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Showing: 501 - 520 of 687 results

  1. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ... a pCAG-EGXXFP plasmid using standard cloning techniques (The XX indicates where your target is inserted...resulting in the expression of EGFP. Using this technique, you can assess several sgRNA’s at the same time...

  2. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    Published
    Oct. 27, 2015, 3 p.m.
    ...dark pool that is mislocalized to the ER can be detected with immunofluorescence. This population of dark...Sean, and H. R. Pelham. "Use of peptide tagging to detect proteins expressed from cloned genes: deletion ...

  5. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    Published
    May 13, 2025, 1:15 p.m.
    ...commonly employed methods for titering IDLVs include detecting vector particle components or performing quantitative...CRISPR-Cas nucleases in human cells. Nature Biotechnology, 31(9), 822–826. https://doi.org/10.1038/nbt...

  6. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    Published
    June 23, 2016, 2:30 p.m.
    ...#68295 SupD plasmid – Addgene # 68307 For further technical reference and protocols for use of this system...PubMed PMID: 24136966. 8. Tomida, J., et al. "Detection of phosphorylation on large proteins by Western...

  7. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    Published
    Sept. 1, 2015, 2:30 p.m.
    ...research team. For example, if you’re going to need a tech because you’ll be doing mouse experiments, start... current administrative services, managers, and techs’ financial books.  To find personnel salary ranges...

  8. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    Published
    Oct. 2, 2018, 12:56 p.m.
    ...administrative responsibilities? Is the work (topic and/or technical aspects) applicable to industry if this path interests...Questions to ask: Will someone(s) train you technically? Will the PI/supervisor look at your data and...

  9. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    Published
    July 6, 2021, 1:28 p.m.
    ... them a great tool for mutagenesis screens that detect loss-of-function or gain-of-function mutations....genome. Then, the transposon insertion sites are detected with next generation sequencing, and analyzed ...

  10. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ...California, Santa Cruz. Emerging CRISPR/Cas9 editing technologies have transformed the palette of experiments ...countless other systems, whereas import of CRISPR technology into C. elegans has been a transformative innovation...

  11. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    Published
    Nov. 2, 2021, 1:15 p.m.
    ...truncated tyrosine hydroxylase (TH) promoter for catecholamine neurons or glial fibrillary acidic protein (...Considerations For Precise Neuronal Targeting Using AAV Technologies Resources on Addgene.org Find ready to use...

  12. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    Published
    Oct. 9, 2018, 12:21 p.m.
    ...questions concern great scientific problems, but the technology may not exist to answer the question or it might...teaching experience and learn about modern pedagogy techniques, especially active learning. Serve as a daily...

  13. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    Published
    March 14, 2017, 2:30 p.m.
    ...trial phase. In all of the above therapeutics, techniques like genome engineering have played a huge role...cells from using this receptor to evade immune detection. We've only just touched the surface of immunotherapies...

  14. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ...MAGE (Multiplex Automated Genome Engineering) technology. pMA7CR_2.0 expresses lambda Red and Cas9, which...for highly specific genome editing.”  Nature Biotechnology 32(6) (2014): 569-576. PubMed PMID: 24770325...

  16. Plasmids 101 eBook 4th Edition

    Type
    Blog Post
    Published
    Sept. 5, 2023, 1:15 p.m.
    ...plasmids parts and nomenclature to popular cloning techniques and troubleshooting tips. The fourth edition ...

  17. Addgene's 20th Anniversary Party

    Type
    Blog Post
    Published
    June 11, 2024, 1:15 p.m.
    ...from facilities management to website development, tech transfer, lab work, and more. It's this diverse ...

  18. New FLIS Plasmid (April Fools 2022)

    Type
    Blog Post
    Published
    April 1, 2022, 1:15 p.m.
    ...working in fly research, I hope you find this new technology as helpful as we have!  References and Resources...

  20. New Videos: Addgene Lab Tips

    Type
    Blog Post
    Published
    Feb. 15, 2018, 2:41 p.m.
    ...your life a little easier. These can range from technical tips as in our episode on Barcoding, to basic ...
Showing: 501 - 520 of 687 results