISPR/Cas9-stimulated homology-directed repair. Journal of Biological...

  7. Developing a cold tolerant E. coli using specialized chaperones

    Type
    Blog Post
    Published
    Jan. 28, 2020, 2:35 p.m.
    ...contained cold tolerant chaperones (the Cpn 10/60 isolated from Oleispira antarctica, and the P. haloplanktis...psychrophilic systems as well as engineering and regulating psychrophilic recombinant proteins.  To infinity...2019 All-India iGEM Meet-up, which served as a platform for all the Indian iGEM teams to interact with...

  8. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

    Type
    Blog Post
    Published
    July 28, 2015, 2:30 p.m.
    ...effort to track down every last one of those gene regulatory proteins and the places in the genome where they...closely together on several projects and this is one related to gene expression. One goal of the ENCODE project...resistance to neomycin so that you can select the population of cells with the tagged transcription factor...

  9. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

    Type
    Blog Post
    Published
    July 10, 2014, 7:17 p.m.
    ...community. As a result, I have occasionally pondered translating my research into a startup company. However, ...effort in preparation and practice is directly related to success presenting." A journey from graduate...LabSpaces Blog Find a Buisness Plan Competition Good Relations - PR for the Biotech Business - Posted on Nature.com...

  10. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    Published
    Dec. 10, 2013, 3:13 p.m.
    ... the cell with a mutant template and fooling the cell into using that template for repair. By using these...the two ends back together or using a homologous template provided by the sister chromosome. As cells take...

  11. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

    Type
    Blog Post
    Published
    March 19, 2019, 1:31 p.m.
    ...expression cassettes on a single plasmid (Grefen and Blatt, 2012). Similar to traditional Gateway™ cloning,...Fluorescence Complementation (rBiFC, Grefen and Blatt, 2012). The 2in1 Plasmid Toolkit consists of 4 plasmid...Biol, 2018. PubMed PMID: 29043675.Grefen C and MR Blatt. Biotechniques 2012. PubMed PMID: 23066669.Hecker...

  12. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    Published
    Feb. 28, 2017, 3:30 p.m.
    ...distances of 2 and 75 Mbp, with the calculated fluorescent distances correlating with the previously established...simultaneously, they could also monitor the spatial relationship of the two genes over time, at a range of distances...

  13. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type
    Blog Post
    Published
    Nov. 3, 2020, 2:15 p.m.
    ...amplitude, wavelength sensitivity, and off-kinetics correlated with the model’s predictions.  Three top performers...reach the VTA. Mice were then allowed to “self-stimulate” their VTA dopaminergic neurons by triggering ...H134R) expressing mice did not have this self-stimulation response. This result showed that ChRger2 stronger...

  14. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    Published
    Dec. 8, 2022, 2:15 p.m.
    ...’t just for DNA anymore! Cas enzymes have been isolated which target and cleave RNA through the same mechanism...and 2). cell toxicity. Cas7-11 does not display collateral RNA cleavage and is significantly less toxic ...mammalian cells than Cas13 (likely due to the lack of collateral cleavage). You can read about Cas7-11 in our ...

  15. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    Published
    April 20, 2023, 4 p.m.
    ...string together all the words that I could remember related to this paper and/or biology in general until I... turn your slide deck into a chalk talk. Your translator doesn’t know the term “diving board” so you end...or talk extremely quickly. You realize, much too late, that you labeled your gene of interest incorrectly...

  16. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    Published
    March 15, 2022, 1:15 p.m.
    ...pathway is regulated. You’ve read all the background papers you could get your hands on, formulated a hypothesis...immunoprecipitation-mass spectrometry strategies that isolate an antibody’s immune complexes and confirm interacting...

  17. What's New in CRISPR - November 2021

    Type
    Blog Post
    Published
    Nov. 18, 2021, 2:15 p.m.
    ...available at Addgene. If you want to read more content related to CRISPR technology, subscribe to our CRISPR blog... plasmids Enhanced prime editing systems by manipulating MMR machinery In their Cell paper, the Liu Lab...other prime editing tools, the desired edits are templated by the 3′ extension of the pegRNAs. PEDAR is capable...

  18. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    Published
    July 21, 2016, 2:30 p.m.
    ...“Fix the Knowledge” or “gendered innovations” stimulates excellence in science and technology by integrating...interventions you develop represent the whole human population. To learn a little more about where differences...clinical trials. And, as Janine Clayton points out, late-stage clinical studies that draw from a body of ...

  19. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    Published
    Jan. 29, 2015, 1:25 p.m.
    ...scientists are aware of the benefits of coaching relationships and do not know where to look for a coach. Many...thinking about what your goals are for the coaching relationship. Do you want to change jobs? Grow your professional...the coach to define how you will know that the relationship has been successful – what are the desired outcomes...

  20. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type
    Blog Post
    Published
    Oct. 22, 2019, 12:20 p.m.
    ...The UCLA Library offers some Powerpoint based templates with different column numbers for a good starting...starting point. Adobe also offers free poster templates, although most are vertical.  Large central box...is relevant to the work you are presenting. The latter is catchy, but beware. A distracting background...
Showing: 501 - 520 of 793 results