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We narrowed to 772 results for: INA

Showing: 261 - 270 of 772 results

  1. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Sept. 24, 2020, 1:15 p.m.
    ...Originally published May 3, 2017 and last updated Sep 24, 2020 This post was contributed by guest blogger...generally have fewer sequences to choose from). Finally, for HDR, location is much more important because...control, one should revert the edit back to the original in order to prove that the phenotype was really...activation and inhibition by CRISPRa and CRISPRi Finally, for modulating gene expression at the level of...ability to use sequence-based and other features to nominate gRNAs that are likely to be active, not only for...

  2. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    Published
    Nov. 6, 2015, 3:30 p.m.
    ...and should not be confined by birth country or financial means. Instead, talent should be realized through...experience from talented professionals to their original countries to help build, together with the universities...globalized environment re-emerging from international financial struggle, our new generations feel thrown, to ...students who participated in the program in its inaugural year: Joaquin Lilao (Mentee IMP 2014-15, University...interviews, expanded profesional networks, and nominations for government fellowships. The closing and formal...

  3. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    Published
    Oct. 9, 2018, 12:21 p.m.
    ...fellowship grants) A project with sufficient preliminary data to establish your own lab and get an independent...goals. Knowing when to follow a lead and when to terminate a project are fundamental skills that all group...their research. They dread giving a departmental seminar or a joint lab meeting. I've been told that PFs... research fits in with the current literature. Finally, if you give your talk or poster to enough different...hiking, and photography to be very satisfying. Finally, many PFs are in committed relationships and/or...

  4. Plasmid Preps: Different Purity, Different Quantities, Different Uses

    Type
    Blog Post
    Published
    June 16, 2020, 1:25 p.m.
    ... a set of techniques that are used to insert recombinant DNA from prokaryotes or eukaryotes into a synthetic...(genomic DNA and RNA), proteins and chemical contaminants that have been used during the purification ...scientists an indication of purity versus protein contaminants. The 260 and 230 nm ratios should fall ~2 - ... being free of any nucleic acid and chemical contaminants, these DNA samples must also be entirely endotoxin...

  5. Making Career Development an Integral Part of the Science Training System

    Type
    Blog Post
    Published
    Feb. 25, 2021, 2:15 p.m.
    ... science graduate programs are still doing an inadequate job of supporting science trainee transition ...captured in this just released report “Enhancing Dissemination of Evidence-Based Models for STEM PhD Career...to identify the most effective practices and disseminating career training approaches in a way that can...professional development are intended to be widely disseminated by “train the trainer” programs.  Please join...

  6. 6 Tips for Analyzing and Troubleshooting Sanger Sequencing Results

    Type
    Blog Post
    Published
    June 26, 2014, 2:23 p.m.
    ... This blog was originally published on BitesizeBio here. As part of my job ensuring plasmid quality at...sequence Anything less and you might suspect contamination in your sample or consider asking your sequencing...but well worth it. 6. Edit your DNA sequence Finally, when you do see a miscalled peak, don’t be shy... Acknowledgements: Thank you BitesizeBio for originally publishing this and allowing us to share it with...

  7. What's New in CRISPR - Spring 2019

    Type
    Blog Post
    Published
    March 21, 2019, 12:47 p.m.
    ...plasmids  Plant base editing with a Cas9-adenosine deaminase fusion Caixia Gao’s lab has developed a new plant...plant base editor from an evolved tRNA adenosine deaminase that is fused to a nicking Cas9. This base editor...it can also be regulated spatially with Cre-recombination. Read the paper in Frontiers in Molecular ...an alternative to the conventional Cre or Flp recombinases used to excise markers. It has the advantage...

  8. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    Published
    Aug. 28, 2018, 12:04 p.m.
    ...with a confocal microscope, yet I was once again fascinated by the world that reveals itself when looking... both interesting and approachable!   The grand finale of visual impressions was delivered by the ASM ...ASM Agar Art Winners! It is absolutely fascinating to see the living art designed by creative minds with...steady hand. By connecting art and science, a fascinating interface is created that produces loads of inspiration...

  9. Stem Cell Models for Disease & Open Science: Interview with Darrell Kotton

    Type
    Blog Post
    Published
    July 7, 2017, 1:32 p.m.
    ...Darrel does 20% clinical work 80% research and was originally focused on becoming an MD but later got obsessed... therapy, and tissue engineering. How the multidisciplinary nature of the Center for Regenerative Medicine...lungs are difficult, but other tissues like the retina have cell-based therapies underway. Chapter 7: ...Additional Resources from Addgene Learn about Retinal Gene Therapy from Connie Cepko Find Plasmids from...

  10. Quick, Versatile Plant Transgenesis with GreenGate Plasmids

    Type
    Blog Post
    Published
    Jan. 23, 2014, 3:23 p.m.
    ...promoters, N-terminal tags, coding sequences of the gene of interest, C-terminal tags, plant terminators and resistance...percent efficiency, simply stopped working. They finally traced the problem back to the endonuclease. “It...
Showing: 261 - 270 of 772 results