We narrowed to 754 results for: tro

...Each plasmid page now has a helpful graphic to introduce you to the plasmid class covered on that page....Using CRISPR/Cas9 and Plasmids 101: NGS Quality Control for Pooled Libraries Day 12: New website resources...

...researchers in developing countries. We are proud to introduce the 5 scientists who were awarded the Addgene-...technology to engineer plants that are able to destroy DNA viruses, similar to the anti-phage CRISPR effect...

...a reporter signal. Reporter signals may be electrochemical or light-based, with luminescent and fluorescent... being especially popular. We’ll give you an introduction to fluorescent biosensors, but keep in mind ...

...You’ve designed the perfect experiment – controls, conditions, and everything in between – now all you... Susanna Stroik...

...   Listen this episode of Hot Plasmids! Light controls the activity of a microtubule-associated protein...studying cellular dynamics. The photoinducible control of protein function is more specific and much faster...

...surprised to see that it would open not with an introduction to CRISPR nucleases, but with a session on DNA...editing rates across multiple systems. Read our introductory blog post, then check out this RNP protocol ...

...endogenous IgGs, you should include no primary antibody controls in your experiments. If you do find high levels...resources on the Addgene blog Antibodies 101: Introduction to Immunofluorescence Antibodies 101: Affinity...

...Culture Adenoviral Vector Production and Troubleshooting Additional resources on Addgene.org AAV ...  Susanna Stroik...

...packed with tech transfer resources (summaries, electronic MTA approval, archive of old MTAs, and more) ... Transfer Information Tech Transfer News: A Reintroduction to the UBMTA ...

...cell types. MEFs were reprogrammed into iEPs by retroviral expression of transcription factors Foxa1 and.... Adding Mettl7a1 to the standard Foxa1-Hnf4α retroviral overexpression cocktail yielded a three-fold ...

... A fluorescent probe that releases an internal control Another autophagy biosensor is the GFP-LC3-RFP-LC3ΔG...it remains in the cytosol acting as an internal control. Autophagic flux is estimated by the GFP/RFP ratio...

...elegans, they inserted the SEC into a synthetic intron between a fluorescent protein (FP) and a 3xFLAG...resulting loxP scar is located in this synthetic intron, and only the fluorescent protein and 3xFLAG-tag...

...build, sales and marketing gives you a great introduction to the financial world. It teaches you about...you can find some great information on ELISA troubleshooting, ELISA kits and cell death assays. You can ...

...build circuits in physics class to learn about electronics or mix chemicals together in chemistry to see... way into high school classrooms, like gel electrophoresis to separate DNA or transforming cells with ...

...YOUR PLASMID has been developed to teach gamma-retrovirus or lentivirus-based ex-vivo gene therapy’ strategies...at FRESCI particularly interested in advanced in vitro models technology. Follow him on twitter @FRE_SCI...

... the lab, so in December 2015 we held our first intro to bacterial transformation course (read our blog...bacterial transformation guru and instructor for our intro to biohacking courses. Our members pursue a diverse...

...necessarily have to be. Regions of homology can be introduced to fragments via PCR using primers that contain...patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc. Many thanks to ...

...Nsp5. Our set also includes a GFP construct as a control. For easier portability, all viral genes are flanked...clone selection. Expression is driven by a fairly strong EF1alpha promoter. We have verified expression ...

... one figure. If you aren’t there yet, write introductions to potential publications or prepare a review...isn’t for a while. Getting started early on introductions and literature reviews could make the months...

...complex genetic diseases, Alzhiemer’s disease (AD).  Troy Rohn and his lab are researching a way to treat ...proteins aid the limitation of these mutations, to control the CRISPR system activity.  Using an AAV vector...