We narrowed to 644 results for: rest

1. Walking beside Academia and giving it a high five: My transition from graduate work to a position at Addgene

   Type

   Blog Post

   Published

   July 2, 2015, 8:37 p.m.

   ...content that scientists will find useful and interesting than that will simply get people ordering plasmids...

2. Management for Scientists: Giving Feedback

   Type

   Blog Post

   Published

   Feb. 6, 2015, 2:46 p.m.

   ...is possible in your eyes so their confidence is restored. A good manager does not hold grudges. Never...

3. The Stingy Scientist: How the Baby Gel Box Was Born

   Type

   Blog Post

   Published

   April 15, 2014, 3:11 p.m.

   ... reagents and money.  This was the start of my interest in “stingy science”.  Practicing thrift and conservation...

4. Plasmids 101: Antibiotic Resistance Genes

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   Blog Post

   Published

   Jan. 30, 2014, 3:29 p.m.

   ...separate resistance cassettes and your gene of interest is cloned into/inactivates or completely removes...

5. Choosing a Good Mentor for Scientists

   Type

   Blog Post

   Published

   Jan. 16, 2014, 2:56 p.m.

   ...about workplace bullying featured on the always interesting Tenure, She Wrote blog. Unfortunately, academic...

6. History of CRISPR Cas - A tale of survival and evolution

   Type

   Blog Post

   Published

   Nov. 25, 2013, 10:12 p.m.

   ...viral anti-CRISPR genes. These data highlight an interesting "arms race" between phages and prokaryotes; the...

7. CRISPR Challenges: Standardization and Homology Directed Repair

   Type

   Blog Post

   Published

   Feb. 14, 2018, 2:36 p.m.

   ...directed repair (HDR) are echoed in the amount of interest in base editors, which alter genomic DNA without...

8. Hot Plasmids and Viral Preps - September 2021

   Type

   Blog Post

   Published

   Sept. 21, 2021, 1:15 p.m.

   ...required three components: a master mix, the DNA of interest, and cell extract.    Graphical Abstract...

9. Fluorescent Biosensors for Measuring Autophagic Flux

   Type

   Blog Post

   Published

   Jan. 22, 2019, 2:41 p.m.

   ...mitophagy. Rosella can also be fused to a protein of interest to track the autophagy of that protein. Find ...

10. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type

    Blog Post

    Published

    Sept. 21, 2023, 1:15 p.m.

    ...use two antibodies - one against your target of interest and a second against the first antibody. When ...

11. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type

    Blog Post

    Published

    Aug. 31, 2023, 1 p.m.

    ...would be wireless, able to monitor outcomes of interest, and stimulate or manipulate responses through...

12. Antibodies 101: Epitope Tags

    Type

    Blog Post

    Published

    May 11, 2023, 1:15 p.m.

    ...the lookout for aggregation of your protein of interest!   Ready to start epitope tagging?   References...

13. The Strength of Story Telling

    Type

    Blog Post

    Published

    Dec. 19, 2017, 2 p.m.

    ... my job I most enjoy is building and telling interesting stories — whether I’m working with a talented...

14. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type

    Blog Post

    Published

    June 9, 2016, 2:30 p.m.

    ... also need to be transfected into the cells of interest. In contrast, RNA guides must be expressed from...

15. Addgene's Top 10 Blog Posts of 2015

    Type

    Blog Post

    Published

    Dec. 7, 2015, 6 p.m.

    ...strains you can use to express your protein of interest. 4. Plasmids 101: Blue-White Screening Getting...

16. Learning Technical Skills at a Lab Central Workshop

    Type

    Blog Post

    Published

    Feb. 20, 2018, 2:30 p.m.

    ...Komuhendo is a Lab Technician at Addgene who is interested in medicine and music.       Additional Resources...

17. DAP arrays for multiplex gene editing

    Type

    Blog Post

    Published

    July 7, 2022, 1:15 p.m.

    ...editing, in virtually any cell type or organisms of interest with only minimal genetic payloads. Different ...

18. Improving Transduction Efficiency with Exosome AAVs

    Type

    Blog Post

    Published

    March 14, 2019, 1:54 p.m.

    ...vehicle for drug delivery that has recently gained interest special contexts, such as the delivery of therapeutic...

19. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type

    Blog Post

    Published

    Oct. 26, 2017, 1:59 p.m.

    ...and if you have any tips, input, or stories of interest about library use, let us know in the comments...

20. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type

    Blog Post

    Published

    June 20, 2017, 2:30 p.m.

    ... is opened and equilibrium with ambient air is restored. We have no evidence to suggest that the transient...